Lethality of palindromic DNA and its use in selection of recombinant plasmids

Gene

Volumn 19, Issue 1, 1982, Pages 147-151

  Hagan, Carole E ^a   Warren, Gareth J ^a  

^a UNIVERSITY OF DUNDEE   (United Kingdom)

Author keywords

ColE1 replicons; Escherichia coli K 12; Inverted repeat sequences; molecular cloning; recombinant DNA; transposon Tn 5

Indexed keywords

RECOMBINANT DNA;

ESCHERICHIA COLI; GENETIC ENGINEERING; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; TRANSPOSON;

BASE SEQUENCE; CLONING, MOLECULAR; DEOXYRIBONUCLEASE HINDIII; DNA RESTRICTION ENZYMES; DNA, RECOMBINANT; ESCHERICHIA COLI; GENES, LETHAL; MOLECULAR WEIGHT; PLASMIDS; REPETITIVE SEQUENCES, NUCLEIC ACID;

EID: 0020378191     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/0378-1119(82)90199-8     Document Type: Article

References (9)

1. Structural analysis of Tn5
2. Plasmid detection and sizing in single colony lysates
3. A rapid alkaline extraction procedure for screening recombinant plasmid DNA
4. Instability of palindromic DNA in E. coli
5. Precise and nearly precise excision of the fused inverted repeats of Tn 5; common features of recA-independent deletion events in E. coli
6. A restriction endonuclease cleavage map of Tn 5 and location of a region encoding neomycin resistance
7. In vivo consequences of plasmid topology
8. Detection of specific sequences among DNA fragments separated by gel electrophoresis
9. Deletion mutants of plasmid ColE1