A limiting dilution assay for quantifying Leishmania major in tissues of infected mice

Parasite Immunology

Volumn 7, Issue 5, 1985, Pages 545-555

  TITUS, R G ^a   MARCHAND, M ^b   BOON, T ^b   LOUIS, J A ^a  

^a UNIVERSITY OF LAUSANNE   (Switzerland)

^b International Institute of Cellular and Molecular Pathology   (Belgium)

Author keywords

Leishmania major; limiting dilution assay; T cells

Indexed keywords

ANIMAL CELL; ANIMAL EXPERIMENT; DIAGNOSIS; DILUTION; LEISHMANIA MAJOR; METHODOLOGY; MOUSE; NONHUMAN; PARASITE IDENTIFICATION; PRIORITY JOURNAL; PROTOZOON;

ANIMAL; IMMUNIZATION, PASSIVE; LEISHMANIA TROPICA; LEISHMANIASIS; LYMPH NODES; METHODS; MICE; MICE, INBRED BALB C; MICE, INBRED CBA; SUPPORT, NON-U.S. GOV'T; T-LYMPHOCYTES; TIME FACTORS;

EID: 0022234330     PISSN: 01419838     EISSN: 13653024     Source Type: Journal    
DOI: 10.1111/j.1365-3024.1985.tb00098.x     Document Type: Article

References (15)

1. Leishmania tropica: Pathogenicity and in vitro macrophage function in strains of inbred mice
2. An easily prepared defined medium for cultivation of Leishmania donovani promastigotes
3. Thymoma production of T‐cell growth factor (Interleukin 2)
4. The estimation of bacterial densities from dilution series
5. Murine cutaneous leishmaniasis: disease patterns in intact and nude mice of various phenotypes and examination of some differences between normal and infected macrophages
6. Advantages of measuring changes in the number of viable parasites in murine models of experimental cutaneous leishmaniasis
7. Recognition of protozoan parasite antigens by murine T‐lymphocytes. I. Induction of specific T‐lymphocyte dependent proliferative response to Leishmania tropica.
8. Recognition of protozoan parasites by murine T‐lymphocytes. II. Role of the H‐2 gene complex in interactions between antigen presenting macrophages and Leishmania‐immune T‐lymphocytes
9. The in vitro generation and functional analysis of murine T‐cell populations and clones specific for a protozoan parasite, Leishmania tropica.
10. The quantification of viable Leishmania enriettii from infected guinea‐pig tissues
11. Immunology of clinical and experimental leishmaniasis.
12. Limiting dilution dilution assays for the determination of immunocompetent cell frequencies. I. Data analysis
13. Exacerbation of murine cutaneous leishmaniasis by adoptive transfer of parasite‐specific helper T‐cell populations capable of mediating Leishmania major‐specific delayed‐type hypersensitivity
14. The effect of BCG on experimental cutaneous leishmaniasis in mice