Ethidium-bromide-inhibited restriction endonucleases cleave one strand of circular DNA

Gene

Volumn 20, Issue 1, 1982, Pages 121-125

  Österlund, Mårten ^a   Luthman, Holger ^a   Nilsson, Stefan V ^a   Magnusson, Göran ^a  

^a KAROLINSKA INSTITUTE   (Sweden)

Author keywords

increased dye binding of cleavage intermediates; Intercalation; plasmid pBR322; stabilization of cleavage intermediates

Indexed keywords

DNA; ETHIDIUM BROMIDE; RESTRICTION ENDONUCLEASE;

GENETIC ENGINEERING; NONHUMAN; R PLASMID; TEMPERATURE;

COMPARATIVE STUDY; DNA RESTRICTION ENZYMES; DNA, CIRCULAR; DNA, SINGLE-STRANDED; ETHIDIUM; PLASMIDS; SUBSTRATE SPECIFICITY; SUPPORT, NON-U.S. GOV'T;

EID: 0020432796     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(82)90093-2     Document Type: Article

References (11)

1. The interaction of closed circular DNA with intercalative dyes I. The superhelix density of SV40 DNA in the presence and absence of dye
2. Construction and characterization of new cloning vehicles, II. A multi-purpose cloning system
3. Conversion of closed circular DNA molecules to single-nicked molecules by digestion with DNase I in the presence of ethidium bromide
4. Transient cleavage kinetics of the EcoRI restriction endonuclease measured in a pulsed quench-flow apparatus: enzyme concentration-dependent activity change
5. EcoRI endonuclease. Physical and catalytic properties of the homogenous enzyme
6. Sealing of gaps in duplex DNA by T4 DNA ligase
7. Mapping of closed circular DNAs by cleavage with restriction endonucleases and calibration by agarose gel electrophoresis
8. Construction of a specific amber codon in the simian virus 40 T-antigen gene by site-directed mutagenesis
9. Restriction and modification enzymes and their recognition sequences
10. Local mutagenesis: A method for generating viral mutants with base substitutions in pre-selected regions of the viral genome
11. Complex formation between ethidium bromide and nucleic acids