Crystal structure of an α1,4-N-acetylhexosaminyltransferase (EXTL2), a member of the exostosin gene family involved in heparan sulfate biosynthesis

Journal of Biological Chemistry

Volumn 278, Issue 16, 2003, Pages 14420-14428

  Pedersen, Lars C ^a,b   Dong, Jian ^a   Taniguchi, Fumiyasu ^c   Kitagawa, Hiroshi ^c   Krahn, Joe M ^a   Pedersen, Lee G ^b   Sugahara, Kazuyuki ^c   Negishi, Masahiko ^a  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

^b NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES   (United States)

^c KOBE PHARMACEUTICAL UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; CATALYSIS; CRYSTAL STRUCTURE; GENES; SUBSTRATES; X RAY ANALYSIS;

TRANSFER REACTIONS;

BIOCHEMISTRY;

ALPHA1,4 N ACETYLHEXOSAMINYLTRANSFERASE; ASPARAGINE; GLUCURONIC ACID; GLYCOSAMINOGLYCAN; HEPARAN SULFATE; N ACETYLGALACTOSAMINE; N ACETYLGLUCOSAMINE; UNCLASSIFIED DRUG; URIDINE DIPHOSPHATE; URIDINE DIPHOSPHATE N ACETYLGALACTOSAMINE; URIDINE DIPHOSPHATE N ACETYLGLUCOSAMINE; ARGININE; ASPARTIC ACID; EXTL2 PROTEIN, HUMAN; MEMBRANE PROTEIN; N ACETYLGALACTOSAMINYLTRANSFERASE; N ACETYLGLUCOSAMINYLTRANSFERASE; UDP N ACETYLGALACTOSAMINE (GLCUA GALNAC 4 SULFATE)(4) N ACETYLGALACTOSAMINYLTRANSFERASE; UDP-N-ACETYLGALACTOSAMINE (GLCUA-GALNAC-4-SULFATE)(4) N-ACETYLGALACTOSAMINYLTRANSFERASE;

ANIMAL CELL; ARTICLE; BIOSYNTHESIS; CATALYSIS; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVE SITE; ENZYME STRUCTURE; EXOSTOSIN GENE; GENE; MOUSE; MULTIGENE FAMILY; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; AMINO ACID SEQUENCE; ANIMAL; BINDING SITE; CELL STRAIN COS1; CHEMICAL STRUCTURE; CHEMISTRY; GENETIC TRANSFECTION; HYDROGEN BOND; METABOLISM; MOLECULAR GENETICS; PROTEIN CONFORMATION; PROTEIN TERTIARY STRUCTURE; SEQUENCE HOMOLOGY; X RAY CRYSTALLOGRAPHY;

ANIMALIA;

AMINO ACID SEQUENCE; ANIMALS; ARGININE; ASPARAGINE; ASPARTIC ACID; BINDING SITES; CATALYTIC DOMAIN; COS CELLS; CRYSTALLOGRAPHY, X-RAY; HEPARITIN SULFATE; HYDROGEN BONDING; MEMBRANE PROTEINS; MICE; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; N-ACETYLGALACTOSAMINYLTRANSFERASES; N-ACETYLGLUCOSAMINYLTRANSFERASES; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; SEQUENCE HOMOLOGY, AMINO ACID; TRANSFECTION; URIDINE DIPHOSPHATE N-ACETYLGLUCOSAMINE;

EID: 0012784535     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M210532200     Document Type: Article

References (27)

1. Lindahl, U., Kusche-Gullberg, M., and Kjellen, L. (1998) J. Biol. Chem. 273, 24979-24982
2. Perrimon, N., and Bernfield, M. (2000) Nature 404, 725-728
3. Forsberg, E., and Kjellen, L. (2001) J. Clin. Invest. 108, 175-180
4. Duncan, G. A., McCormick, G., and Tufaro, F. (2001) J. Clin. Invest. 108, 511-516
5. Senay, C., Lind, T., Muguruma, K., Tone, Y., Kitagawa, H., Sugahara, K., Lidholt, K., Lindahl, U., and Kusche-Gullberg, M. (2000) EMBO Rep. 1, 282-286
6. Wuyts, W., and Van Hul, W. (2000) Hum. Mutat. 15, 220-227
7. Lind, T., Tufaro, F., McCormick, C., Lindahl, U., and Lidholt, K. (1998) J. Biol. Chem. 273, 26265-26268
8. Kitagawa, H., Shimakawa, H., and Sugahara, K. (1999) J. Biol. Chem. 274, 13933-13937
9. Kim, B. T., Kitagawa, H., Tamura, J., Saito, T., Kusche-Gullberg, M., Lindahl, U., and Sugahara, K. (2001) Proc. Natl. Acad. Sci. U. S. A. 98, 7176-7181
10. Esko, J. D., and Zhang, L. (1996) Curr. Opin. Struct. Biol. 6, 663-670
11. Gastinel, L. N., Bignon, C., Misra, A. K., Hindsgaul, O., Shaper, J. H., and Joziasse, D. H. (2001) EMBO J. 20, 638-649
12. Persson, K., Ly, H. D., Dieckelmann, M., Wakarchuk, W. W., Withers, S. G., and Strynadka, N. C. (2001) Nat. Struct. Biol. 8, 166-175
13. Boix, E., Zhang, Y., Swaminathan, G. J., Brew, K., and Acharya, K. R. (2002) J. Biol. Chem. 277, 28310-28318
14. Otwinowski, Z., and Minor, V. (1997) Methods Enzymol. 276, 307-326
15. Brünger, A. T., Adams, P. D., Clore, G. M., DeLano, W. L., Gros, P., Grosse-Kunstleve, R. W., Jiang, J. S., Kuszewski, J., Nilges, M., Pannu, N. S., Read, R. J., Rice, L. M., Simonson, T., and Warren, G. L. (1998) Acta Crystallogr. Sect. D Biol. Crystallogr. 54, 905-921
16. Jones, T. A., Zou, J. Y., Cowan, S. W., and Kjeldgaard, M. (1991) Acta Crystallogr. Sect. A 47, 110-119
17. Laskowski, R. A., MacArthur, M. W., Moss, D. S., and Thornton, J. M. (1993) J. Appl. Crystallogr. 26, 283-291
18. Kitagawa, H., and Paulson, J. C. (1994) J. Biol. Chem. 269, 1394-1401
19. Fritz, T. A., Gabb, M. M., Wei, G., and Esko, J. D. (1994) J. Biol. Chem. 269, 28809-28814
20. Mizushima, S., and Nagata, S. (1990) Nucleic Acids Res. 18, 5322
21. Toth, G., Murphy, F. M., and Lovas, S. (2001) Protein Eng. 14, 543-547
22. Pedersen, L., Tsuchida, K., Kitagawa, H., Sugahara, K., Darden, T. A., and Negishi, M. (2000) J. Biol. Chem. 275, 34580-34585
23. Holm, L., and Sander, C. (1993) J. Mol. Biol. 233, 123-138
24. Pedersen, L. C., Darden, T. A., and Negishi, M, (2002) J. Biol. Chem. 277, 21869-21873
25. Kraulis, P. J. (1991) J. Appl. Crystallogr. 24, 946-950
26. Merritt, E. A., and Bacon, D. J. (1997) Methods Enzymol. 277, 505-524
27. Jeanmougin, F., Thompson, J. D., Gouy, M., Higgins, D. G., and Gibson, T. J. (1998) Trends Biochem. Sci. 23, 403-405