Assessment of Growth-Limiting Events Caused by 5-Fluorouracil in Mouse Cells and in Human Cells

Cancer Research

Volumn 40, Issue 11, 1980, Pages 4113-4122

  Hakala, Maire T ^c  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

^b College of Physicians and Surgeons ^*  (United States)

^c ROSWELL PARK CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FLUOROURACIL; RADIOISOTOPE; THYMIDYLATE SYNTHASE;

ANIMAL EXPERIMENT; CARCINOMA; CELL CULTURE; CELL GROWTH; DEOXYURIDINE C 14; FIBROBLAST; FLOXURIDINE C 14; FLUOROURACIL C 14; HELA CELL; HUMAN CELL; IN VITRO STUDY; MOUSE; SARCOMA; THYMIDINE C 14;

ANIMAL; CELL DIVISION; CELLS, CULTURED; DEOXYRIBONUCLEOTIDES; DEOXYURIDINE; DNA; DOSE-RESPONSE RELATIONSHIP, DRUG; FLUOROURACIL; HUMAN; METHYLTRANSFERASES; MICE; RNA; SARCOMA, EXPERIMENTAL; SUPPORT, U.S. GOV'T, P.H.S.; THYMIDINE MONOPHOSPHATE; THYMIDYLATE SYNTHASE;

EID: 0018926163     PISSN: 00085472     EISSN: 15387445     Source Type: Journal    
DOI: None     Document Type: Article

References (43)

