Removal of MAP4 from microtubules in vivo produces no observable phenotype at the cellular level

Journal of Cell Biology

Volumn 132, Issue 3, 1996, Pages 345-357

  Wang, Xiao Min ^a,c   Peloquin, John G ^a   Zhai, Ye ^a,d   Bulinski, J Chloe ^b   Borisy, Gary G ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

^b College of Physicians and Surgeons ^*  (United States)

^c STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

^d UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; MICROTUBULE ASSOCIATED PROTEIN; MICROTUBULE ASSOCIATED PROTEIN 4; NOCODAZOLE; POLYCLONAL ANTIBODY; TUBULIN;

ANIMAL CELL; ANTIGEN ANTIBODY COMPLEX; ARTICLE; CONTROLLED STUDY; EPITHELIUM CELL; FIBROBLAST; HUMAN; HUMAN CELL; MICROTUBULE ASSEMBLY; MITOSIS; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL;

ANIMALS; ANTIBODY SPECIFICITY; BRAIN; CELL LINE; CELLS, CULTURED; CLONING, MOLECULAR; ESCHERICHIA COLI; FIBROBLASTS; GLUTATHIONE TRANSFERASE; GOLGI APPARATUS; HAPLORHINI; HELA CELLS; HUMANS; IMMUNE SERA; MALE; MICROTUBULE-ASSOCIATED PROTEINS; MICROTUBULES; MITOCHONDRIA; NOCODAZOLE; ORGANELLES; PHENOTYPE; PROTEIN PROCESSING, POST-TRANSLATIONAL; RABBITS; RATS; RECOMBINANT FUSION PROTEINS; SKIN; SKIN PHYSIOLOGY; SWINE; TUBULIN;

EID: 0011214518     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.132.3.345     Document Type: Article

References (68)

