PCR analysis of pulsed-field gel electrophoresis-purified plastid DNA, a sensitive tool to judge the hetero-/homoplastomic status of plastid transformants

Current Genetics

Volumn 43, Issue 1, 2003, Pages 45-53

  Swiatek, Magdalena ^a   Greiner, Stephan ^a   Kemp, Sabine ^a   Drescher, Anja ^a   Koop, Hans Ulrich ^a   Herrmann, Reinhold G ^a   Maier, Rainer M ^a  

^a UNIVERSITY OF MUNICH   (Germany)

Author keywords

Heteroplastomy; Homoplastomy; Plastid transformation; Pulsed field electrophoresis

Indexed keywords

DNA;

ARTICLE; BIOTECHNOLOGY; CELL NUCLEUS; CHROMOSOME ANALYSIS; CHROMOSOME ISOLATION; CLINICAL RESEARCH; CONTROLLED STUDY; DNA DETERMINATION; DNA PURIFICATION; GENE AMPLIFICATION; GENE MUTATION; GENETIC ANALYSIS; GENETIC PURITY; GENETIC TRANSFORMATION; HIGHER PLANT; MITOCHONDRIAL GENETICS; MITOCHONDRION; NONHUMAN; PLANT GENETICS; PLASTID; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PULSED FIELD GEL ELECTROPHORESIS; RELIABILITY; SENSITIVITY ANALYSIS; SHOOT REGENERATION; TOBACCO; WILD TYPE;

EMBRYOPHYTA; NICOTIANA TABACUM;

EID: 0013140529     PISSN: 01728083     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00294-003-0369-4     Document Type: Article

References (53)

