Immunochemistry on ultrathin frozen sections

The Histochemical Journal

Volumn 12, Issue 4, 1980, Pages 381-403

  Tokuyasu, K T ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FIXATIVE; RIBONUCLEASE;

ANIMAL; ARTICLE; CATTLE; ELECTRON MICROSCOPY; ENZYMOLOGY; FROZEN SECTION; IMMUNOCHEMISTRY; METABOLISM; METHODOLOGY; MICROTOMY; PANCREAS; RAT; ULTRASTRUCTURE;

ANIMAL; CATTLE; FIXATIVES; FROZEN SECTIONS; IMMUNOCHEMISTRY; MICROSCOPY, ELECTRON; MICROTOMY; PANCREAS; RATS; RIBONUCLEASES; SUPPORT, U.S. GOV'T, P.H.S.;

EID: 0019042936     PISSN: 00182214     EISSN: 15736865     Source Type: Journal    
DOI: 10.1007/BF01011956     Document Type: Article

References (60)

1. The possibilities of locating soluble labeled compounds by electron microscope autoradiography
2. A cryostat approach to ultrathin ‘dry’ frozen sections for electron microscopy: a morphological and X-ray analytical study
3. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies
4. Méthode de marquage d'antigènes et d'anticorps avec des enzymes et son application en immunodiffusion
5. Synthesis of water-soluble undecagold cluster compounds of potential importance in electron microscopic and other studies of biological systems
6. Localization of vicilin peptidohydrolase in the cotyledons of mung bean seedlings by immunofluorescence microscopy
7. Ultrathin frozen sections. I. Methods and ultrastructural preservation
8. Coupes a congelation ultrafines de tissus inclus dans la gelatine
9. Improved techniques for the preparation of ultrathin frozen sections
10. Detection of DNA by tritiated actinomycin D on ultrathin frozen sections
11. Localization of an endogenous lectin in chicken liver, intestine and pancreas
12. The capping of lymphocytes and other cells, studied by an improved method of immunofluorescence staining of frozen sections
13. A way to prepare frozen thin sections of fresh tissue for electron microscopy
14. Frozen thin sections of fresh tissue for electron microscopy, with a description of pancreas and liver
15. Immunoelectron microscopy and immunofluorescence in membrane biology
16. Iron-dextran antibody conjugates; a general method for the simultaneous staining of two components in high resolution immunoelectron microscopy
17. The submicroscopic organization of vertebrate nerve fibres. An electron microscopic study of myelinated and unmyelinated nerve fibres
18. The immunocytochemical localization of α-actinin in intestinal epithelial cells
19. Immunocytochemical localization of amylase and chymotrypsinogen in the exocrine pancreatic cell with special attention to the Golgi complex
20. An anti-roll plate for flattening of ‘dry’ ultrathin frozen sections
21. Colloidal gold, a useful marker for transmission and scanning electron microscopy
22. Ultracryotomy: A routine procedure
23. Localization of tissue antigens of the ultrathin sections with peroxidase-labeled antibody method
24. Two improved methods for preparing ferritin-protein conjugates for electron microscopy
25. Mounting of ultrathin sections with the aid of an electrostatic field
26. Solid phase conjugation of ferritin to Fab fragments for use in antigen localization on thin sections
27. Immunocytochemical localization of secretory proteins in bovine pancreatic exocrine cells
28. Immunoferritin determination of the distribution of (Na+−K+) ATPase over the plasma membranes of renal convoluted tubules. I. Distal segment
29. Immunoferritin determination of the distribution of (Na+−K+) ATPase over the plasma membranes of renal convoluted tubules. II. Proximal segment
30. Ultrathin frozen sections. II. Demonstration of enzymatic activity
31. Alteration of the conformation of proteins in red cell membranes and in solution by fixatives used in electron microscopy
32. A general method for the specific staining of intracellular antigens with ferritin-antibody conjugates
33. Classification of anterior pituitary cell types with immunoenzyme histochemistry
34. Enzyme-labeled antibodies: preparation and application for the localization of antigens
35. Enzyme-labeled antibodies for the light and electron microscopic localization of tissue antigen
36. Further characterization of embryonic tendon fibroblasts and the use of immunoferritin techniques to study collagen biosynthesis
37. Individual microtubules viewed by immunofluorescence and electron microscopy in the same PtK2 cell
38. Immunoferritin localization of intracellular antigens: the use of ultracryotomy to obtain ultrathin sections suitable for direct immunoferritin staining
39. Immunocytochemical localization of opsin in outer segments and Golgi zones of frog photoreceptor cells
40. Immunocytochemical localization of a large intrinsic membrane protein to the incisures and margins of frog rod outer segment disks
41. Disordeting of myofibril structure due to fixation, dehydration and embedding
42. Cytochemistry and electron microscopy: The preservation of cellular ultrastructure and enzymatic activity by aldehyde fixation
43. Preparation of an electron-dense antibody conjugate
44. Immunoelectron microscopy with ferritin conjugates
45. The properties of specific stains for electron microscopy prepared by the conjugation of antibody molecules with ferritin
46. The structure of mitochondrial membranes in frozen sections
47. Fine structure of the A-band in cryosections. The structure of the A-band of human skeletal muscle fibres from ultra-thin cryo-section negatively stained
48. Enzyme immunocytochemistry
49. A technique for ultracryotomy of cell suspensions and tissues
50. Some applications of cryo-ultramicrotomy
51. Membranes as observed in frozen sections
52. A study of positive staining of ultrathin frozen sections
53. Immunoferritin localization of intracellular antigens in positively stained frozen sections
54. Domains of receptor mobility and endocytosis in the membranes of neonatal human erythrocytes and reticulocytes are deficient in spectrin
55. Improved procedures for immunoferritin labeling of ultrathin frozen sections
56. Simultaneous observations of immunolabeled frozen sections in LM and EM
57. Ultrastructural antibody localization of α2 macroglobulin in membrane-limited vesicles in cultured cells