1. r 190,000. J. Biol. Chem 265:13849-13855.
2. Aizawa, H., Y. Emori, A. Mori, H. Murofushi, H. Sakai, and, K. Suzuki. 1991 Functional analyses of the domain structure of microtubule-associated protein-U (MAP-U). J. Biol. Chem. 266:9841-9846.
3. Aizawa, H., M. Kamijo, Y. Ohba, A. Mori, K. Okuhara, H. Kawasaki, H. Murofushi, K. Suzuki, and H Yasuda 1991. Microtubule destabilization by cdc2/ H1 histone kinase: phosphorylation of a "Pro-rich region" in the microtubule-binding domain of MAP4. Biochem. & Biophy Res. Commun 179: 1620-1626
4. Allan, V. J., and T. E. Kreis. 1986. A microtubule-binding protein associated with membranes of the Golgi apparatus. J Cell Biol 103:2229-2239.
5. Andersen, S. S. L., B. Buendia, J. E. Dominguez, A. Sawyer, and E. Karsenti. 1994. Effect on microtubule dynamics of XMAP230, a microtubule-associated protein present in Xenopus Laevis eggs and dividing cells. J. Cell Biol 127:1289-1299
6. Ball, E. H., and S. J. Singer. 1982. Mitochondria are associated with microtubules and not with intermediate filaments in cultured fibroblasts Proc Natl Acad. Sci. USA. 79:123-126.
7. Barlow, S., M. L. Gonzalez-Garay, R. R. West, J. B Olmsted, and F. Cabral. 1994. Stable expression of heterologous microtubule-associated proteins in chinese hamster ovary cells evidence for differing roles of MAPs in miciotubule organization. J. Cell Biol 126:1017-1029.
8. Belmont, L D , A. A. Hyman, K. E. Sawin, and T J. Mitchison. 1990. Real-time visualization of cell cycle-dependent changes in microtubule dynamics in cytoplasmic extracts. Cell. 62:579-589
9. Borisy, G G., J. M. Marcum, J. B. Olmsted, D. B. Murphy, and K. A. Johnson. 1975. Purification of tubulin and associated high molecular weight proteins from porcine brain and characterization of microtubule assembly in vitro Ann. NY Acad. Sci. 253:107-132.
10. Bradford, M. M 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Anal. Biochem 72:248-254
11. Bulinski, J. C., and G. G. Borisy. 1979. Self-assembly of Hela tubulin and the identification of Hela microtubule-associated proteins. Proc. Natl. Acad. Sci USA. 76:293-297
12. Bulinski, J. C., and G. G. Borisy. 1980a. Immunofluorescence localization of Hela cell MAPs on microtubules in vitro and in vivo. J Cell Biol 87:792-801
13. Bulinski, J. C., and G G. Borisy. 1980b Widespread distribution of a 210,000 mol wt microtubule-associated protein in cells and tissue of primates. J. Cell Biol. 87:802-808.
14. Bulinski, J. C , and A. Bossler. 1994. Purification and characterization of ensconsin, a novel microtubule stabilizing protein. J. Cell Sci. 107:2839-2849.
15. Bulinski, J. C. 1994. MAP4. In Microtubules J. S Hyams and C. W. Lloyd, editors Wiley-Liss, New York. 167-182.
16. Caceres, A., and K. S. Kosik. 1990. Inhibition of neurite polarity by tau antisense oligonucleotides in primary cerebellar neurons. Nature (Lond.) 343: 461-463.
17. Cassimeris, L. U., R. A. Walker, and N. K. Pryer. 1988. Real-time observation of microtubule dynamic instability in living cells. J Cell Biol. 107:2223-2231.
18. r microtubule-associated protein 4 (MAP4) contains a domain homologous to the microtubule-binding domains of neuronal MAP2 and tau. J Cell Sci. 98:27-36.
19. Chapin, S. J., and J. C. Bulinski. 1992. Microrubule stabilization by assembly promoting microtubule-associated proteins: A repeat performance Cell Motil & Cytoskel. 23:236-243
20. Chapin, S. J., C M. Lue, M. T. Yu, and J C. Bulinski. 1995. Differential expression of alternatively spliced forms of MAP4: A repertoire of structurally different microtubule-binding domains Biochemistry. 34:2289-2301.
21. Chen, L. B. 1989. Fluorescent labeling of mitochondria. In Methods in Cell Biology. Yu-li Wang and D. Lansing Taylor, editors. Vol. 29. Harcourt Brace Jovanovich, Cleveland, OH. 103-123.
