Direct Mitogenic Effects of Insulin, Epidermal Growth Factor, Glucocorticoid, Cholera Toxin, Unknown Pituitary Factors and Possibly Prolactin, but not Androgen, on Normal Rat Prostate Epithelial Cells in Serum-free, Primary Cell Culture

Cancer Research

Volumn 44, Issue 5, 1984, Pages 1998-2010

  McKeehan, Wallace L ^a   Adams, Pamela S ^a   Rosser, Mary P ^a  

^a Adirondack Biomedical Research Institute Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANDROGEN; ANDROSTANOLONE; CHOLERA TOXIN; DEXAMETHASONE; EPIDERMAL GROWTH FACTOR; GLUCOCORTICOID; HYPOPHYSIS EXTRACT; INSULIN; PROLACTIN;

ANIMAL CELL; ANIMAL EXPERIMENT; CELL CULTURE; ENDOCRINE SYSTEM; EPITHELIUM CELL; HYPOPHYSIS; IN VITRO STUDY; MALE GENITAL SYSTEM; NONHUMAN; PROSTATE; RAT;

ANDROGENS; ANIMAL; CELLS, CULTURED; CHOLERA TOXIN; CULTURE MEDIA; DEXAMETHASONE; EPIDERMAL GROWTH FACTOR; EPITHELIUM; INSULIN; MALE; MITOGENS; PITUITARY GLAND; PROLACTIN; PROSTATE; RATS; RATS, INBRED LEW; SUPPORT, U.S. GOV'T, P.H.S.; TISSUE EXTRACTS;

EID: 0021358147     PISSN: 00085472     EISSN: 15387445     Source Type: Journal    
DOI: None     Document Type: Article

References (62)

