pH-Dependent binding analysis, a new and rapid method for isoelectric point estimation

Analytical Biochemistry

Volumn 147, Issue 1, 1985, Pages 148-155

  Yang, Victor C ^a   Langer, Robert ^a,b  

^a MASSACHUSETTS INSTITUTE OF TECHNOLOGY   (United States)

^b BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

ampholyte; chromatofocusing; isoelectric focusing; isoelectric point; microheterogeneity; pH dependent binding analysis

Indexed keywords

BUFFER; PROTEIN;

ARTICLE; BINDING SITE; ISOELECTRIC POINT; PH; ULTRAVIOLET SPECTROPHOTOMETRY;

BINDING SITES; BUFFERS; HYDROGEN-ION CONCENTRATION; ISOELECTRIC POINT; PROTEINS; SPECTROPHOTOMETRY, ULTRAVIOLET; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0022426042     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/0003-2697(85)90021-1     Document Type: Article

References (30)

1. Isoelectric Fractionation, Analysis, and Characterization of Ampholytes in Natural pH Gradients. I. The Differential Equation of Solute Concentrations at a Steady State and its Solution for Simple Cases.
2. Isoelectric Fractionation, Analysis, and Characterization of Ampholytes in Natural pH Gradients. II. Buffering Capacity and Conductance of Isoionic Ampholytes.
3. Isoelectric points and molecular weights of proteins
4. Heterogeneity of 125I-labeled tetanus toxin in isoelectric focusing on polyacrylamide gel and polyacrylamide gel electrophoresis
5. Isoelectric points of the polysaccharide-degrading enzymes in Flavobacterium heparinum.