Aburatubolactam A, a novel inhibitor of superoxide anion generation from a marine microorganism

Heterocyclic Communications

Volumn 2, Issue 4, 1996, Pages 315-318

  Bae, Myung Ae ^a   Yamada, Kaoru ^a   Ijuin, Yasuharu ^a   Tsuji, Tomoko ^a   Yazawa, Kazunaga ^a   Tomono, Yasuhiko ^b   Uemura, Daisuke ^b  

^a SAGAMI CHEMICAL RESEARCH CENTER   (Japan)

^b SHIZUOKA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0013617464     PISSN: 07930283     EISSN: None     Source Type: Journal    
DOI: 10.1515/HC.1996.2.4.315     Document Type: Article

References (24)

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14. This microorganism was taken from the surface of a mollusk collected on the seashore near Aburatubo, Kanagawa Prefecture in Japan.
15. 6 cells/ mL), and test samples were added. The mixture was preincubated for 15 min. at 37 °C and then incubated for 1 h. after the addition of cytochrome C (final concentration: 2 mg/mL, Sigma Ltd.) containing TPA (final concentration: 100 ng/mL Sigma Ltd.) The optical density of the suspension was measured at 550 nm (ref. 570 nm). The extent of Superoxide anion generation was expressed as absorbance relative to a blank test (cytochrome C, TPA and SOD).
16. 6 cells/ mL), and test samples were added. The mixture was preincubated for 15 min. at 37 °C and then incubated for 1 h. after the addition of cytochrome C (final concentration: 2 mg/mL, Sigma Ltd.) containing TPA (final concentration: 100 ng/mL Sigma Ltd.) The optical density of the suspension was measured at 550 nm (ref. 570 nm). The extent of Superoxide anion generation was expressed as absorbance relative to a blank test (cytochrome C, TPA and SOD).
17. 6 cells/ mL), and test samples were added. The mixture was preincubated for 15 min. at 37 °C and then incubated for 1 h. after the addition of cytochrome C (final concentration: 2 mg/mL, Sigma Ltd.) containing TPA (final concentration: 100 ng/mL Sigma Ltd.) The optical density of the suspension was measured at 550 nm (ref. 570 nm). The extent of Superoxide anion generation was expressed as absorbance relative to a blank test (cytochrome C, TPA and SOD).
18. 5): 13.0 (C-29), 19.6 (C-27), 26.5 (C-28), 29.0, 32.4, 36.0, 38.0, 40.8, 41.8, 45.7, 47.9, 49.8, 50.5, 54.5, 56.7, 71.6 (C-22), 74.0 (C-23), 101.9 (C-20), 121.5, 126.2, 127.0, 132.4, 134.8, 139.4, 143.7, 144.2, 166.5 (C-1). 173.4 (C-19), 174.3 (C-26), 192.7 (C-21).
19. 3, Z = 4, and Cu K-α (λ = 1.54178). The Mac Science MXC 18 instrument was used throughout this work. The final R value was 0.072 for 2302 reflections.
20. ORTEP crystallographic illustration program: Johnson, C. K. Report ORNL-3794, Oak Ridge, TN.
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24. 50 value of this compound was 2.7 μg/mL, and its cytotoxicity against neutrophils was negligible in our experiments. The chemical and biological properties of aburatubolactams will be reported elsewhere.