The fate of axonal debris in wallerian degeneration of rat optic and sciatic nerves: Electron microscopy and immunofluorescence studies with neurofilament antisera

Journal of Neuropathology and Experimental Neurology

Volumn 40, Issue 5, 1981, Pages 537-550

  Bignami, Amico ^a   Dahl, Doris ^a   Nguyen, Bich T ^a   Crosby, Carol J ^a  

^a VA BOSTON HEALTHCARE SYSTEM   (United States)

Author keywords

Electron; Fluorescent antibody technique; Microscopy; Neurofibrils; Wallerian degeneration

Indexed keywords

ANTISERUM;

ANIMAL EXPERIMENT; ELECTRON MICROSCOPY; IMMUNOFLUORESCENCE; NERVE DEGENERATION; NERVE FIBER; NERVOUS SYSTEM; NEUROFILAMENT; OPTIC NERVE; PERIPHERAL NERVOUS SYSTEM; RAT; SCIATIC NERVE; VISUAL SYSTEM;

ANIMAL; AXONS; CYTOSKELETON; ELECTROPHORESIS, POLYACRYLAMIDE GEL; FLUORESCENT ANTIBODY TECHNIQUE; IMMUNE SERA; MICROSCOPY, ELECTRON; NERVE DEGENERATION; NERVE TISSUE PROTEINS; OPTIC NERVE; RATS; RATS, INBRED F344; SCIATIC NERVE; SUPPORT, U.S. GOV'T, P.H.S.; WALLERIAN DEGENERATION;

EID: 0019404010     PISSN: 00223069     EISSN: 15546578     Source Type: Journal    
DOI: 10.1097/00005072-198109000-00005     Document Type: Article

References (28)

1. Urich H. Diseases of peripheral nerves. In: Blackwood W, Corsellis JAN, McMenemey WH, eds. Greenfield’s neuropathology. 3rd ed. London: Arnold, 1976:688-770
2. Daniel PM, Strich SJ. Histological observations on Wallerian degeneration in the spinal cord of the baboon, Papio papio. Acta Neuropathol (Berl) 1969;12:314-28
3. Lampert PW, Cressman MR. Fine structural changes in myelin sheaths after axonal degeneration in the spinal cord of rats. Am J Pathol 1966;49:1139-55
4. Bignami A, Ralston HJ. The cellular reaction to Wallerian degeneration in the central nervous system of the cat. Brain Res 1969;13:444-61
5. Bignami A, Eng LF. Biochemical studies of myelin in Wallerian degeneration of rat optic nerve. J Neurochem 1973;20:165-73
6. Hoffman PN, Lasek RJ. The slow component of axonal transport. Identification of major structural polypeptides of the axon and their generality among mammalian neurons. J Cell Biol 1975;66:351-66
7. Schlaepfer WW. Immunological and ultrastructural studies of neurofilaments isolated from rat peripheral nerve. J Cell Biol 1977;74:226-40
8. Lazarides E. Intermediate filaments as mechanical integrators of cellular space. Nature 1980;283:249-56
9. Dahl D. Astroglial and axonal proteins in isolated brain filaments. II. Isolation of a 70,000 dalton polypeptide from brain filament preparations of bovine brain by immunoaffinity chromatography with antineurofilament antisera. Biochim Biophys Acta 1980;622:9-17
10. Dahl D, Bignami A. Preparation of antisera to neurofilament protein from chicken brain and human sciatic nerve. J Comp Neurol 1977;176:645-58
11. Dahl D. Isolation of neurofilament proteins and of immunologically active neurofilament degradation products from extracts of brain, spinal cord and sciatic nerve. Biochim Biophys Acta 1981;668:299-306
12. Dahl D, Crosby CJ, Bignami A. Filament proteins in rat optic nerves undergoing Wallerian degeneration. Exp Neurol 1981;71:421-30
13. Bignami A, Dahl D. Astrocyte-specific protein and neuroglial differentiation. An immunofluorescence study with antibodies to the glial fibrillary acidic protein. J Comp Neurol 1974;153:27-38
14. Dahl D, Bignami A. Astroglial and axonal proteins in isolated brain filaments. I. Isolation of the glial fibrillary acidic protein and of an immunologically active cyanogen bromide peptide from brain filament preparations of bovine white matter. Biochim Biophys Acta 1979;578:305-16
15. Rueger DC, Dahl D, Bignami A. Comparison of bovine glial fibrillary acidic protein with bovine brain tubulin. Brain Res 1978;153:188-93
16. Weber K, Osborn M. The reliability of molecular weight determinations by dodecyl sulfate- polyacrylamide gel electrophoresis. J Biol Chem 1969;244:4406-12
17. Dahl D, Bignami A. Protein differences associated with the loss of myelinated axons and fibrillary gliosis in rat optic nerves following Wallerian degeneration. FEBS Lett 1975;51:313-6
18. Bissell MG, Rubinstein LJ, Bignami A, Herman MM. Characteristics of the rat C-6 glioma maintained in organ culture systems. Production of glial fibrillary acidic protein in the absence of gliofibrillogenesis. Brain Res 1974;82:77-89
19. Raju TR, Bignami A, Dahl D. Glial fibrillary acidic protein in monolayer cultures of C-6 glioma cells: Effect of aging and dibutyryl cyclic AMP. Brain Res 1980;200:225-30
20. Tuszynski GS, Frank ED, Damsky CH, Buck CA, Warren L. The detection of smooth muscle desmin-like protein in BHK21/C13 fibroblasts. J Biol Chem 1979;264:6138-43
21. Dahl D, Bignami A, Weber K, Osborn M. Filament proteins in rat optic nerves undergoing Wallerian degeneration. Localization of vimentin, the fibroblastic 100 A filament protein, in normal and reactive astrocytes. Exp Neurol 1981;73 (in press)
22. Schlaepfer WW, Micko S. Chemical and structural changes of neurofilaments in transected rat sciatic nerve. J Cell Biol 1978;78:369-78
23. Schlaepfer WW, Hassler M. The persistence and possible externalization of axonal debris during Wallerian degeneration. J Neuropathol Exp Neurol 1979;38:242-52
24. Cook RD, Ghetti B, Wisniewski HM. The pattern of Wallerian degeneration in the optic nerve of newborn kittens: An ultrastructural study. Brain Res 1974; 75:261-75
25. Cook RD, Wisniewski HM. The role of oligodendroglia and astroglia in Wallerian degeneration of the optic nerve. Brain Res 1973;61:191-206
26. Fulcrand J, Privat A. Neuroglial reactions secondary to Wallerian degeneration in the optic nerve of the postnatal rat: Ultrastructural and quantitative study. J Comp Neurol 1977;176:189-224
27. Vaughn J E, Hinds PL, Skoff RP. Electron microscopic studies of Wallerian degeneration in rat optic nerves. I. The multipotential glia. J Comp Neurol 1970;140:175-206
28. Vaughn JE, Pease DC. Electron microscopic studies of Wallerian degeneration in rat optic nerve. II. Astrocytes, oligodendrocytes and adventitial cells. J Comp Neurol 1970;140:207-26