Improved ligation-anchored PCR strategy for identification of 5' ends of transcripts

BioTechniques

Volumn 21, Issue 1, 1996, Pages

  Ansari Lari, M Ali ^a   Jones, Stephen N ^a   Timms, Kirsten M ^a   Gibbs, Richard A ^a  

^a BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COMPLEMENTARY DNA; MESSENGER RNA; PRIMER DNA;

ANIMAL CELL; ARTICLE; DIAGNOSTIC ACCURACY; DNA SEQUENCE; GENE ISOLATION; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION;

EID: 0011445960     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/96211bm06     Document Type: Article

References (4)

1. Barak, Y., E. Gottlieb, T. Juven-Gershon and M. Oren. 1994. Regulation of mdm2 expression by p53: alternative promoters produce transcripts with nonidentical translation potential. Genes Dev. 8:1739-1749.
2. Forhman, M.A., M.K. Dush and G.R. Martin. 1988. Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer. Proc. Natl. Acad. Sci. USA 85:8998-9002.
3. Sambrook, J., E.F. Fritsch and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
4. Troutt, A.B., M.G. McHeyzer-Williams, B. Pulendran and G.J.V. Nossal. 1992. Ligation-anchored PCR: a simple amplification technique with single-sided specificity. Proc. Natl. Acad. Sci. USA 89:9823-9825.