Purification and partial characterization of a dipeptidyl peptidase from prevotella intermedia

Oral Microbiology and Immunology

Volumn 18, Issue 3, 2003, Pages 196-198

  Shibata, Y ^a   Miwa, Y ^b   Hirai, K ^a   Fujimura, S ^a  

^a MATSUMOTO DENTAL UNIVERSITY   (Japan)

^b FUKUYAMA UNIVERSITY   (Japan)

Author keywords

Enzyme; Peptidase; Periodontopathogen; Prevotella intermedia

Indexed keywords

4 NITROANILINE; ALANINE; ANILIDE; ARGININE; BACTERIAL ENZYME; DIPEPTIDYL PEPTIDASE; DIPEPTIDYL PEPTIDASE IV; LYSINE; SERINE;

ARTICLE; CHROMATOGRAPHY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; HYDROLYSIS; ION EXCHANGE CHROMATOGRAPHY; MOLECULAR SIZE; NONHUMAN; PERIODONTITIS; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PREVOTELLA INTERMEDIA;

AMINO ACID SEQUENCE; ANTIGENS, CD26; BACTERIAL PROTEINS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYDROGEN-ION CONCENTRATION; MOLECULAR WEIGHT; PREVOTELLA INTERMEDIA; VIRULENCE FACTORS;

EID: 0012330604     PISSN: 09020055     EISSN: None     Source Type: Journal    
DOI: 10.1034/j.1399-302X.2003.00057.x     Document Type: Article

References (14)

1. Chich JF. X-Pro dipeptidyl-peptidase. In: Barret AJ, Rawlings ND, Woessner JF, eds. Handbook of proteolytic enzymes. London: Academic Press, 1998: 403-405.
2. Gazi MI, Cox SW, Clark DT, Eley BM. Comparison of host tissue and bacterial dipeptidyl peptidases in human gingival crevicular fluid by analytical isoelectric focusing. Arch Oral Biol 1995: 40: 731-736.
3. Kabashima T, Yoshida T, Ito K, Yoshimoto T. Cloning, sequencing, and expression of the dipeptidyl peptidase IV gene from Flavobacterium meningosepticum inEscherichia coli. Arch Biochem Biophys 1995: 320: 123-128.
4. Kabashima T, Ito K, Yoshimoto T. Dipeptidyl peptidase IV from Xanthomonas maltophilia: sequencing and expression of the enzyme gene and characterization of the expressed enzyme. J Biochem 1996: 120: 1111-1117.
5. Kenny AJ, Booth AG, George SG, Ingram J, Kershaw D, Wood EJ, Young AR. Dipeptidyl peptidase IV, a kidney brush-border serine peptidase. Biochem J 1976: 155: 169-182.
6. Kumagai Y, Konishi K, Gomi T, Yagishita H, Yajima A, Yoshikawa M. Enzymatic properties of dipeptidyl aminopeptidase IV produced by the periodontal pathogen Porphyromonas gingivalis and its participation in virulence. Infect Immun 2000: 68: 716-724.
7. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 1970: 227: 680-685.
8. Misumi Y, Ikehara Y. Dipeptidyl-peptidase IV. In: Barret AJ, Rawlings ND, Woessner JF, eds. Handbook of proteolytic enzymes. London: Academic Press, 1998: 378-382.
9. Miyauchi T, Hayakawa M, Abiko Y. Purification and characterization of glycylprolyl aminopeptidase from Bacteroides gingivalis. Oral Microbiol Immunol 1989: 4: 222-226.
10. Mouton C, Hamond PG, Slots J, Genco RJ. Serum antibodies to oral Bacteroides asaccharolyticus (Bacteroides gingivalis): relationship to age and periodontal disease. Infect Immun 1981: 31: 182-192.
11. Shibata Y, Fujimura S, Nakamura T. Purification and characterization of an elastolytic serine protease of Prevotella intermedia. Appl Environ Microbiol 1993: 59: 2107-2111.
12. Slots J. Subgingival microflora and periodontal disease. J Clin Periodontol 1979: 6: 351-382.
13. Slots J, Emrich LJ, Genco J, Rosling G. Relationship between some subgingival bacteria and periodotal pocket depth and gain or loss of periodontal attachment after treatment of adult periodontitis. J Clin Periodontal 1985: 12: 540-552.
14. White D, Mayrand D. Association of oral Bacteroides with gingivitis and adult periodontitis. J Periodont Res 1981: 16: 259-265.