22. Dingus, J , R A Obar, J. S. Hyams, M. Goedert, and R. Vallee. 1991. Use of heat-stable microtubule-associated protein class-specific antibody to investigate the mechanism of microtubule binding. J. Biol. Chem. 266:18854-18860.
23. Dinsmore, J H , and F. Solomon. 1991. Inhibition of MAP2 expression affects both morphological and cell division phenotypes of neuronal differentiation. Cell. 64:817-826.
24. Drubin, D. G , and M. W. Kirschner. 1986. Tau protein function in living cells. J Cell Biol. 103:2739-2746.
25. Gundersen, G. G., S. Khawaja, and J. C. Bulinski. 1987. Post-polymerization detyrosination of alpha-tubulin: a mechanism for subcellular differentiation of microtubules. J. Cell Biol. 105:251-264.
26. Harada, A., K. Oguchi, S. Okabe, J. Kuno, S. Terada, T Ohshima, R Sato-Yoshitake, Y. Takei, T. Noda, and N Hirokawa 1994. Altered microtubule organization in small-calibre axons of mice lacking tau protein. Nature (Lond.). 369:488-491.
27. Hirokawa, N. 1994. Microtubule organization and dynamics dependent on microtubule-associated proteins. Curr Opin. Cell Biol 6:74-81.
28. Izant, J. G., J. A Weatherbee, and J. R. McIntosh. 1983. A microtubule-associated protein antigen unique to mitotic spindle microtubules in PtK1 cells J Cell Biol. 96:424-434.
29. Jockusch, B M , B. Zurk, R. Zahn, A. Westmeyer, and A Fuchtbauer. 1991 Antibodies against vertebrate microfilament proteins in the analysis of cellular motility and adhesion. J. Cell Sci. Suppl 14:41-47.
30. Kanai, Y., R Takemura, T Oshima, H. Mori, Y. Ihara, M. Yanagisawa, T. Masaki, and N. Hirokawa. 1989. Expression of multiple tau isoforms and microtubule bundle formation in fibroblasts transfected with a single tau cDNA. J. Cell Biol. 109:1173-1184.
31. Khawaja, S., G. G. Gundersen, and J. C. Bulinski 1988. Enhanced stability of microtubules enriched in detyrosinated tubulin is not a direct function of the detyrosination level. J. Cell Biol. 93:576-582.
32. Laemmli, U.K. 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (Lond.). 227:680-685.
33. Lee, G., N. Cowan, and M. Kirschner. 1988. The primary structure and heterogeneity of tau protein from mouse brain Science (Wash. DC). 239:285-288.
34. Lee, G., and S. L. Rook. 1992 Expression of tau protein in non-neuronal cells: microtubule binding and stabilization. J. Cell Sci. 101:227-237.
35. Lewis, S. A., D. Wang, and N. J Cowan. 1988 Microtubule-associated protein MAP2 shares a microtubule binding motif with tau protein Science (Wash. DC). 242:936-939.
36. Lews, S. A , I. E. Vanov, G. H. Lee, and N. J Cowan. 1989. Microtubule organization in dendrites and axons is determined by a short hydrophobic zipper in microtubule-associated proteins MAP2 and tau. Nature (Lond.). 342:498-505.
37. Lieuvin, A., J. Labbé, M. Dorée, and D. Job 1994 Intrinsic microtubule stability in interphase cells J. Cell Biol 124:985-996
38. Mangeat, P. H., and K Burridge 1984. Immunoprecipitation of nonerythrocyte spectrin within live cells following microinjection of specific antibodies: relation to cytoskeletal structures J Cell Biol 98:1363-1377.
39. Matus, A 1994 MAP2. In Microtubules. J S. Hyams and C. W. Lloyd, editors. Wiley-Liss, New York. 155-166.
40. Mitchison, T. J., and M. W. Kirschner. 1984. Dynamic instability of microtubule growth. Nature (Lond.). 312:237-247.
41. Nuckolls, G. H., L. H. Romer, and K. Burridge. 1992 Microinjection of antibodies against talin inhibits the spreading and migration of fibroblasts. J Cell Sci 102:753-762.
42. Olmsted J. B., D L. Stemple, W M Saxton, B W Neighbors, and J R. McIntosh 1989. Cell cycle-dependent changes in the dynamics of MAP2 and MAP4 in cultured cells. J. Cell Biol. 109:211-223.
43. Olmsted, J. B. 1991. Non-motor microtubule-associated proteins. Curr. Opin Cell Biol. 3:52-58
44. cdc2 kinase to microtubules and is a potential regulator of M-phase microtubule dynamics. J. Cell Biol. 128:849-862.
