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Volumn 220, Issue 4597, 1983, Pages 611-613

Heparan sulfate degradation: Relation to tumor invasive and metastatic properties of mouse B16 melanoma sublines

Author keywords

[No Author keywords available]

Indexed keywords

ENDOGLYCOSIDASE; HEPARAN; UNCLASSIFIED DRUG;

EID: 0020570408     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.6220468     Document Type: Article
Times cited : (306)

References (33)
  • 23
    • 0017346890 scopus 로고
    • B16 melanoma sublines were grown to subcon-fluence in a 1:1 mixture of Dulbecco-modified Eagle's and F12 medium supplemented with 5 percent heat-inactivated fetal bovine serum (Re-heis Chemical Co.) and 1 percent nonessential amino acids without antibiotics. Cells were rinsed twice in medium without serum, detached in calcium- and magnesium-free phosphate-buffered saline containing 2 mM EDTA, and rinsed again in medium without serum before use. The cells were used within ten passages from frozen stocks and were found to be free from mycoplasma Contamination
    • (1977) Exp. Cell Res. , vol.104 , pp. 255
    • Chen, T.R.1
  • 26
    • 85027781934 scopus 로고
    • Purified heparan sulfate from bovine lung (50 μ g) was incubated with each cell homogenate (40 μg of protein) or a boiled cell homogenate in 70 μl of 0.1M sodium phosphate, 0.2 percent Triton X-100, 0.15M sodium chloride, pH 6.0 at 37°C for various times. After incubation, the mixtures were centrifuged to remove debris and 2 μl of the super- natants were applied to 6 percent polyacryl-amide gels in 50 mM 1,3-diaminopropane acetate buffer, pH 9.0. Electrophoresis was per formed at 120 V for 60 minutes at 4°C. Electrophoresed gels were fixed and then stained with 0.1 percent toluidine blue in 1 percent acetic acid. Sulfated glycosaminoglycans larger than octasaccharides can be stained and scanned at 525 nm for quantification
    • (1976) Anal. Biochem. , vol.70 , pp. é45
    • Dietrich, C.P.1    Dietrich, S.M.C.2


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.