Vectors bearing a hybrid trp-lac promoter useful for regulated expression of cloned genes in Escherichia coli

Gene

Volumn 25, Issue 2-3, 1983, Pages 167-178

  Amann, Egon ^a   Brosius, Jürgen ^a   Ptashne, Mark ^a  

^a HARVARD UNIVERSITY   (United States)

Author keywords

cI repressor overproduction; eukaryotic protein overproduction; pBR322; Recombinant DNA

Indexed keywords

LACTOSE; TRYPTOPHAN;

CELL HYBRIDIZATION; CLONING VECTOR; ESCHERICHIA COLI; EXTENSION GENE; GENE EXPRESSION; GENETIC ENGINEERING; HEREDITY; MOLECULAR CLONING; NONHUMAN;

BACTERIAL PROTEINS; BASE SEQUENCE; CLONING, MOLECULAR; DNA, BACTERIAL; ESCHERICHIA COLI; GENE EXPRESSION REGULATION; LAC OPERON; PLASMIDS; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0021019879     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(83)90222-6     Document Type: Article

References (29)

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12. Starting and stopping sequences for the RNA polymerase
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14. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
15. Construction of overproducers of the bacteriophage 434 repressor and cro proteins
16. A lac promoter with a changed distance between −10 and −35 regions
17. Calcium-dependent bacteriophage DNA infection
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19. Sequencing end-labeled DNA with base-specific chemical cleavages
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21. Regulatory sequences involved in the promotion and termination of RNA transcription
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23. Nucleotide sequence of an RNA polymerase binding site from the DNA of bacteriophage fd
24. E. coli RNA polymerase interacts homologously with two different promoters
25. s promoter
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27. Construction of plasmid vectors with unique PstI cloning sites in a signal sequence coding region
28. Expression of the human fibroblast interferon gene in E. coli
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