1. Aragona, C., and Friesen, H. G. Specific prolactin binding sites in prostate and testis of rats. Endocrinology, 97: 677–684, 1975.
2. Asano, M., Kanzaki, S., Sekiguchi, E., and Tasaka, T. Inhibition of prostatic growth in rabbits with antiovíne prolactin serum. J. Urol., 106:248–252, 1971.
3. Barnes, D., and Sato, G. Methods for growth of cultured cells in serum-free medium. Anal. Biochem., 702: 255–270, 1980.
4. Bauelieu, E. E., LeGoascogne, C, Groyer, A., Fezel-Cabanes, T., and Robel, P. Morphological and biochemical pararneters of androgen effects on rat ventral prostate in organ culture. Vrtam. Horm., 33:1–38, 1975.
5. Bettger, W. J., Boyce, S. T., Walthall, B. J., and Ham, R. G. Rapid clonal growth and serial passage of human diploid fibroblasts in a lipid enriched synthetic medium supplemented with epidermal growth factor, insulin, and dexamethasone. Proc. Natl. Acad. Sci. USA, 78: 5588–5592.1981.
6. Boileau. M., Keenan, E., Kemp, E., Lawson, R., and Hodges, C. V. Effect of human serum on thymidine H-3 incorporation in human prostate tumors in tissue culture. J. Urol., 119: 777–779, 1978.
7. Carpenter, G. Epidermal growth factor. In: Handbook of Experimental Pharmacology, Vol. 57, pp. 89–132. Berlin: Springer-Veriag, 1981.
8. Chaproniere-Rickenberg, D. M., and Webber, M. M. A chemically defined media for the growth of adult human prostate epithelium. In: G. H. Sato, A. B. Pardee, and D. A. Sirbasku (eds.), Growth of Cells in Hormonaily Defined Media, Vol. 9, pp. 1109–1115. Cold Spring Harbor, NY: Cokj Spring Harbor Laboratory, 1982.
9. Chase, M. D., Geschwind, I.I., and Bern, H. A. Synergistic role of prolactin in response of male rat sex accessories to androgen. Proc. Soc. Exp. Biol. Med., 94: 680–683, 1957.
10. Chevalier, S., Bleau, G., Roberts, K. D., and Chapdelaine, A. Proliferation and differentiation of canine prostatic epithelial cells in culture. Mol. Cell. Endocrinol., 24:195–208, 1981.
11. Clark, A. F., Ortowski, J., and Bird, C. E. Culture of immature rat prostate epithelial cells (abstract). J. Androl., 3:17, 1982.
12. Ctemmons, D. R., and Van Wyk, J. J. Somatomedin: physiological control and effects on cell proliferation. In: Handbook of Experimental Pharmacology, Vol. 57, pp. 161–208. Berlin: Springer-Veriag, 1981.
13. Cristofak), V. J., and Rosner, B. A. Glucocorticoid modulation of ceH proliferation. In: Handbook of Experimental Pharmacology, Vol. 57, pp. 209–228. Berlin: Springer-Veriag, 1981.
14. Epstein, M., Ross, E. M., DeWotf. M. J. S., Fridkin, M., and Kohn, L. D. Glycoprotein hormone—cholera toxin relationships: implications for role of ADP-ribosytation in cyclase activation. In: T. Smuteon and M. Sugimura (eds.), Novel ADP-Ribosylations of Regulatory Enzymes and Proteins, pp. 369–379. Amsterdam: Elsevier/North-Holland Biomedical Press, Inc., 1980.
15. Famsworth, W. E. Prolactin and the prostate. In: A. R. Boyns and K. Griffith (eds.), Prolactin and Carcinogenesis (Fourth Tenovirus Workshop), pp. 217–225. Cardiff, Wales: Alpha Omega Alpha, 1972.
16. Franke, W. W., Appelhaus, B., Schmid, E., Freudenstein, C, Osbom, M., and Weber, K. Identification and characterization of epithelial cells in mammalian tissues by immunofluorescence microscopy using antibodies to prekeratin. Differentiation, 15:17–25, 1979.
17. Gospodarowicz, D., Delgado, D., and Vladovsky, I. Permissive effect of the extracellular matrix on cell proliferation in vitro. Proc. Natl. Acad. Sci. USA, 77: 4094–4098, 1980.
18. Grayhack, J. T. Pituitary factors influencing growth of the prostate. Natl. Cancer Inst. Monogr., 12:189–199, 1963.
19. Green, H. Cycfic-AMP in relation to proliferation of epidermal ceH—new view. Cell, 75:801–811, 1978.
20. Ham, R. G., and McKeehan, W. L Media for growth of cells in culture. Methods Enzymol., 59:44–93, 1979.
21. Heyns, B., Peeters, B., Mous, J., Rombauts, W., and De Moor, P. Androgen dependent synthesis of a prostatic binding protein by rat prostate. J. Steroid Biochem., 11:209–213, 1979.
