A new in vitro model for the study of pancreatic a and b cells

Endocrinology

Volumn 117, Issue 3, 1985, Pages 806-816

  Pipeleers, D G ^a   Van De Winkel, M ^a   Maes, E ^a   Schuit, F C ^a   In't Veld P A ^a   Gepts, W ^a  

^a VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

Author keywords

[No Author keywords available]

Indexed keywords

GLUCAGON; INSULIN;

ANIMAL CELL; DISSOCIATION; ENDOCRINE SYSTEM; IN VITRO STUDY; ISLET; ISOLATION; NONHUMAN; PANCREAS ISLET CELL; PRIORITY JOURNAL; RAT;

ANIMALS; CELL SEPARATION; DNA; FLAVIN-ADENINE DINUCLEOTIDE; FLOW CYTOMETRY; GLUCAGON; INSULIN; ISLETS OF LANGERHANS; MALE; METHODS; MICROSCOPY, ELECTRON; MICROSCOPY, ELECTRON, SCANNING; NADP; RATS; RATS, INBRED STRAINS; SOMATOSTATIN;

EID: 0021854805     PISSN: 00137227     EISSN: 19457170     Source Type: Journal    
DOI: 10.1210/endo-117-3-806     Document Type: Article

References (27)

1. Samols E, Tyler J, Kajinuma H 1970 Influence of the sulfonamides on pancreatic humoral secretion and evidence of an insulin-glucagon feedback system. Excerpta Medica Int Congress Series 231:636
2. Orci L, Unger RH 1975 Functional subdivision of islets of Langer-hans and possible role of D-cells. Lancet 2:1243
3. Orci L, Malaisse-Lagae F, Ravazzola M, Rouiller D, Renold AE, Perrelet A, Unger RH 1975 A morphological basis for intercellular communication between a-and β-cells in the endocrine pancreas. J Clin Invest 56:1066
4. Schauder E 1980 Somatostatin-endocrine or paracrine substance. Front Horm Res 7:52
5. Pipeleers DG, Pipeleers-Marichal MA 1981 A method for the purification of single A-, B-and D-cells and for the isolation of coupled cells from isolated rat islets. Diabetologia 20:654
6. Pipeleers D, Veld P, Maes E, Van De Winkel M 1982 Glucose-induced insulin release depends on functional cooperation between islet cells. Proc Natl Acad Sci USA 79:7322
7. Van De Winkel M, Smets G, Gepts W, Pipeleers D 1982 Islet cell surface antibodies from insulin-dependent diabetics bind specifically to pancreatic B-cells. J Clin Invest 70:41
8. Alejandro R, Shienvold FL, Hajek SV, Pierce M, Paul R, Mintz DH 1984 A ganglioside antigen on the rat pancreatic B-cell surface identified by monoclonal antibody R2D6. J Clin Invest 74:25
9. Russell TR, Noel J, Files N, Ingram M, Rabinovitch A 1984 Purification of beta cells from rat islets by monoclonal antibody-fluorescence flow cytometry. Cytometry 5:539
10. Nielsen DA, Lernmark A, Berelowitz M, Bloom GD, Steiner DF 1982 Sorting of pancreatic islet cell subpopulations by light scattering using a fluorescence-activated cell sorter. Diabetes 31:299
11. Rabinovitch A, Russell T, Shienvold F, Noel J, Files N, Patel Y, Ingram M 1982 Preparations of rat islet B-cell-enriched fractions by light scatter flow cytometry. Diabetes 31:939
12. Van De Winkel M, Maes E, Pipeleers D 1982 Islet cell analysis and purification by light scatter and autofluorescence. Biochem Biophys Res Commun 107:525
13. Van De Winkel M, Pipeleers D 1983 Autofluorescence-activated cell sorting of pancreatic islet cells: purification of insulin-containing B-cells according to glucose-induced changes in cellular redox state. Biochem Biophys Res Commun 114:835
14. Lacy PE, Kostianovsky M 1967 Method for the isolation of intact islets of Langerhans from the rat pancreas. Diabetes 16:35
15. Kuo WN, Hodgins DS, Kuo JF 1973 Adenylate cyclase in islets of Langerhans: isolation of islets and regulation of adenylate cyclase activity by various hormones and agents. J Biol Chem 248:2705
16. Pipeleers D, Somers G, Gepts W, De Nutte N, De Vroede M 1979 Plasma pancreatic hormone levels in a case of somatostinoma: diagnostic and therapeutic implications. J Clin Endocrinol Metab 49:572
17. Downs TR, Wilfinger WW 1983 Fluorometric quantification of DNA in cells and tissue. Anal Biochem 131:538
18. Gorus FK, Malaisse WJ, Pipeleers DG 1984 Differences in glucose handling by pancreatic A-and B-cells. J Biol Chem 259:1196
19. Maes E, Pipeleers D 1984 Effects of glucose and 3′,5′-cyclic adenosine monophosphate upon reaggregation of single pancreatic B-cells. Endocrinology 114:2205
20. Krause U, Puchinger H, Wacker A 1973 Inhibition of glucose-induced insulin secretion in trypsin-treated islets of Langerhans. Horm Metab Res 5:325
21. Kostianovsky M, McDaniel ML, Still MF, Codilla RC, Lacy PE 1974 Monolayer cell culture of adult rat islets of Langerhans. Diabetologia 10:337
22. Ono J, Takaki R, Fukuma M 1977 Preparation of single cells from pancreatic islets of adult rat by the use of dispase. Endocrinol Jpn 24:265
23. Weir GC, Halban PA, Meda P, Wollheim CB, Orci L, Renold AE 1984 Dispersed adult rat pancreatic islet cells in culture: A, B and D cell function. Metabolism 33:447
24. Baetens D, Malaisse-Lagae F, Perrelet A, Orci L 1979 Endocrine pancreas: three dimensional reconstruction shows two types of islets of Langerhans. Science 206:1323
25. Lernmark A 1974 The preparation of, and studies on, free cell suspensions from mouse pancreatic islets. Diabetologia 10:431
26. Buitrago A, Gylfe E, Henriksson C, Pertoft H 1977 Rapid isolation of pancreatic islets from collagenase digested pancreas by sedimentation through percoll™ at unit gravity. Biochem Biophys Res Commun 79:823
27. Offord RE, Halban PA 1978 Isolation of pancreatic islets of Langerhans by filtration on nylon mesh. Biochem Biophys Res Commun 82:1091