Calculation of free and bound fractions of testosterone and estradiol-17β to human plasma proteins at body temperature

Journal of Steroid Biochemistry

Volumn 16, Issue 6, 1982, Pages 801-810

  Södergard, Ragnar ^a   Bäckström, Torbjörn ^a   Shanbhag, Vasant ^a   Carstensen, Hans ^a  

^a UMEÅ UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ALBUMIN; ANDROSTANOLONE; ESTRADIOL; PLASMA PROTEIN; SEX HORMONE BINDING PROTEIN; TESTOSTERONE;

BLOOD AND HEMOPOIETIC SYSTEM; FEMALE GENITAL SYSTEM; HUMAN; HUMAN CELL; HUMAN EXPERIMENT; MALE GENITAL SYSTEM;

ADULT; BODY TEMPERATURE; ESTRADIOL; FEMALE; HUMAN; MALE; MATHEMATICS; MIDDLE AGE; PROTEIN BINDING; SERUM ALBUMIN; SEX FACTORS; SEX HORMONE-BINDING GLOBULIN; STANOLONE; SUPPORT, NON-U.S. GOV'T; TESTOSTERONE;

EID: 0020417830     PISSN: 00224731     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-4731(82)90038-3     Document Type: Article

References (49)

1. Transcortin, a corticosteroid-binding protein of plasma
2. Protein-steroid interactions and their role in the transport and metabolism of steroids
3. Steroid protein interactions
4. Interactions between steroids and proteins
5. The apparent free testosterone concentration, an index of androgenicity
6. The measurement of sex steroid binding globulin by differential ammonium sulphate precipitation
7. Sex-hormone-binding globulin is an oestrogen amplifier
8. Accurate measurement of steroid-protein binding by steady-state gel filtration
9. Plasma dihydrotestosterone in normal adult males and its relation to testosterone
10. Methods for multiple steroid radioimmunoassay
11. Androstenediol levels in human peripheral plasma
12. 3α-Androstanediol in human peripheral plasma
13. Measurement of plasma testosterone by means of competitive protein binding analysis
14. Binding of 5α-androstane-3α,17β-diol to human plasma proteins
15. Methods of paper chromatography of steroids applicable to the study of steroids in mammalian blood and tissues
16. A paper chromatographic saturation analysis method for measuring estradiol, testosterone and 5α-dihydrotestosterone from the same sample
17. A new method for the determination of the binding capacity of testosterone-estradiol-binding globulin in human plasma
18. The attractions of proteins for small molecules and ions
19. Direct spectrophotometric determination of albumin in human serum
20. Study of steroid-protein binding by means of competitive adsorption: application to cortisol binding in plasma
21. A graphic method for the determination and presentation of binding parameters in a complex system
22. Steroid-protein interactions
23. Sex steroid binding plasma protein (SBP)
24. Multiple equilibria in proteins
25. Capacity of the testosterone-binding globulin in human plasma and influence of specific binding of testosterone on its metabolic clearance rate
26. Testosterone-binding levels in the serum of women during the normal menstrual cycle, pregnancy and the postpartum period
27. Isolation and characterization of the testosterone-estradiol-binding globulin from human plasma. Use of a novel affinity column
28. A further study of a testosterone-binding component of human pregnancy serum
29. Studies on the binding of testosterone to human plasma
30. Steroids and the practical aspects of performing studies
31. Relative binding of testosterone and estradiol to testosterone-estradiol-binding globulin
32. Free and protein-bound plasma estradiol-17β during the menstrual cycle
33. Plasma concentration of free and non-TeBG bound testosterone in women on oral contraceptives
34. Status of estrogen-androgen balance in hyperthyroid men with Grave's disease
35. Testosterone, androstenedione and dehydroepiandrosterone in plasma during pregnancy and at delivery; concentration and protein binding
36. Secretion rates of androgens in human subjects
37. 3H]-testosterone metabolism in normal young men; effects of medroxyprogesterone acetate (Provera) administration
38. Further studies on Leydig cell function in old age
39. Elevated free testosterone in the plasma of early pregnant women bearing male fetuses and of hypersexual men
40. Alterations in circulating estradiol-17β in male patients with Grave's disease
41. Concentration of estradiol, testosterone and progesterone in cerebrospinal fluid compared to plasma unbound and total concentrations
42. Calcium, phosphate and albumin in serum
43. Studies of the protein binding of dihydrotestosterone in human plasma (In different physiological conditions and effect of medroxyprogesterone)
44. An assay for sex hormone binding globulin based on affinity chromatography
45. Steroid-protein interaction with particular reference to testosterone binding by human serum