Flow cytometric estimation of DNA and RNA content in intact cells stained with hoechst 33342 and pyronin Y

Cytometry

Volumn 2, Issue 3, 1981, Pages 143-150

  Shapiro, Howard M ^a  

^a DANA FARBER CANCER INSTITUTE   (United States)

Author keywords

DNA content; Flow cytometry; Hoechst 33342; pyronin; RNA content

Indexed keywords

DNA; HOE 33342; PYRONINE; RNA;

CYTOLOGY; FLOW CYTOMETRY; METHODOLOGY;

ACRIDINE ORANGE; ANIMAL; BENZIMIDAZOLES; CELL LINE; COMPARATIVE STUDY; DNA; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE; LYMPHOCYTE ACTIVATION; LYMPHOCYTES; OXAZINES; PYRONINE; RATS; RIBONUCLEASES; RNA; STAINING AND LABELING; SUPPORT, U.S. GOV'T, NON-P.H.S.; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0019805191     PISSN: 01964763     EISSN: 10970320     Source Type: Journal    
DOI: 10.1002/cyto.990020302     Document Type: Article

References (27)

1. Analysis and sorting of living cells according to deoxyribonucleic acid content
2. La détection histochimique des acides pentosenucleiques
3. New cell cycle compartments identified by multiparameter flow cytometry
4. Selection of viable cells with known DNA content
5. Pyronin Y in the methyl green‐pyronin histological stain
6. Simultaneous quantitation of Hoechst 33342 and immunofluorescence on viable cells using a fluorescence activated cell sorter
7. Vital DNA staining and sorting by flow microfluorometry
8. Interactions of heteroaromatic compounds with nucleic acids. 1. The influence of heteroatoms and polarizability on the base specificity of intercalating ligands
9. Interactions of heteroaromatic compounds with nucleic acids. 2. Influence of substituents on the base and sequence specificity of intercalating ligands
10. Interactions of heteroaromatic compounds with nucleic acids. A‐T ‐specific non‐intercalating DNA ligands
11. Mass action and acridine orange staining: Static and flow cytofluorometry
12. 2 + M phases after irradiation of a solid tumor in vivo
13. Methyl‐green‐pyronin as a differential nucleic acid stain for peripheral blood smears
14. Flow cytometric analysis of RNA content per cell using pyronin‐Y and methyl green
15. Identification of monocytes in suspensions of mononuclear cells
16. Histochernical identification of acid mucopolysac‐charides with acridine orange
17. Metachromasia
18. The specificity of the methyl green‐pyronin stain for nucleic acids
19. How cytometry of human reticulocytes based on RNA fluorescence
20. Reduction of l‐β‐D‐arabinofuranosylcytosine and adriamycin cytotoxicity following cell cycle arrest by anguidine
21. Simultaneous staining of ribonucleic and deoxyribonucleic acids in unfixed cells using acridine orange in a flow cytofluorimetric system