22. Hoisaeter, P. A., Kadohama, N., Corrates, J. J., Karr, J. P., Murphy, G. P., and Sandberg, A. A. Characterization of androgen receptor and estramustine binding protein of rat ventral prostatic tissue in organ culture. J. Steroid Biochem., 14: 251–260, 1981.
23. Huggins, C., and Russell, P. S. Quantitative effects of hypophysectomy on testis and prostate of dog. Endocrinology, 39: 1–7, 1946.
24. Keenen, E. J., and Thomas, J. A. Effects of testosterone and prolactin or growth hormone on accessory sex organs of castrated mice. J. Endocrinol., 64:111–115, 1975.
25. King, G. L., Kahn, C. R., Reenter, M. M., and Nissley. S. P. Direct demonstration of separate receptors for growth and metabolic activities of insulin and multiplication stimulating activity (an insulin-like growth factor) using antibodies to the insulin receptor. J. Clin. Invest., 66:130–140, 1980.
26. Kledzik, G. S., Marshall, S., Campbell, G. A., Gelato, M., and Meites, J. Effects of castration, testosterone, estradiol, and prolactin on specific proiactin-binding activity in ventral prostate of male rats. Endocrinology, 98: 373–379, 1976.
27. Kubota, Y., Gehly, E. B., Link, K. H., and Hektelberger, C. Development of cloned epithelial cell lines from normal adult mouse and rat ventral prostates. In Vitro (Rockville), 77: 965–978, 1981.
28. Lasnitski, I. Action and interaction of hormones and 3-methylcholanthrene on ventral prostate gland of rat in vitro. I. Testosterone and methyteholanthrene. J. Natl. Cancer Inst., 35: 339–348, 1965.
29. Laugier, C, Pageaux, J. F., Soto, A. M., and Sonnenschein, C. Mechanism of estrogen action: indirect effect of estradioJ-17/5 on proliferation of quail oviduct cells. Proc. Natl. Acad. Sci. USA, 80:1621–1625, 1983.
30. Lechner, J. F., Haugen, A., Autrup, H., McClendon, I. A., Trump, B. F., and Harris, C. C. Clonal growth of epithelial cells from normal adult human bronchus. Cancer Res., 41:2294–2304, 1981.
31. Lechner, J. F., and Kaighn, M. E. Nutrition of prostate cells. In: G. P. Murphy (ed.), Models for Prostate Cancer, pp. 217–232. New York: Alan R. Liss, Inc., 1980.
32. Lesser, B., and Bruchovsky, N. Effect of duration of period after castration on response of rat ventral prostate to androgens. Biochem. J., 142: 429–431, 1974.
33. Lewis, R. W., and Kaack, B. Nonhuman primate prostate culture. In: G. P. Murphy (ed.), Models for Prostate Cancer, pp. 39–65. New York: Alan R. üss, Inc., 1980.
34. Lostroh, A. J. Effect of testosterone and growth hormone on nucleic acid and protein in the sex accessory glands of Long-Evans and Sprague-Dawley rats. Endocrinology, 70: 742–746.1962.
35. McKeehan, W. L., Adams, P. S., and Rosser, M. P. Modified nutrient Medium MCDB151 (WAJC 401), defined growth factors, cholera toxin, pituitary factors, and horse serum support epithelial ceH and suppress fibroblast proliferation in primary cultures of rat ventral prostate cells. In Vitro (Rockville), 18: 87–91, 1982.
36. McKeehan, W. L., Glass, H. A., Rosser, M. P., and Adams, P. S. Prostatic binding protein, poly amine, and DNA synthesis in rat ventral prostate cells. Prostate, 3: 231–246.1982.
37. McKeehan, W. L., McKeehan, K. A., and Calkins, D. Extracellular regulation of fibroblast multiplication—quantitative differences in nutrient and serum factor requirements for multiplication of normal and SV40 virus transformed human lung cells. J. Biol. Chem., 256: 2973–2981, 1981.
38. McKeehan, W. L., McKeehan, K. A., Hammond, S. L., and Ham, R. G. Improved medium for clonal growth of human diploid cells at low concentrations of serum protein. In Vitro (Rockville), 73: 399–416, 1977.
39. McKeehan, W. L., Rosser, M. P., Glass, H. A., and Fast, D. Prostatic binding protein—an androgen dependent marker for prostate epithelial cells. Biochem. Biophys. Res. Commun., 95:674–681, 1980.
