Flow cytometric deoxyribonucleic acid analysis of granulosa cells aspirated from human ovarian follicles. a new method to distinguish healthy and atretic ovarian follicles

Journal of Clinical Endocrinology and Metabolism

Volumn 55, Issue 4, 1982, Pages 693-698

  Westergaard, Lars ^a   McNatty, Kenneth P ^b   Christensen, I ^c   Larsen, Jørgen K ^c   Byskov, Anne Grete ^c  

^a HVIDOVRE HOSPITAL   (Denmark)

^b LEIDEN UNIVERSITY MEDICAL CENTER   (Netherlands)

^c FINSEN LABORATORY   (Denmark)

Author keywords

[No Author keywords available]

Indexed keywords

DNA;

CYTOLOGY; DIAGNOSIS; ENDOCRINE SYSTEM; FEMALE GENITAL SYSTEM; FLOW CYTOMETRY; HUMAN; HUMAN CELL; METHODOLOGY; NORMAL HUMAN; OVARY FOLLICLE ATRESIA; OVARY FOLLICLE STRATUM GRANULOSUM; PRELIMINARY COMMUNICATION;

ADOLESCENT; ADULT; ANDROSTENEDIONE; BODY FLUIDS; DNA; ESTRADIOL; FEMALE; FLOW CYTOMETRY; GRANULOSA CELLS; HUMANS; MITOSIS; OVARIAN FOLLICLE;

EID: 0020402641     PISSN: 0021972X     EISSN: 19457197     Source Type: Journal    
DOI: 10.1210/jcem-55-4-693     Document Type: Article

References (17)

1. Block E 1951 Qualitative morphological investigations of the follicular system in women. Acta Endocrinol (Copenh) 8: 33
2. McNatty KP, Moore Smith D, Makirs A, Osathanondh R, Ryan KJ 1979 The microenvironment of the human antral follicle: Interrelationships among the steroid levels in antral fluid, the populationof granulosa cells, and the status of oocyte in vivo and in vitro. J Clin Endocrinol Metab 49: 851
3. Bomsel-Helmreich O, Gougeon A, Thebault A, Saltarelli D, Milgrom E, Frydman R, Papiernik E 1979 Healthy and atretic follicles in the preovulatory phase: Differences in the evolution of follicular morphology and the steroid content of follicular fluid. J Clin Endocrinol Metab 48: 686
4. Brailly S, Gougeon A, Milgrom E, Bomsel-Helmreich O, Papiernik E 1981 Androgens and progestins in the human ovarian follicles: Differences in the evolution of preovulatory, healthy nonovulatory, and atretic follicles. J Clin Endocrinol Metab 53: 128
5. Hillier SG, Reicher Jr LE, van Hall E 1981 Control of preovulatory follicular estrogen biosynthesis in the human ovary. J Clin Endocrinol Metab 52: 847
6. McNatty KP, Ovarian follicular development from the onset of luteal regression in humans and sheep. In: R Roland, E. V. van Hall, S. G. Hillier, K. P. McNatty, S. Schoemaker (ed): Follicular Maturation and Ovulation. Proceedings of the IVth Reinier de Graaf Symposium. Nijmegen, August 20-22,1981, Excerpta Medica, Amsterdam, 1982, pp 1-18
7. Vindeløv LL, Hansen HH, Christensen I, Spang-Thomsen M, Hirsch FR, Hansen M, Nissen NI 1980 Clonal heterogenity of small-cell anaplastic carcinoma of the lung demonstrated by flowcytometric DNA analysis. Cancer Res 40: 4295
8. Vindeløv LL 1977 Flow microfluorometric analysis of nuclear DNA in cells from solid tumors and cell suspensions. Virchows Arch B Cell Pathol 24: 227
9. Christensen I, Hartmann NR, Keiding N, Larsen JK, Noer H, Vindeløv LL 1978 Statistical analysis of DNA distributions from cell populations with partial synchrony. In: Lutz D (ed) Pulse Cytophotometry III. European Press, Ghent, p 71
10. Frentz G, Møller U, Christensen I 1980 DNA flow cytometry on human epidermis: I. Methodological studies on normal skin. J Invest Dermatol 74: 119
11. Delforge JP, Thomas K, Roux F, Carneiro de Siqueira J, Ferin J 1972 Time relationships between granulosa cells growth and luteinization and plasma luteinizing hormone discharge in human. I. A morphometric analysis. Fertil Steril 23: 1
12. Hillier SG, Brownsey BG, Cameron EHD 1973 Some observations on the determination of testosterone in human plasma by radioimmunoassay using antisera raised against testosterone-3 BSA and testosterone-11-BSA. Steroids 21: 735
13. Hillier SG, van den Boogaard AMJ, Reichert Jr LE, van Hall EV 1980 Alterations in granulosa cell aromatase activity accompanying preovulatory follicular development in the rat ovary with evidence that 5 alpha-reduced C19 steroids inhibit the aromatase reaction in vitro. J Endocrinol 84: 409
14. Hillier SG, Knazek RA, Ross GT 1977 Androgenic stimulation of progesterone production by granulosa cells from preantral ovarian follicles: Further in vitro studies using replicate cell cultures. Endocrinology 100: 1539
15. McNatty KP, Baird DT 1978 Relationships between follicle-stimulating hormone, androstenedione and oestradiol in human follicular fluid. J Endocrinol 76: 527
16. McNatty KP 1980 Ovarian regulation of follicle maturation in the human ovary: A role for 5a-reduced androgens. In: Cumming IA, Funder JW, Mendelsohn FAO (eds) Endocrinology 1980. Australian Academy of Science, Canberra City, pp 420-424
17. Coulson PB 1979 Characterization of polyploidy in ovarian granulosa cells. In: Midgley AR, Sadler WA (eds) Ovarian Follicular Development and Function. Raven Press, New York, pp 385-394