1. Andoh, T., and Chargaff, E. Formation and fate of abnormal ribosomes of E. coli cells treated with 5-fluorouracil. Proc. Natl. Acad. Sci. U. S. A., 54: 1181–1189, 1965.
2. Ardalan, B., Buscaglia, M. D., and Schein, P. S. Tumor 5-fluorodeoxyuridy-late concentration as a determinant of 5-fluorouracil response. Biochem. Pharmacol., 27: 2009–2013, 1978.
3. Bosch, L., Harbers, E., and Heidelberger, C. Studies on fluorinated pyrimidines V. Effects on nucleic acid metabolism in vitro. Cancer Res., 18: 335–343, 1958.
4. Carrico, C. K., and Glazer, R. I. Effect of 5-fluorouracil on the synthesis and translation of polyadenylic acid-containing RNA from regenerating rat liver. Cancer Res., 39: 3694–3701.1979.
5. Chaudhuri, N. K., Montag, B. J., and Heidelberger, C. Studies on fluorinated pyrimidines III. The metabolism of 5-fluorouracil-2-C14 and 5-fluoroorotic-2-C14 acid in vivo. Cancer Res., 18: 318–328, 1958.
6. Danenberg, P. V. Thymidylate synthetase—a target enzyme in cancer chemotherapy. Biochim. Biophys. Acta. 473: 73–92, 1977.
7. Danenberg, P. V., and Danenberg, K. D. Effect of 5,10-methylenetetrahydrofolate on the dissociation of 5-fluoro-2́-deoxyuridylate from thymidylate synthetase: evidence for an ordered mechanism. Biochemistry, 17: 4018–4024, 1978.
8. Divekar, A. Y., and Hakala, M. T. Adenosine kinase of Sarcoma 180 cells. N6-substituted adenosines as substrates and inhibitors. Mol. Pharmacol., 7: 663–673, 1971.
9. Dolnick, B. J., and Cheng, Y-C. Human thymidylate synthetase derived from blast cells of patients with acute myelocytic leukemia. J. Biol. Chem., 252: 7697–7703, 1977.
10. Eagle, H. Amino acid metabolism in mammalian cell cultures. Science (Wash. D. C.). 130: 432–437, 1959.
11. Evans, R. M., and Hakala, M. T. Critical sites of action of 5-fluorouracil (FU) in human and mouse cells in culture. Fed. Proc., 37: 2335, 1978.
12. Fridland, A., Langenbach, R. J., and Heidelberger, C. Purification of thymidylate synthetase from Ehrlich ascites carcinoma cells. J. Biol. Chem., 246:7110–7114, 1972.
13. Hakala, M. T. Prevention of toxicity of amethopterin for Sarcoma 180 cells in tissue culture. Science (Wash. D. C.), 126: 255, 1957.
14. Hakala, M. T. Transport of antineoplastic agents. In: A. C. Sartorelli and D. G. Johns (eds.). Handbook of Experimental Pharmacology. Vol. 38. pp. 240–269. Berlin: Springer-Verlag, 1974.
15. Hakala, M. T., Holland, J. F., and Horoszewicz, J. S. Change in pyrimidine deoxyribonucleoside metabolism in cell culture caused by Mycoplasma (PPLO) contamination. Biochem. Biophys. Res. Commun., 11: 466–471, 1961.
16. Hakala, M. T., and Rustum, Y. M. The potential value of in vitro screening. In: V. T. DeVita, Jr., and H. Busch (eds.), Methods in Cancer Research, Cancer Drug Development, Part A, pp. 247–287. New York: Academic Press, Inc., 1979.
17. Hakala, M. T., and Taylor, E. The ability of purine and thymidine derivatives and of glycine to support the growth of mammalian cells in culture. J. Biol. Chem., 234: 126–128, 1959.
18. Harbers, E., Chaudhuri, N. K., and Heidelberger, C. Studies on fluorinated pyrimidines VII. Further biochemical and metabolic investigations. J. Biol. Chem., 234: 1255–1262, 1959.
19. Heidelberger, C. Fluorinated pyrimidines and their nucleosides. In: Handbook of Experimental Pharmacology, Vol. 38, Part 2, pp. 193–231. Berlin: Springer-Verlag, 1974.
20. Hills, D. C., and Horowitz, J. Ribosome synthesis in Escherichia coli treated with 5-fluorouracil. Biochemistry, 5: 1625–1632, 1966.
21. Klubes, P., Connelly, K., Cerna, I., and Mandel, H. G. Effects of 5-fluorouracil on 5-fluorodeoxyuridine-5́-monophosphate and 2-deoxy-uridine-5́-monophosphate pools, and DNA synthesis in solid mouse L1210 and rat Walker 256 tumors. Cancer Res., 38: 2325–2331. 1978.
22. Langenbach, R. J., Danenberg, P. V., and Heidelberger, C. Thymidylate synthetase: mechanism of inhibition by 5-fluoro-2́-deoxyuridylate. Biochem. Biophys. Res. Commun., 48: 1565–1571, 1972.
23. Laskin, J. D., Evans, R. M., Slocum, H. K., Burke, D., and Hakala, M. T. Basis for natural variation in sensitivity to 5-fluorouracil in mouse and human cells in culture. Cancer Res., 39: 383–390, 1979.
24. Laskin. J. D., and Hakala, M. T. Metabolism of 5-fluorouracil (FU) in mouse and human cells as a basis for their differing sensitivity. Proc. Am. Assoc. Cancer Res., 18: 57, 1977.
25. Lowry, O. H., Rosebrough, N. J., Farr, A. L., and Randall, R. J. Protein measurement with the Folin phenol reagent. J. Biol. Chem., 193: 265–275, 1951.
26. Madoc-Jones, H., and Bruce, W. On the mechanism of the lethal action of 5-fluorouracil on mouse L-cells. Cancer Res., 28: 1976–1981. 1968.
27. Mandel, H. G. The incorporation of 5-fluorouracil into RNA and its molecular consequences. Prog. Mol. Subcell. Biol., 1: 82–135, 1969.
28. Martin, D. S., Stolfi, R. L., and Spiegelman, S. Striking augmentation of the in vivo anticancer activity of 5-fluorouracil (FU) by combination with pyrimidine nucleosides. Proc. Am. Assoc. Cancer Res., 19: 221, 1978.
29. Moran, R. G., Spears, C. P., and Heidelberger, C. Biochemical determinants of tumor sensitivity to 5-fluorouracil: ultrasensitive methods for the determination of 5-fluoro-2́-deoxyuridylate, 2́-deoxyuridylate, and thymidylate synthetase. Proc. Natl. Acad. Sci. U. S. A., 76: 1456–1460, 1979.
30. Myers, E. M., Young. R. C., and Chabner, B. A. Biochemical determinants of 5-fluorouracil response in vivo. The role of deoxyuridylate pool expansion. J. Clin. Invest., 56: 1231–1238, 1975.
31. Reeves, W. J., Jr., and Cailleau, R. Mechanism of growth inhibition by 5-fluorouracil. Reversal studies with pyrimidine metabolites in vivo. Proc. Soc. Exp. Biol. Med., 131: 1068–1072, 1969.
32. Rich, M. A., Bolaffi, J. L., Knoll, J. E., Cheong, L., and Eidinoff, M. L. Growth inhibition of a human tumor cell strain by 5-fluorouracil, 5-fluorouridine, and 5-fluoro-2́-deoxyuridine—reversal studies. Cancer Res., 18: 730–735, 1958.
33. Rich, M. A., Saslaw, L., and Eidinoff, M. L. Some differences among various mammalian cell lines in reversal of growth inhibition by 5-fluorouracil. Proc. Soc. Exp. Biol. Med., 103: 791–796, 1960.
34. Roberts, D. An isotopic assay for thymidylate synthetase. Biochemistry. 5: 3546–3548, 1966.
35. Schneider, W. C. Determination of nucleic acids in tissues by pentose analysis. Methods Enzymol., 3: 680–684, 1957.
36. Sköld O. Nucleoside and nucleotide derivatives of 5-fluorouracil. Formation with enzymes from Ehrlich ascites tumor. Ark. Kemi, 17: 59–71, 1960.
37. Slocum, H. K., and Hakala, M. T. Mechanism of natural resistance to N6-(Δ2-isopentenyl) adenosine in cultured cells. Cancer Res., 35: 423–428, 1975.
38. Tseng, W. C., Medina, D., and Randerath, K. Specific inhibition of transfer RNA methylation and modification in tissues of mice treated with 5-fluorouracil. Cancer Res., 38: 1250–1257, 1978.
39. Umeda, M., and Heidelberger, C. Comparative studies of fluorinated pyrimidines with various cell lines. Cancer Res., 28: 2529–2538, 1968.
40. Washtien, W. L., and Santi, D. V. Assay of intracellular free and macromolecular-bound metabolites of 5-fluorodeoxyuridine and 5-fluorouracil. Cancer Res., 39: 3397–3404, 1979.
41. Weiss, J. W., and Pitot, H. C. Inhibition of ribosomal RNA maturation in Novikoff hepatoma cells by toyocamycin, tubercidin, and 6-thioguanine. Cancer Res., 34: 581–587, 1974.
42. Wilkinson, D. S., and Crumley, J. Metabolism of 5-fluorouracil in sensitive and resistant Novikoff hepatoma cells. J. Biol. Chem., 252: 1051–1056, 1977.
43. Zardi, L., and Baserga, R. Ribosomal RNA synthesis in WI-38 cells stimulated to proliferate. Exp. Mol. Pathol., 20: 69–77, 1974.