A method for rapid purification of antibody conjugates

American Biotechnology Laboratory

Volumn 14, Issue 7, 1996, Pages 49-50

  Blizzard, Charles D ^a   Garramone, Susan ^a   Gordon, Scott ^a   Gammell, Melissa ^a  

^a Middlesex Sciences Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0010612609     PISSN: 07493223     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (12)

1. Harlowe E, Lane D. Antibodies: a laboratory manual. New York: Cold Spring Harbor, 1988:321, 354.
2. Tijssen P. Practice and theory of enzyme immunoassays. North-Holland: Elsevier, 1993:221, 268.
3. Rothstein F. Differential precipitation of proteins. Protein purification process engineering. In: Harrison RG, ed. New York: Marcel Dekker Inc., 1994:150.
4. Ralston GB. Effects of "crowding" in protein solutions. J Chem Ed 1990; 67:857.
5. Polson A, Potgieter GM, Largler JF, Mear GEF, Jiubert FJ. The fractionation of protein mixtures by linear polymers of high molecular weight. Biochem Biophys Acta 1964; 82:463.
6. Scopes RK. Separation by precipitation. Protein purification principles and practice. 2nd ed. New York: Springer-Verlag, 1987:62.
7. Wilson MB, Nakane PK. In: Knapp W, Holubar H, Wick G, eds. Immunofluorescence and related techniques. North-Holland: Elsevier, 1978:215.
8. Goding JW. Conjugation of antibodies with fluorochromes: modifications to the standard methods. J Immunol Meth 1976; 13:217.
9. Bayer EA, Wilchek M. The use of avidin-biotin complexes as a tool in molecular biology. Meth Biochem Anal 1980; 26:1-45.
10. Farr AG, Nakane PK. Immunohistochemistry with enzyme labeled antibodies: a brief review. J Immunol Meth 1981; 47:131.
11. Avrameas S. Coupling of enzymes to proteins with glutaraldehyde. Use of the conjugates for the detection of antigens and antibodies. Immunochem 1969; 6:44.
12. Blizzard CD, Garramone S. Antibody purification by selective precipitation with polymers. Am Biotech Lab 1995; 13(3):71.