40. Moss, J., and Vaughn, M. Activation of adenylate cyclase by choieragen. Annu. Rev. Biochem. 48: 581–600, 1979.
41. Page, M. J., and Parker, M. G. Androgen-regulated expression of a cloned rat prostate C3-gene transfected into mouse mammary tumor cells. Cell, 32:495502, 1983.
42. Parker, M. G., Scrace, G. T., and Main waring, W. I. P. Testosterone regulates synthesis of major proteins in rat ventral prostate. Biochem. J., 770:115–121, 1978.
43. PeeN, D. M., and Ham, R. G. Clonal growth of human keratinocytes with small amounts of dialyzed serum. In Vitro (Rockville), 76:526–540, 1980.
44. Pousette, A., Bjork, P., Caristrom, K., Forsgren, B., Hogberg, B., and Gustafs-son, J.-A. Influence of sex hormones on prostate secretion protein: a major protein in rat prostate. Cancer Res., 41:688–690, 1981.
45. Pousette, A., Bjork, P., Caristrom, K., Forsgren, B., Hogberg, B., and Gustafs-son, J. A. Influence of pituitary and adrenocortical hormones on prostate secretion protein, a major protein in rat prostate. Prostate, 3:109–114, 1982.
46. Sanford, E. J., Geder, L, Jones, R. E., Rohner, T. J., and Rapp, F. In vitro cultures of human prostatic tissue. Urol. Res., 5: 207–210, 1977.
47. Schlegel, R., Banks-Schtegel, S., and Pinkus, G. D. Immunohistochemical localization of keratin in normal human tissues. Lab. Invest., 42:91–96.1980.
48. Schroeder, F. H., and Mackensen, S. J. Human prostatic adenoma and carcinoma in cell culture—effects of androgen-free culture medium. Invest. Urol., 72:176–181, 1974.
49. Sestili, M. A., Norris, J. S., and Smith, R. G. Isolation and characterization of a cloned cell line R3327H-G8-A1 derived from the Dunning R3327H rat adenocarcinoma. Cancer Res., 43:2167–2175, 1983.
50. Shain, S. A., Boesei, R. W., Kalter, S. S., and Heberiing, R. L. AXC rat prostatic adenocarcinoma. Characterization of cells in culture. In: W. W. Leavitt (ed.), Hormones and Cancer, pp. 337–351. New York: Plenum Publishing Corp., 1982.
51. Sirbasku, D. A. Estrogen induction of growth factors specific for hormone responsive mammary, pituitary, and kidney tumor cells. Proc. Natl. Acad. Sci. USA, 75: 3786–3790, 1978.
52. SlaunwNte, W. R., and Sharma, M. Effects of hypophysectomy and prolactin replacement therapy on prostatic response to androgen in orchiectomized rats. Biol. Reprod., 17:489–492, 1977.
53. Sun, T. T., and Green, H. Keratin filaments of cultured human epidermal cells— formation of intermotecuiar disufide bonds during terminal differentiation. J. Biol. Chem., 253: 2053–2060.1978.
54. Sun, T. T., and Green, H. Keratin cytosketetons in epithelial cells of internal organs. Proc. Natl. Acad. Sci. USA., 76:2813–2817, 1979.
55. Syms, A. J., Harper, H. E., Battersby, S., and Griffiths, K. Proliferation of human prostatic epithelial cells in culture—aspects of identification. J. Urol., 727:561–567, 1982.
56. Taylor-Papadirnitríou, J., Purkis, P., and Fentiman, I. S. Cholera toxin and analogs of cycüc-AMP stimulate the growth of cutured human mammary epithelial cells. J. Cell. Physiol., 702; 317–321, 1980.
57. Tsao, M. C., Walthall, B. J., and Ham, R. G. Clonal growth of normal human epidermal keratinocytes in a defined medium. J. CeN Phystol., 110:219–229, 1982.
58. Tullner, W. W. Hormonal factors in the adrenal dependent growth of the rat ventral prostate. Natl. Cancer Inst. Monogr., 12:211–220, 1968.
59. Tymoczko, J. L., Liang, T., and Uao, S. Androgen receptor interactions in target cells: biochemical evaluation. In: B. W. O'Malley and L. Bimbaumer (eds.), Receptors and Hormone Action, Vol. 2, pp. 121–156. New York: Academic Press, Inc., 1978.
60. Vanderiaan, W. P. Observations on the hormonal control of the prostate. Lab. Invest., 9:185–190, 1960.
61. Witorsch, R. J., and Smith, J. P. Evidence for arrirogen-dependent intracellular binding of prolactin in rat ventral prostate gland. Endocrinology, 101: 929–938 1977.
62. Worton, R. G., and Duff, C. Karyotyping. Methods Enzymol., 59: 322–344, 1979.