1. Ayliffe MA, Timmis JN (1992) Tobacco nuclear DNA contains long tracts of homology to chloroplast DNA. Theor Appl Genet 85:229-238
2. Ayliffe MA, Scott NS, Timmis JN (1998) Analysis of plastid DNA-like sequences within the nuclear genomes of higher plants. Mol Biol Evol 15:738-745
3. Backert S, Dörfel P, Börner T (1995) Investigation of plant organellar DNAs by pulsed-field gel electrophoresis. Curr Genet 28:390-399
4. Baena-Gonzalez E, Gray JC, Tyystjarvi E, Aro EM, Mäenpää P (2001) Abnormal regulation of photosynthetic electron transport in a chloroplast ycf9 inactivation mutant. J Biol Chem 276:20795-20802
5. Bendich AJ (1987) Why do chloroplasts and mitochondria contain so many copies of their genome? Bio Essays 6:279-282
6. Bock R (2001) Transgenic plastids in basic research and plant biotechnology. J Mol Biol 312:425-438
7. Bogorad L (2000) Engineering chloroplasts: an alternative site for foreign genes, proteins, reactions and products. Trends Biotechnol 18:257-263
8. Boynton JE, Gillham NW, Harris EH, Hosler JP, Johnson AM, Jones AR, Randolph-Anderson BL, Robertson D, Klein TM, Shark KB, Sanford JC (1988) Chloroplast transformation in Chlamydomonas with high velocity microprojectiles. Science 240:1534-1538
9. Burrows PA, Sazanov LA, Svab Z, Maliga P, Nixon PJ (1998) Identification of a functional respiratory complex in chloroplasts through analysis of tobacco mutants containing disrupted plastid ndh genes. EMBO J 17:868-876
10. Daniell H, Khan MS, Allison L (2002) Milestones in chloroplast genetic engineering: an environmentally friendly era in biotechnology. Trends Plant Sci 7:84-91
11. Deng XW, Wing RA, Gruissem W (1989) The chloroplast genome exists in multimeric forms. Proc Natl Acad Sci USA 86:4156-4160
12. DeSantis-Maciossek G, Kofer W, Bock A, Schoch S, Maier RM, Wanner G, Rüdiger W, Koop HU, Herrmann RG (1999) Targeted disruption of the plastid polymerase genes rpoA, rpoB, rpoC1: molecular biology, biochemistry and ultrastructure. Plant J 18:477-489
13. Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13-15
14. Drescher A, Ruf S, Calsa T Jr, Carrer H, Bock R (2000) The two largest chloroplast genome-encoded reading frames of higher plants are essential genes. Plant J 22:97-104
15. Ellis J (1982) Promiscuous DNA - chloroplast genes inside plant mitochondria. Nature 299:678-679
16. Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspensions of soybean root cells. Exp Cell Res 50:151-158
17. Golds T, Maliga P, Koop HU (1993) Stable plastid transformation in PEG-treated protoplasts of Nicotiana tabacum. Biotechnology 11:95-97
18. Goldschmidt-Clermont M (1991) Transgenic expression of aminoglycoside adenyl transferase in the chloroplast: a selectable marker for site-directed transformation of Chlamydomonas. Nucleic Acids Res 19:4083-4089
19. Hedtke B, Legen J, Weihe A, Herrmann RG, Börner T (2002) Six active phage-type RNA polymerase genes in Nicotiana tabacum. Plant J 30:625-637
20. Herrmann RG (1982) The preparation of circular DNA from plastids. In: Edelman M, Hallick RB, Chua WH (eds) Methods in chloroplast molecular biology. Elsevier, Amsterdam, pp 259-280
21. Herrmann RG (1997) Eukaryotism, towards a new interpretation. In: Schenk HEA, Herrmann RG, Jeon KW, Müller NE, Schwemmler W (eds) Eukaryotism and symbiosis. Springer, Berlin Heidelberg New York, pp 73-118
22. Herrmann RG, Possingham JV (1980) Plastid DNA - the plastome. In: Reinert J (ed) Results and problems in cell differentiation, vol 10. Springer, Berlin Heidelberg New York, pp 45-96
23. 2 transport in the cyanobacterium in Synechocystis PCC 6803. Proc Natl Acad Sci USA 96:4006-4010
24. Katoh A, Sonoda M, Katoh H, Ogawa T (1996b) Absence of light-induced proton extrusion in a cotA-less mutant of Synechocystis sp. strain PCC 6803. J Bacteriol 178:5452-5455
25. Khan MS, Maliga P (1999) Fluorescent antibiotic resistance marker for tracking plastid transformants in higher plants. Nat Biotechnol 17:910-915
26. Kindle KL, Richards KL, Stern DB (1991) Engineering the chloroplast genome: techniques and capabilities for chloroplast transformation in Chlamydomonas reinhardtii. Proc Natl Acad Sci USA 88:1721-1725
27. Kleine M, Michalek W, Graner A, Herrmann RG, Jung C (1993) Construction of a barley (Hordeum vulgare L.) YAC library and isolation of a Hor1-specific clone. Mol Gen Genet 240:265-272
