Transfer of proteins across membranes: II. reconstitution of functional rough microsomes from heterologous components

Journal of Cell Biology

Volumn 67, Issue 3, 1975, Pages 852-862

  Blobel, Günter ^a   Dobberstein, Bernhard ^a  

^a ROCKEFELLER UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN G; MESSENGER RNA; PROTEIN;

DOG; IN VITRO STUDY; MEMBRANE; MICROSOME; PANCREAS; RETICULOCYTE; THEORETICAL STUDY;

ANIMAL; CELL FRACTIONATION; CELL MEMBRANE; DOGS; EDETIC ACID; GLOBINS; IMMUNOGLOBULINS, LIGHT-CHAIN; MICROSOMES; PANCREAS; POTASSIUM CHLORIDE; PUROMYCIN; RIBOSOMES; RNA, MESSENGER; SUPPORT, U.S. GOV'T, P.H.S.; TRANSLATION, GENETIC;

EID: 0016752682     PISSN: 00219525     EISSN: 15408140     Source Type: Journal    
DOI: 10.1083/jcb.67.3.852     Document Type: Article

References (7)

1. ADELMAN, M. R., D. D. SABATINI, and G. BLOBEL. 1973. Ribosome-membrane interaction. Nondestructive disassembly of rat liver rough microsomes into ribosomal and membranous components. J. Cell Biol. 56: 206-229.
2. BLOBEL, G., and B. DOBBERSTEIN. 1975. Transfer of proteins across membranes. I. Presence of proteolyti-cally processed and nonprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J. Cell Biol. 67: 835-851.
3. BLOBEL, G., and D. D. SABATINI. 1971. Ribosome-membrane interaction in eukaryotic cells. In Biomembranes. L. A. Manson, editor. Plenum Publishing Corporation, New York. 2: 193-195.
4. BORGESE, N., W. MOK, G. KREIBICH, and D. D. SABATINI. 1974. Ribosomal-membrane membrane interaction: In vitro binding of ribosomes to microsomal membranes. J. Mol. Biol. 88: 559-580.
5. MILSTEIN, C, G. G. BROWNLEE, T. M. HARRISON, and M. B. MATHEWS. 1972. A possible precursor of immunoglobulin light chains. Nat. New Biol. 239: 117-120.
6. SABATINI, D. D., and G. BLOBEL. 1970. Controlled proteolysis of nascent polypeptides in rat liver cell fractions. II. Location of the polypeptides in rough microsomes. J. Cell Biol. 45: 146-157.
7. SABATINI, D. D., Y. TASHIRO, and G. E. PALADE. 1966. On the attachment of ribosomes to microsomal membranes. J. Mol. Biol. 19: 503-524.