Charge transfer between fluorescein and tryptophan as a possible interaction in the binding of fluorescein to anti-fluorescein antibody

Molecular Immunology

Volumn 22, Issue 1, 1985, Pages 45-55

  Templeton, Eva F Gudgin ^a   Ware, William R ^a  

^a UNIVERSITY OF WESTERN ONTARIO   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

FLUORESCEIN; IMMUNOGLOBULIN G ANTIBODY; TRYPTOPHAN;

INTERACTION; METHODOLOGY; NONHUMAN; PRIORITY JOURNAL;

AMINO ACIDS; ANTIGEN-ANTIBODY REACTIONS; FLUORESCEIN; FLUORESCEINS; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN G; SPECTROMETRY, FLUORESCENCE; SUPPORT, NON-U.S. GOV'T; TEMPERATURE; TRYPTOPHAN;

EID: 0021921132     PISSN: 01615890     EISSN: None     Source Type: Journal    
DOI: 10.1016/0161-5890(85)90033-1     Document Type: Article

References (17)

1. Structure of antifluorescein combining sites in rabbit and sheep: induced circular dichroism of bound haptens
2. Direct observation of monophotonic photoionization in tryptophan excited by 300 nm radiation. A laser photolysis study
3. The combining site of the dinitrophenyl-binding immunoglobulin A myeloma protein MOPC 315
4. Relaxation methods
5. Sequential appearance of three different anti-fluorescein combining sites in hyperimmunized rabbits: characterization by circular dichroism and binding studies
6. The tryptophan fluorescence lifetime puzzle A study of decay times in aqueous solution as a function of pH and buffer composition
7. On the contribution of tryptophan to the affinity and specificity of anti-dinitrophenyl antibodies
8. Partial elucidation of an anti-hapten repertoire in BALB/c mice: comparative characterization of several monoclonal anti-fluorescyl antibodies
9. Restricted reassociation of heavy and light chains from hapten-specific monoclonal antibodies
10. Evidence for tryptophan in the active sites of antibodies to polynitrobenzenes
11. The pH dependence of fluorescein fluorescence
12. Circular dichroism of hapten-antibody complexes: calculation of the interaction of a trinitrophenyl hapten with tryptophan
13. Performance characteristics of a small side-window photomultiplier in laser single-photon fluorescence decay measurements
14. First-order dissociation rates between a subpopulation of high-affinity rabbit anti-fluorescyl IgG antibody and homologous ligand
15. Mechanism of quenching of fluorescein by anti-fluorescein IgG antibodies
16. Spectral probe of an antibody active site