Isolation, Culture and Characterization of Adult Human Hepatocytes from Surgical Liver Biopsies

Hepatology

Volumn 4, Issue 5, 1984, Pages 849-854

  Ballet, Francois ^a   Bouma, Marie‐Elisabeth ^a   Wang, Shu‐Ren ^a   Amit, Norma ^a   Marais, Jacqueline ^a   Infante, Recaredo ^a  

^a HÔPITAL SAINT ANTOINE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

ADULT; CELL ISOLATION; CYTOLOGY; DIAGNOSIS; ELECTRON MICROSCOPY; HISTOCHEMISTRY; HISTOLOGY; HUMAN; HUMAN CELL; LIVER; LIVER BIOPSY; LIVER CELL; LIVER CELL CULTURE; MORPHOLOGY; ULTRASTRUCTURE;

ADULT; ALBUMINS; BILE CANALICULI; BIOPSY; CELL SURVIVAL; CELLS, CULTURED; FEMALE; GLUCOSE-6-PHOSPHATASE; HUMAN; IMMUNOENZYME TECHNIQUES; LIPIDS; LIVER; MALE; MICROSCOPY, ELECTRON; MICROSCOPY, PHASE-CONTRAST; MIDDLE AGE; PERFUSION; TIME FACTORS;

EID: 0021201874     PISSN: 02709139     EISSN: 15273350     Source Type: Journal    
DOI: 10.1002/hep.1840040509     Document Type: Article

References (32)

1. High yield preparation of isolated rat liver parenchymal cells. A biochemical and fine structural study
2. The enzymatic preparation of isolated intact parenchymal cells from rat liver
3. Primary culture and the level of cytochrome P 450 in hepatocytes from mouse, rat, hamster and rabbit liver
4. La culture prolongée du tissu hépatique humain adulte
5. Tissue culture of adult human liver
6. An ultrastructural study of primary cultures of adult human liver tissue
7. Maintenance in vitro of functionally active adult human liver
8. Comparative study of extracellular aminoacids in culture of human liver and fibroblastic cells
9. Kinetics of nuclear changes during herpetic infection of human primary liver cell cultures
10. Culture prolongee d'hepatocytes provenant de foie sain de sujets humains adultes; caracterisation immunologique de la nature hepatocytaire des cellules cultivees
11. Production of albumin and other serum proteins by clonal cultures of normal human liver
12. Lymphocyte cytotoxicity to autologous liver cells in chronic active hepatitis
13. Studies on the pathogenesis of chronic inflammatory liver diseases. I. Membrane‐fixed IgG on isolated hepatocytes from patients. Clin
14. An improved method for the isolation of hepatocytes from liver slices
15. Isolation and primary culture of adult human hepatocytes. Ultrastructural and functional studies
16. Preparation of human hepatocytes for primary culture
17. Isolation and culture of adult hepatocytes from liver biopsies
18. Isolation, culture and transplantation of human hepatocytes
19. Preparation of rat liver cells. II. Effects of ions and chelators on tissue dispersion
20. Etude ultrastructurale de monocouches d'hepatocytes de rat adulte cultives en presence d'hemisuccinate d'hydrocortisone
21. Haematoxylins
22. Azure C as tissue stain
23. The distribution of glucose‐6‐phosphatase in the liver and in the kidney of the mouse
24. A method for the in situ embedding of cultured cells grown on plastic surfaces
25. Indirect immunoenzyme techniques for the intracellular detection of antigens
26. The early stage of absorption of injected horseradish peroxidase in the proximal tubule of mouse kidney: ultrastructural cytochemistry by a new technique
27. Quantitative estimation of proteins by electrophoresis on agarose gel containing antibodies
28. Preparation of parenchymal and non‐parenchymal cells from adult human liver. Morphological and biochemical characteristics
29. High yield preparation of isolated human adult hepatocytes by enzymatic perfusion of the liver
30. Functional integrity of isolated rat hepatocytes prepared by whole liver vs. biopsy perfusion
31. The perfused human liver wedge biopsy: a new in vitro model for morphological and functional studies