Isolation of intracellular membranes by means of sodium carbonate treatment: Application to Endoplasmic Reticulum

Journal of Cell Biology

Volumn 93, Issue 1, 1982, Pages 97-102

  Fujiki, Yukio ^a,b   Hubbard, L ^a,b   Fowler, Stanley ^a,b   Lazarow, Paul B ^a,b  

^a ROCKEFELLER UNIVERSITY   (United States)

^b JOHNS HOPKINS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

SODIUM CARBONATE;

CELL MEMBRANE; ELECTRON MICROSCOPY; ENDOPLASMIC RETICULUM; METHODOLOGY;

ANIMAL; CARBONATES; CELL FRACTIONATION; CENTRIFUGATION, DENSITY GRADIENT; FEMALE; INTRACELLULAR MEMBRANES; LIVER; MICROSCOPY, ELECTRON; MICROSOMES, LIVER; RATS; RATS, INBRED STRAINS; SARCOPLASMIC RETICULUM; SUPPORT, NON-U.S. GOV'T; SUPPORT, U.S. GOV'T, NON-P.H.S.; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0020039866     PISSN: 00219525     EISSN: 15408140     Source Type: Journal    
DOI: 10.1083/jcb.93.1.97     Document Type: Article

References (31)

1. Fleischer, S., and L. Packer, editors. 1974. Methods in Enzymology. Biomembranes, Part A. Academic Press, Inc., New York. 31: 1-709.
2. Steck, T. L. 1974. The organization of proteins in the human red blood cell membrane. J. Cell Biol. 62: 1-19.
3. Beaufay, H., A. Amar-Costesec, D. Thines-Sempoux, M. Wibo, M. Robbi, and J. Berthet. 1974. Analytical study of microsomes and isolated subcellular membranes from rat liver. III. Subfractionation of the microsomal fraction by isopycnic and differential centrifugation in density gradients. J. Cell Biol. 61: 213-231.
4. Amar-Costesec, A., M. Wibo, D. Thines-Sempoux, H. Beaufay, and J. Berthet. 1974. Analytical study of microsomes and isolated subcellular membranes from rat liver. IV. Biochemical, physical, and morphological modifications of microsomal components induced by digitonin, EDTA, and pyrophosphate. J. Cell Biol. 62: 717-745.
5. Adelman, M. R., D. D. Sabatini, and G. Blobel. 1973. Ribosome-membrane interaction. Nondestructive disassembly of rat liver rough microsomes into ribosomal and membrane components. J. Cell Biol. 56: 206-229.
6. Kreibich, G., P. Debey, and D. D. Sabatini. 1973. Selective release of content from microsomal vesicles without membrane disassembly. I. Permeability changes induced by low detergent concentrations. J. Cell Biol. 58: 436-462.
7. Kreibich, G., and D. D. Sabatini. 1974. Selective release of content from microsomal vesicles without membrane disassembly. II. Electrophoretic and immunological characterization of microsomal subfractions. J. Cell Biol. 61: 789-807.
8. Fujiki, Y., S. Fowler, H. Shio, A. L. Hubbard, and P. B. Lazarow. 1982. Polypeptide and phospholipid composition of the membrane of rat liver peroxisomes: comparison with endoplasmic reticulum and mitochondrial membranes. J. Cell Biol. 93: 103-110.
9. Fujiki, Y., S. Fowler, A. L. Hubbard, and P. B. Lazarow. 1981. Comparison of rat liver peroxisome membranes with endoplasmic reticulum and mitochondrial membranes. Fed. Proc. 40: 1616(Abstr.).
10. de Duve, C., B. C. Pressman, R. Gianetto, R. Wattiaux, and F. Appelmans. 1955. Tissue fractionation studies. 6. Intracellular distribution patterns of enzymes in rat liver tissue. Biochem. J. 60: 604-617.
11. Leighton, F., B. Poole, H. Beaufay, P. Baudhuin, J. W. Coffey, S. Fowler, and C. De Duve. 1968. The large-scale separation of peroxisomes, mitochondria, and lysosomes from the livers of rats injected with Triton WR-1339. J. Cell Biol. 37: 482-512.
12. Taylor, J. M., and R. T. Schimke. 1973. Synthesis of rat liver albumin in a rabbit reticulocyte cell-free protein-synthesizing system. J. Biol. Chem. 248: 7661-7668.
13. Lowry, H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193: 265-275.