45. Pagano, R. E. 1989. A fluorescent derivative of ceramide: physical properties and use in studying the Golgi apparatus of animal cells. In Methods in Cell Biology. Y L. Wang and D. L. Taylor, editors. Vol. 29. Harcourt Brace Jovanovich, Cleveland, OH. 75-85.
46. Pereira, A , J. Doshen, E Tanaka, and L. S. B. Goldstein. 1992. Genetic analysis of a Drosophila microtubule-associated protein. J. Cell Biol. 116:377-383
47. Pierre, P., J. Scheel, J. E Rickard, and T. E Kreis. 1992. CLIP-170 links endocytic vesicles to microtubules. Cell 70:887-900.
48. Pierre, P., R Pepperkok, and T. E. Kreis 1994. Molecular characterization of two functional domains of CLIP-170 in vivo J. Cell Sci 107:1909-1920
49. Piperno, G., M. LeDizet, and X. J. Chang. 1987. Microtubules containing acetylated alpha-tubulin in mammalian cells in culture. J. Cell Biol. 104:289-302.
50. Sammak, P. J., and G. G. Borisy. 1988. Direct observation of microtubule dynamics in living cells. Nature (Lond.). 332:724-726.
51. Saxton, W. M., D. L. Stemple, R. J. Leslie, E. D Salmon, M. Zavortink, and J. R. McIntosh. 1984. Tubulin dynamics in cultured mammalian cells. J. Cell Biol. 99:2175-2186
52. Schulze, E., and M. Kirschner 1986. Microtubule dynamics in interphase cells. J. Cell Biol. 102:1020-1031.
53. Schulze, E., and M. Kirschner. 1987. Dynamic and stable populations of microtubules in cells. J. Cell Biol 104:277-288
54. Shiina, N., T. Moriguchi, E. Ohta, Y. Gotoh, and E. Nishida. 1992. Regulation of a major microtubule associated protein by MPF and MAP kinase. EMBO J 11:3977-3984.
55. Smith, D. B , and K. S. Johnson. 1988 Single-step purification of polypeptides expressed in Escherichia coli as fusion with glutathione S-transferase. Gene. 67:31-40.
56. Soltys, B. J., and G. G. Borisy. 1985. Polymerization of tubulin in vivo: direct evidence for assembly onto microtubule ends and from centrosomes. J. Cell Biol 100:1682-1689.
57. Towbin, H., T. Staehelin, and J. Gordon 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci. USA. 76:4350-4354.
58. Vallee, R B., and G. G. Borisy. 1978. The non-tubulin component of microtubule protein oligomers: effect on self-association and hydrodynamic properties J. Biol. Chem. 253:2834-2845.
59. Vandenbunder, B., and G. G. Borisy. 1986. Decoration of microtubules by fluorescently labeled microtubule-associated protein 2 (MAP2) does not interfere with their spatial organization and progress through mitosis in living fibroblasts. Cell Motil & Cytoskel 6:570-579.
60. Vandré, D. D., V. E. Centonze, J. G. Peloquin, R M. Tombes, and G. G. Borisy. 1991. Proteins of the mammalian mitotic spindle: phosphorylation/ dephosphorylation of MAP4 during mitosis. J. Cell Sci 98:577-588.
61. Verde, F., J. Labbe, M. Doree, and E Karsenti. 1990. Regulation of microtubule dynamics by cdc2 protein kinase in cell-free extracts of Xenopus eggs. Nature (Lond). 343:233-238.
62. Weatherbee, J. A., R. B. Luftig, and R. R. Weihing. 1980 Purification and reconstitution of Hela microtubules. Biochemistry. 19:4116-4123.
63. Webster, D. R., G. G. Gundersen, J. C Bulinski, and G. G. Borisy. 1987. Differential turnover of tyrosinated and detyrosinated microtubules. Proc. Natl. Acad Sci. USA. 94:9040-9044.
64. Webster, D. R., J. Wieland, K. Weber, and G. G. Borisy 1990 Detyrosination of alpha tubulin does not stabilize microtubules in vivo. J. Cell Biol. 111:113-122.
65. Welch, M. D., D. A Holtzman, and D. G. Drubin. 1994. The yeast actin cytoskeleton. Curr. Opin. Cell Biol. 6:110-119.
66. West, R R., K. M Tenbarge, and J. B. Olmsted. 1991 A model for microtubule-associated protein 4 structure. J Biol. Chem 266:21886-21896.
67. Zhai, Y , and G. G. Borisy. 1994. Quantitative determination of the proportion of microtubule polymer during the mitosis-interphase transition. J. Cell Sci. 107(4):881-890.
68. Zhai, Y , P. J. Kronebusch, and G. G Borisy. 1995. Kinetochore microtubules dynamics and the metaphase-anaphase transition. J. Cell Biol 131:721-734.