28. Kofer W, Koop HU, Wanner G, Steinmüller K (1998) Mutagenesis of the genes encoding subunits A, C, H, I, J and K of the plastid NAD(P)H-plastochinone-oxidoreductase in tobacco by polyethylene glycol-mediated plastome transformation. Mol Gen Genet 258:166-173
29. Koop HU, Kofer W (1995) Plastid transformation by polyethylene glycol treatment of protoplasts and regeneration of transplastomic tobacco plants. In: Potrykus I, Spangenberg G (eds) Gene transfer to plants. Springer, Berlin Heidelberg New York, pp 75-82
30. Koop HU, Steinmüller K, Wagner H, Rossler C, Eibl C, Sacher L (1996) Integration of foreign sequences into the tobacco plastome via polyethylene glycol-mediated plastom transformation. Planta 199:193-201
31. Koop HU, Kofer W, Steinmüller K (1998) Judging the homoplastomic state of plastid transformants - reply. Trends Plant Sci 3:377-378
32. Mäenpää P, Gonzales EB, Chen L, Khan MS, Gray JC, Aro EM (2000) The ycf9 (orf62) gene in the plant chloroplast genome encodes a hydrophobic protein of stromal thylakoid membranes. J Exp Bot 51:375-382
33. Maliga P (2002) Engineering the plastid genome of higher plants. Curr Opin Plant Biol 5:164-172
34. Maliga P, Nixon PJ (1998) Judging the homoplastomic state of plastid transformants. Trends Plant Sci 3:376-377
35. Moll B, Polsby L, Maliga P (1990) Streptomycin and lyncomycin resistances are selective plastid markers in cultured Nicotiana cells. Mol Gen Genet 221:245-250
36. Rochaix JD (1995) Chlamydomonas reinhardtii as the photosynthetic yeast. Annu Rev Genet 29:209-230
37. Rochaix JD (1997) Chloroplast reverse genetics: new insights into the function of plastid genes. Trends Plant Sci 2:419-425
38. Rolland N, Dorne AJ, Amoroso G, Sültemeyer DF, Joyard J, Rochaix JD (1997) Disruption of the plastid ycf10 open reading frame affects uptake of inorganic carbon in the chloroplast of Chlamydomonas. EMBO J 16:6713-6726
39. Ruf S, Biehler K, Bock R (2000) A small chloroplast-encoded protein as a novel architectural component of the light-harvesting antenna. J Cell Biol 149:369-378
40. Ruf S, Hermann M, Berger IJ, Carrer H, Bock R (2001) Stable genetic transformation of tomato plastids and expression of a foreign protein in fruit. Nat Biotechnol 9:870-875
41. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
42. Sasaki Y, Sekiguchi K, Nagano Y, Matsumo R (1993) Chloroplast envelope protein encoded by the chloroplast genome. FEBS Lett 316:93-98
43. Shinozaki K, Ohme M, Tanaka M, Wakasugi T, Hayashida N, Matsubayashi T, Zaita N, Chunwongse J, Obokata J, Yamaguchi-Shinozaki K, Ohto C, Torazawa K, Meng BY, Sugita M, Deno H, Kamogashira T, Yamada K, Kusuda J, Takaiwa F, Kato A, Tohdoh N, Shimada H, Sugiura M (1986). The complete nucleotide sequence of tobacco chloroplast genome: its gene organization and expression EMBO J 5:2043-2049
44. Sidkar SR, Serino G, Chaudhuri S, Maliga P (1998) Plastid transformation in Arabidopsis thaliana. Plant Cell Rep 18:20-24
45. Sidorov VA, Kasten D, Pang SZ, Haidukiewicz PTJ, Staub JM, Nehra NS (1999) Stable chloroplast transformation in potato: use of green fluorescent protein as a plastid marker. Plant J 19:209-216
46. Stern DB, Lonsdale DM (1982) Mitochondrial and chloroplast genomes of maize have a 12-kilobase DNA sequence in common. Nature 299:698-702
47. Svab Z, Maliga P (1993) High-frequency plastid transformation in tobacco by selection for a chimeric aadA gene. Proc Natl Acad Sci USA 90:913-917
48. Svab Z, Hajdukiewicz P, Maliga P (1990) Stable transformation of plastids in higher plants. Proc Natl Acad Sci USA 87: 8526-8530
49. Swiatek M, Kuras R, Sokolenko A, Higgs D, Olive J, Cinque G, Muller B, Eichacker LA, Stern DB, Bassi R, Herrmann RG, Wollman FA (2001) The chloroplast gene ycf9 encodes a photosystem II (PSII) core subunit, PsbZ, that participates in PSII supramolecular architecture. Plant Cell 13:1347-1367
50. The Arabidopsis Genome Initiative (2000) Analysis of the genome sequence of the flowering plant Arabidopsis thaliana. Nature 408:796-815
51. Timmis JN, Scott NS (1983) Spinach nuclear and chloroplast DNAs have homologous sequences. Nature 305:65-67
52. Unseld M, Marienfeld JR, Brandt P, Brennicke A (1997) The mitochondrial genome of Arabidopsis thaliana contains 57 genes in 366,924 nucleotides. Nat Genet 1:57-61
53. Willey DL, Gray LC (1990) An open reading frame encoding a putative haem-binding polypeptide is co-transcribed with the pea chloroplast gene for apocytochrome f. Plant Mol Biol 15:347-356