14. Beaufay, H., A. Amar-Costesec, E, Feytmans, D. Thines-Sempoux, M. Wibo, M. Robbi, and J. Berthet. 1974. Analytical study of microsomes and isolated subcellular membranes from rat liver. I. Biochemical methods. J. Cell Biol. 61: 188-200.
15. Blobel, G., and V. R. Potter. 1968. Distribution of radioactivity between the acid-soluble pool and the pools of RNA in the nuclear, nonsedimentable and ribosome fractions of rat liver after a single injection of labeled orotic acid. Biochim. Biophys. Acta. 166: 48-57.
16. Rogers, M. J., and P. Strittmatter. 1973. Lipid-protein interactions in the reconstitution of the microsomal reduced nicotinamide adenine dinucleotide-cytochrome br, reductase system. J. Biol. Chem. 248: 800-806.
17. Bligh, E. G., and W. J. Dyer. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37: 911-917.
18. Ames, B. N., and D. T. Dubin. 1960. The role of polyamines in the neutralization of bacteriophage deoxyribonucleic acid. J. Biol. Chem. 235: 769-775.
19. Robbi, M., and P. B. Lazarow. 1978. Synthesis of catalase in two cell-free protein-synthesizing systems and in rat liver. Proc. Natl. Acad. Sci. U. S. A. 75: 4344-4348.
20. Farquhar, M. G., and G. E. Palade. 1965. Cell junctions in amphibian skin. J. Cell Biol. 26: 263-291.
21. Amar-Costesec, A. H. Beaufay, M. Wibo, D. Thines-Sempoux, E. Feytmans, M. Robbi. and J. Berthet. 1974. Analytical study of microsomes and isolated subcellular membranes from rat liver. II. Preparation and composition of the microsomal fraction. J. Cell Biol. 61: 201-212.
22. Remade, J., S. Fowler, H. Beaufay, A, Amar-Costesec, and J. Berthet. 1976. Analytical study of microsomes and isolated subcellular membranes from rat liver. VI. Electron microscope examination of microsomes for cytochrome br, by means of a ferritin-labeled antibody. J. Cell Biol. 71: 291-313.
23. Kreibich, G., B. L. Ulrich, and D. D. Sabatini. 1978. Proteins of rough microsomal membranes related to ribosome binding. I. Identification of ribophorins I and II. membrane-proteins characteristic of rough microsomes. J. Cell Biol. 77: 464-487.
24. Lazarow, P. B., and C. De Duve. 1973. The synthesis and turnover of rat liver peroxisomes. V. Intracellular pathway of catalase synthesis. J. Cell Biol. 59: 507-524.
25. Steck, T. L., and J. Yu. 1973. Selective solubilization of proteins from red blood cell membranes by protein perturbants. J. Supramol. Struct. 1: 220-232.
26. Reynolds, J. A., and H. Trayer. 1971. Solubility of membrane proteins in aqueous media. J. Biol. Chem. 246: 7337-7342.
27. Scheele, G. A., G. E. Palade, and A. M. Tartakoff. 1978. Cell fractionation studies on the guinea pig pancreas. Redistribution of exocrine proteins during tissue homogenization. J. Cell Biol. 78: 110-130.
28. Singer, S. J., and G. L. Nicolson. 1972. The fluid mosaic model of the structure of cell membranes. Cell membranes are viewed as two-dimensional solutions of oriented globular proteins and lipids. Science (Wash. D. C.). 175: 720-731.
29. Beaufay, H., P. Jacques, P. Baudhuin, O. Z. Sellinger, J. Berthet, and C. De Duve. 1964. Tissue fractionation studies. 18. Resolution of mitochondrial fractions from rat liver into three distinct populations of cytoplasmic particles by means of density equilibration in various gradients. Biochem. J. 92: 184-205.
30. Kreibich, G., M. Czako-Graham, R. Grebenau, W. Mok, E. Rodriquez-Boulan, and D. D. Sabatini. 1978. Characterization of the ribosomal binding site in rat liver rough microsomes: ribophorins I and II, two integral membrane proteins related to ribosome binding. J. Supramol. Struct. 8: 279-302.
31. Rachubinski, R. A., D. P. S. Verma, and J. J. M. Bergeron. 1980. Synthesis of rat liver microsomal cytochrome br, by free ribosomes. J. Cell Biol. 84: 705-716.