Natural pH gradients formed by amino acids: Ampholyte distribution, time course, use in electrofocusing of protein, relation to pH gradients in isotachophoresis, and separator effects

Analytical Biochemistry

Volumn 81, Issue 1, 1977, Pages 28-39

  Caspers, Mary Lou ^a   Chrambach, Andreas ^b  

^a WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b EUNICE KENNEDY SHRIVER NATIONAL INSTITUTE OF CHILD HEALTH AND HUMAN DEVELOPMENT   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; PROTEIN; BOVINE SERUM ALBUMIN; BUFFER;

IN VITRO STUDY; ISOTACHOPHORESIS; METHODOLOGY; THEORETICAL STUDY; ARTICLE; ISOELECTRIC FOCUSING; ISOLATION AND PURIFICATION; PH; TIME;

AMINO ACIDS; BUFFERS; HYDROGEN-ION CONCENTRATION; ISOELECTRIC FOCUSING; PROTEINS; SERUM ALBUMIN, BOVINE; TIME FACTORS;

EID: 0017509483     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/0003-2697(77)90595-4     Document Type: Article

References (26)

1. Natural pH gradients in buffer mixtures: Formation in the absence of strongly acidic and basic anolyte and catholyte, gradient steepening by sucrose, and stabilization by high buffer concentrations in the electrolyte chambers
2. Isoelectric Fractionation, Analysis, and Characterization of Ampholytes in Natural pH Gradients. IX. A Method for Obtaining pH Gradients in the Region Below pH 3, Stable Enough to Permit Isoelectric Focusing of Ampholytes.
3. Isoelectric Fractionation, Analysis, and Characterization of Ampholytes in Natural pH Gradients. II. Buffering Capacity and Conductance of Isoionic Ampholytes.
4. Recovery of protein in preparative polyacrylamide gel electrophoresis