Association between antifol resistance in vitro and DHFR gene point mutation in Plasmodium falciparum isolates

Transactions of the Royal Society of Tropical Medicine and Hygiene

Volumn 90, Issue 2, 1996, Pages 181-182

  Eldin De Pécoulas, Philippe ^a,b   Basco, Leonardo K ^a,b   Le Bras, Jacques ^b   Mazabraud, André ^a  

^a CNRS   (France)

^b BICHAT HOSPITAL   (France)

Author keywords

Antifol resistance; DHFR gene mutation; Malaria; Plasmodium falciparum

Indexed keywords

CYCLOGUANIL; DIHYDROFOLATE REDUCTASE; FOLIC ACID ANTAGONIST; PYRIMETHAMINE; THYMIDYLATE SYNTHASE;

ARTICLE; BLOOD SAMPLING; CODON; CONTROLLED STUDY; DNA SEQUENCE; DRUG RESISTANCE; ERYTHROCYTE; HUMAN; HUMAN CELL; NONHUMAN; PLASMODIUM FALCIPARUM; POINT MUTATION; POLYMERASE CHAIN REACTION; STATISTICAL ANALYSIS;

EID: 0010600225     PISSN: 00359203     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0035-9203(96)90130-3     Document Type: Article

References (6)

1. Basco, L. K., Ramiliarisoa, O. & Le Bras, J. (1994). In vitro activity of pyrimethamine, cycloguanil, and other antimalarial drugs against African isolates and clones of Plasmodium falciparum. American Journal of Tropical Medicine and Hygiene, 50, 193-199.
2. Basco, L. K., Eldin de Pécoulas, P., Wilson, C. M., Le Bras, J. & Mazabraud, A. (1995). Point mutations in the dihydrofolate reductase-thymidylate synthase gene and pyrimethamine and cycloguanil resistance in Plasmodium falciparum. Molecular and Biochemical Parasitology, 69, 135-138.
3. Eldin de Pécoulas, P., Basco, L. K., Abdallah, B., Djé, M. K., Le Bras, J. & Mazabraud, A. (1995). Plasmodium falciparum: detection of antifolate resistance by mutation-specific restriction enzyme digestion. Experimental Parasitology, 80, 483-487.
4. Foote, S. J., Galatas, D. & Cowman, A. F. (1990). Amino acids in the dihydrofolate reductase-thymidylate synthase gene of Plasmodium falciparum involved in cycloguanil resistance differ from those involved in pyrimethamine resistance. Proceedings of the National Academy of Sciences of the USA, 87, 3014-3017.
5. Peterson, D. S., Milhous, W. K. & Wellems, T. E. (1990). Molecular basis of differential resistance to cycloguanil and pyrimethamine in Plasmodium falciparum malaria. Proceedings of the National Academy of Sciences of the USA, 87, 3018-3022.
6. Peterson, D. S., Di Santi, S. M., Povoa, M., Calvosa, V. S., do Rosario, V. E. & Wellems, T. E. (1991). Prevalence of the dihydrofolate reductase Asn-108 mutation as the basis for pyrimethamine-resistant falciparum malaria in the Brazilian Amazon. American Journal of Tropical Medicine and Hygiene, 45, 492-497.