The entry of diphtheria toxin into the mammalian cell cytoplasm: Evidence for lysosomal involvement

Journal of Cell Biology

Volumn 87, Issue 3, 1980, Pages 849-854

  Draper, Rockford K ^a,b   Simon, Melvin I ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF TEXAS AT DALLAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIPHTHERIA TOXIN; RADIOISOTOPE;

CYTOPLASM; IN VITRO STUDY; LYSOSOME; METHODOLOGY;

AMMONIUM CHLORIDE; ANIMAL; CELL LINE; CELL MEMBRANE; CERCOPITHECUS AETHIOPS; CYTOPLASM; DIPHTHERIA TOXIN; HAMSTERS; HYDROGEN-ION CONCENTRATION; LYSOSOMES; PROTEINS; SUPPORT, U.S. GOV'T, NON-P.H.S.;

EID: 0019287379     PISSN: 00219525     EISSN: 15408140     Source Type: Journal    
DOI: 10.1083/jcb.87.3.849     Document Type: Article

References (21)

1. Boquet, P., M. S. Silverman, A. M. Pappenheimer, Jr., and W. B. Vernon. 1976. Binding of Triton X-100 to diphtheria toxin, crossreacting material 45. and their fragments. Proc. Natl. Acad. Sci. U. S. A. 73: 4449-4453.
2. Collier, R. J. 1975. Diphtheria toxin: mode of action and structure. Bacteriol. Rev. 39: 5485.
3. Collier, R. J., and J. Kandel. 1977. Structure and activity of diphtheria toxin. J. Biol. Chem. 246: 1496-1503.
4. de Duve, C., T. de Barsey, B. Poole, A. Trouet, P. Tulkens, and F. van Hoff. 1974. Lysosomotropic agents. Biochan. Pharmacol 23: 2495-2531.
5. Dorland, R. B., J. L. Middlebrook, and S. H. Leppla. 1979. Receptor-mediated internalization and degradation of diphtheria toxin by monkey kidney cells. J. Biol. Chem. 254: 11337-11342.
6. Draper, R. K., D. Chin, D. Eurey-Owens, I. E. Scheftler, and M. I. Simon. 1979. Biochemical and genetic characterization of three hamster cell mutants resistant to diphtheria toxin. J. Cell Biol. 83: 116-125.
7. Draper, R. K., D. Chin, and M. I. Simon. 1978. Diphtheria toxin has the properties of a lectin. Proc. Natl. Acad. Sci U. S. A. 75: 261-265.
8. Draper, R. K., D. Chin, L. Stubbs, and M. I. Simon. 1978. Studies of the diphtheria toxin receptor on Chinese hamster cells. J, Supramol. Struct. 9: 47-55.
9. Goldstein, J. L., R. G. W. Anderson, and M. S. Brown. 1979. Coated pits, coated vesicles, and receptor-mediated endocytosis. Nature (Lond.). 279: 679-685.
10. Gordon, G. B., L. R. Miller, and K. G. Bensch. 1965. Studies on the intracellular digestive process in mammalian tissue culture cells. J. Cell. Biol. 25(2, Pt. 2): 41-53.
11. Helenius, A., J. Kartenbeck, K. Simons, and E. Fries. 1979. On the entry of Semliki Forest virus into BHK-21 cells. J. Cell Biol. 84: 404-420.
12. Ivins, B., C. B. Saelinger, P. F. Bonventre, and C. Woscinski. 1975. Chemical modulation of diphtheria toxin action on cultured mammalian cells. Infect. Immun. 11: 665-674.
13. Kim, K., and N. B. Groman. 1965. In vitro inhibition of diphtheria toxin action by ammonium salts and amines. J. Bacteriol. 90: 1552-1556.
14. Kim, K., and N. B. Groman. 1965. Mode of inhibition of diphtheria toxin by ammonium chloride. J. Bacteriol. 90: 1557-1562.
15. Leppla, S. H., R. B. Dorland, and J. L. Middlebrook. 1980. Inhibition of diphtheria toxin degradation and cytotoxic action by chloroquine. J. Biol Chem. 255: 2247-2250.
16. Louvard, D., C. Vannier, S. Maroux, J. M. Pages, and C. Lazdunski. 1976. A quantitative immunochemical technique for evaluation of the extent of integration of membrane proteins and of protein conformation changes and homologies. Anal Biochem. 76: 83-94.
17. Middlebrook, J. L., R. B. Dorland, and S. H. Leppla. 1979. Effects of lectins on the interaction of diphtheria toxin with mammalian cells. Exp. Cell Res. 121: 95-101.
18. Middlebrook, J. L., and R. B. Dorland. 1977. Differential chemical protection of mammalian cells from the effects of the exotoxins of Corynebacterium diphtheriae and Pseudomonas aeruginosa. Infect. Immunol 16: 232-239.
19. Nicolson, G. L. 1974. Ultrastructural analysis of toxin binding and entry into mammalian cells. Nature (Lond.). 251: 628-630.
20. Ohkuma, S., and B. Poole. 1978. Fluorescence probe measurement of the intralysosomal pH in living cells and the perturbation of pH by various agents. Proc. Natl. Acad. Sci. U. S.A. 75: 3327-3331.
21. Pappenheimer, A. M., Jr. 1977. Diphtheria toxin. Annu. Rev. Biochem. 46: 69-94.