Testosterone secretion by rat, rabbit, guinea pig, dog, and hamster testes perfused in vitro: Correlation with leydig cell mass

Endocrinology

Volumn 105, Issue 5, 1979, Pages 1135-1142

  Ewing, L L ^a   Zirkin, B R ^a   Cochran, R C ^a   Kromann, N ^a   Peters, C ^a,b   Ruiz Bravo, N ^a,c  

^a JOHNS HOPKINS BLOOMBERG SCHOOL OF PUBLIC HEALTH   (United States)

^b JOHNS HOPKINS UNIVERSITY   (United States)

^c YALE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

LUTEINIZING HORMONE;

ANIMAL EXPERIMENT; ENDOCRINE SYSTEM; IN VITRO STUDY; LEYDIG CELL; MALE GENITAL SYSTEM;

ANIMALS; CRICETINAE; DOGS; GUINEA PIGS; HISTOCYTOCHEMISTRY; LEYDIG CELLS; LUTEINIZING HORMONE; MALE; ORGAN SIZE; PERFUSION; RABBITS; RATS; SPECIES SPECIFICITY; TESTIS; TESTOSTERONE;

EID: 0018604048     PISSN: 00137227     EISSN: 19457170     Source Type: Journal    
DOI: 10.1210/endo-105-5-1135     Document Type: Article

References (36)

