Construction of improved M13 vectors using oligodeoxynucleotide-directed mutagenesis

Gene

Volumn 26, Issue 1, 1983, Pages 101-106

  Norrander, Jan ^a   Kempe, Tomas ^b   Messing, Joachim ^a  

^a UNIVERSITY OF MINNESOTA   (United States)

^b Molecular Genetics Inc   (United States)

Author keywords

DNA polymerase I Klenow fragment; gene synthesis; M13 cloning; phosphoramidites; synthetic DNA primers

Indexed keywords

RADIOISOTOPE;

BACTERIOPHAGE; CLONING VECTOR; GENETIC ENGINEERING; HEREDITY; MUTAGENESIS; NONHUMAN;

BASE SEQUENCE; CLONING, MOLECULAR; COLIPHAGES; COMPARATIVE STUDY; DNA RESTRICTION ENZYMES; GENETIC VECTORS; MUTATION; OLIGODEOXYRIBONUCLEOTIDES; OLIGONUCLEOTIDES; SUPPORT, U.S. GOV'T, NON-P.H.S.; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0021073779     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(83)90040-9     Document Type: Article

References (23)

1. Deoxynucleoside phosphoramidites — a new class of key intermediates for deoxypolynucleotide synthesis
2. A membrane-filter technique for the detection of complementary DNA
3. Site-specific mulagenesis using synthetic oligodeoxyribonucleotidc primers: I. Optimum conditions and minimum oligodeoxyribonucleotide length
4. Site-specific mutagenesis using oligodeoxyribonucleotides: isolation of a phenotypically silent φX174 mutant, with a specific nucleotide deletion, at very high efficiency
5. Methylation of single-stranded DNA in vitro introduces new restriction endonuclease cleavage sites
6. Mutagenesis at a specific position in a DNA sequence
7. Selective 2'-benzoylation at the cis 2'. 3'-diols of protected ribonucleosides. New solid phase synthesis of RNA and DNA-RNA mixtures
8. New versatile cloning and sequencing vectors based on bacteriophage M13
9. Apple II software for M 13 shotgun DNA sequencing
10. Apple II computer software for DNA and protein sequence data
11. Filamentous coliphage M13 as a cloning vehicle: Insertion of a HindIII fragment of the lac regulatory region in the M 13 replicative form in vitro
12. A multipurpose cloning system based on the single-stranded DNA bacteriophage M 13. Recombinant DNA
13. A system for shotgun DNA sequencing
14. M13mp2 and derivatives: A molecular cloning system for DNA sequencing, strand specific hybridization. and in vitro mutagenesis
15. A new pair of M 13 vectors for selecting either DNA strand of double-digest restriction fragments
16. An integrative strategy of DNA sequencing and experiments beyond
17. In vitro DNA synthesis as a tool to analyze and alter genes
18. New M 13 vectors for cloning
19. The manipulation of zein genes to improve the nutritional value of corn
20. DNA sequencing with chain-terminating inhibitors
21. Directed deletion of a yeast transfer RNA intervening sequence
22. Oligonucleotide-directed mutagenesis using M13-derived vectors; an efficient and general procedure for the production of point mutations in any fragment of DNA
23. Oligonucleotide-directed mutagenesis of DNA fragments cloned into M 13 vectors