1. Chubb, C., and L. L. Ewing, Steroid secretion by in vitro perfused testes: secretions of rabbit and rat testes, Am J Physiol, in press.
2. Bouin, P., and P. Ancel, Recherces sur les cellules interstitielles du testicule des mammiferes, Arch Zool Exp Gen Serv IV 1: 437, 1903.
3. l4C in vitro, Endocrinology 76: 646, 1965.
4. Hall, P. F., D. C. Irby, and D. M. deKretser, Conversion of cholesterol to androgens by rat testes: comparison of interstitial cells and seminiferous tubules, Endocrinology 84: 488, 1969.
5. Fawcett, D. W., W. B. Neaves, and M. N. Flores, Comparative observations on intertubular lymphatics and the organization of the interstitial tissue of the mammalian testis, Endocrinology 9: 500, 1973.
6. Fawcett, D. W., Observations on the organization of the interstitial tissue of the testis and on the occluding cell junctions in the seminiferous junction, Adv Biosci 10: 83, 1973.
7. Kothari, L. K., M. K. Patri, and M. L. Jain, The total Leydig cell volume of the testis in some common mammals, Andrologia 10: 218, 1978.
8. Kothari, L. K., D. K. Srivastava, P. Mishra, and M. K. Patri, Total Leydig cell volume and its estimation in dogs and in models of testis, Anat Rec 174: 259, 1972.
9. Harbitz, T. B., Morphometric studies of the Leydig cells in elderly men with special reference to the histology of the prostate, Acta Pathol Microbiol Scand [A] 81: 301, 1973.
10. Kothari, L. K., and A. S. Gupta, Effect of ageing on the volume, structure and total Leydig cell content of the human testis, Int J Fertil 19: 140, 1974.
11. Pirke, K. M., H.-J. Vogt, and M. Geiss, In vitro and in vivo studies on Leydig cell function in old rats, Acta Endocrinol (Kbh) 89: 393, 1978.
12. Kaler, L. W., and W. B. Neaves, Attrition of the human Leydig cell population with advancing age, Anat Rec 192: 513, 1978.
13. Tamaoki, B., H. Inano, and H. Nakano, In vitro synthesis and conversion of androgens in testicular tissue, In McKerns, K. W. (ed.), The Gonads, Appleton-Century-Crofts, New York, 1969, p. 547.
14. Christensen, A. K., The fine structure of testicular interstitial cells in guinea pigs, J Cell Biol 26: 911, 1965.
15. Christensen, A. K., and S. W. Gillim, The correlation of fine structure and function in steroid-secreting cells with emphasis on those of the gonads, In McKerns, K. W. (ed.), The Gonads, Apple-ton-Century-Crofts, New York, 1969, p. 415.
16. Wing, T.-Y., and H-S. Lin, The fine structure of testicular interstitial cells in the adult golden hamster with special reference to seasonal changes, Cell Tissue Res 183: 385, 1977.
17. Ito, S., and M. J. Karnovsky, Formaldehyde-glutaraldehyde fixatives containing trinitro compounds, J Cell Biol 39: 168, 1968 (Abstract).
18. Weibel, E. R., and R. P. Bolender, Stereological techniques for electron microscopic morphometry, In Hayat, M. A. (ed.), Principles and Techniques of Electron Microscopy, Van-Nostrand-Rein-hold, New York, 1973, p. 239.
19. VanDemark, N. L., and L. L. Ewing, Factors affecting testicular metabolism and function. I. A simplified perfusion technique for short-term maintenance of rabbit testis, J Reprod Fertil 6: 1, 1963.
20. Ewing, L. L., B. Brown, D. C. Irby, and I. Jardine, Testosterone and 5a-reduced androgen secretion by rabbit testes-epididymides perfused in vitro, Endocrinology 96: 610, 1975.
21. Duncan, D. B., Multiple range tests for correlated and heterosce-dastic means, Biometrics 13: 164, 1957.
22. Setchell, B. P., The Mammalian Testis, Paul Elek, London, 1978, p. 122.
23. Wheaton, L. G., D. P. deKlerk, J. D. Strandberg, and D. S. Coffey, Relationship of semen volume to prostate size and pathology in the beagle, Am J Vet Res, in press.
24. Bartlett, D. J., Studies on dog semen. I. Morphological characteristics, J Reprod Fertil 3: 173, 1962.
25. Goodwin, D. E., The Effect of Growth Hormone on Testosterone Secretion in Perfused Rabbit Testes, Ph.D. Thesis, Oklahoma State University, Stillwater, OK, 1972.
26. Chubb, C., L. L. Ewing, D. C. Irby, and C. Desjardins, Testicular maturation in the rabbit: secretion of testosterone, dihydrotestosterone, 5α-androstan-3α, 17β-diol, 5α-androstan-3β, 17β-diol by perfused rabbit testes-epididymides and spermatogenesis, Biol Reprod 18: 212, 1978.
27. Ewing, L. L., B. H. Johnson, C. Desjardins, and R. F. Clegg, Effect of age upon the spermatogenic and steroidogenic elements of rabbit testes, Proc Soc Exp Biol Med 140: 907, 1972.
28. Ewing, L. L., Testis: changes with age, In Grayhack, J. T., J. D. Wilson, and M. J. Scherbenske (eds.), Benign Prostatic Hyperplasia, DHEW Publication (NIH) 76-113, Washington DC, 1975, p. 195.
29. Ewing, L. L., C. Desjardins, D. C. Irby, and B. Robaire, Synergistic interaction of testosterone and oestradiol inhibits spermatogenesis in rats, Nature 269: 409, 1977.
30. Ewing, L. L., B. Schanbacher, C. Desjardins, and V. Chaffee, The effect of subdermal testosterone filled polydimethylsiloxane implants on spermatogenesis in rhesus monkeys (Macaca mulata), Contraception 13: 583, 1976.
31. Waites, G. M. H., Temperature regulation and the testis, In Johnson, A. D., and W. R. Gomes (eds.), The Testis, vol. 1, Academic Press, New York, 1970, p. 241.
32. Setchell, B. P., Testicular blood supply, lymphatic drainage, and secretion of fluid, In Johnson, A. D., and W. R. Gomes (eds.), The Testis, vol. 1, Academic Press, New York, 1970, p. 101.
33. Ewing, L. L., Some factors affecting testosterone, dihydrotestosterone, 5α-androsten-3α, 17β-diol and 5α-androstan-3β, 17β-diol secretion by in vitro perfused rabbit testes, In French, F. S., V. Hansson, E. M. Ritzen, and S. N. Nayfeh (eds.), Hormonal Regulation of Spermatogenesis, Plenum Press, New York, 1975, p. 145.
34. Johnson, B. H., and L. L. Ewing, Follicle-stimulating hormone and the regulation of testosterone secretion in rabbit testes, Science 173: 635, 1971.
35. Mori, H., and A. K. Christensen, Morphometric analysis of Leydig cells in normal rat testis, J Cell Biol, in press.
36. Ewing, L. L., C. E. Chubb, and B. Robaire, Macromolecules, steroid binding and testosterone secretion by rabbit testes, Nature 264: 84, 1976.