A high sensitivity nitrate reductase assay and its application to vertically migrating Rhizosolenia mats

Aquatic Microbial Ecology

Volumn 12, Issue 1, 1997, Pages 95-104

  Joseph, Leanne ^a   Villareal, Tracy A ^a   Lipschultz, Fred ^b  

^a UNIVERSITY OF MASSACHUSETTS BOSTON   (United States)

^b BERMUDA INSTITUTE OF OCEAN SCIENCES   (Bermuda)

Author keywords

Chemiluminescence; Diatom; Nitrate reductase; Rhizosolenia mat; Vertical migration

Indexed keywords

EID: 0010315310     PISSN: 09483055     EISSN: None     Source Type: Journal    
DOI: 10.3354/ame012095     Document Type: Article

References (38)

1. Alldredge AL, Silver MW (1982) Abundance and production rates of floating diatom mats (Rhizosolenia castracanei and R. imbricata var. shrubsolei) in the eastern Pacific Ocean. Mar Biol 66:83-88
2. Ballek RW, Swift E (1986) Nutrient- and light-mediated buoyancy control of the oceanic non-motile dinoflagellate Pyrocystis noctiluca Murray ex Haeckel (1890). J Exp Mar Biol Ecol 101:175-192
3. Beevers L, Hageman RH (1969) Nitrate reduction in higher plants. Annu Rev Plant Physiol 20:495-522
4. Berges JA, Harrison PJ (1995) Nitrate reductase activity quantitatively predicts the rate of nitrate incorporation under steady state light limitation: a revised assay and characterization of the enzyme in three species of marine phytoplankton. Limnol Oceanogr 40:82-93
5. Blasco D, MacIsaac JJ, Packard TT, Dugdale RC (1984) Relationship between nitrate reductase and nitrate uptake in phytoplankton in the Peru upwelling region. Limnol Oceanogr 29:275-286
6. Carpenter EJ, Harbison GR, Madin LP, Swanberg NR, Biggs DC, Hulburt EM, McAlister VL, McCarthy JJ (1977) Rhizosolenia mats. Limnol Oceanogr 22:739-741
7. Chisholm SW (1992) Phytoplankton size. In: Falkowski PG, Woodhead AD (eds) Primary production and biogeochemical cycles in the sea. Plenum Press, New York, p 213-237
8. Collos Y, Lewin J (1974) Blooms of surf-zone diatoms along the coast of the Olympic Peninsula, Washington. IV. Nitrate reductase activity in natural populations and laboratory cultures of Chaetoceros armatum and Asterionella socialis. Mar Biol 25:213-221
9. Collos Y, Slawyk G (1976) Significance of cellular nitrate content in natural populations of marine phytoplankton growing in shipboard cultures. Mar Biol 34:27-32
10. Collos Y, Slawyk G (1977) Nitrate reductase activity as a function of in situ nitrate uptake and environmental factors of euphotic zone profiles. J Exp Mar Biol Ecol 29: 119-130
11. Cullen JJ, Zhu M, Davis RF, Pierson DC (1985) Vertical migration, carbohydrate synthesis, and nocturnal nitrate uptake during growth of Heterocapsa niei in a laboratory water column. In: Anderson DM, White AW, Baden DG (eds) Toxic dinoflagellates. Elsevier Science Publishing Co, New York, p 189-194
12. Dortch Q, Ahmed SI, Packard TT (1979) Nitrate reductase and glutamate dehydrogenase activities in Skeletonema costatum as measures of nitrogen assimilation rates. J Plankton Res 1:169-186
13. Dunn-Coleman NS, Smarrelli J Jr, Garrett RH (1984) Nitrate assimilation in eukaryotic cells. In: Danielli JF (ed) International review of cytology, Vol 92. Academic Press, New York, p 1-50
14. Eppley RW (1978) Nitrate reductase in marine phytoplankton. In: Hellebust JA, Craigie JS (eds) Handbook of phycological methods. Cambridge University Press, Cambridge, p 217-223
15. Eppley RW, Coatsworth JL, Solórzano L (1969) Studies of nitrate reductase in marine phytoplankton. Limnol Oceanogr 14:194-205
16. Everest SA, Hipkin CR, Syrett PJ (1984) The effect of phosphate and flavin adenine dinucleotide on nitrate reductase activity of some unicellular marine algae. J Exp Mar Biol Ecol 76:263-275
17. Gao Y, Smith GJ, Alberte RS (1992) Light regulation of nitrate reductase in Ulva fenestrata (Chlorophyceae). I. Influence of light regimes on nitrate reductase activity. Mar Biol 112: 691-696
18. Gao Y, Smith GJ, Alberte RS (1993) Nitrate reductase from the marine diatom Skeletonema costatum. Biochemical and immunological characterization. Plant Physiol 103: 1437-1445
19. Garside C (1982) A chemiluminescent technique for the determination of nanomolar concentrations of nitrate and nitrite in seawater. Mar Chem 11:159-167
20. Guerrero MG, Vega JM, Losada M (1981) The assimilatory nitrate-reducing system and its regulation. Annu Rev Plant Physiol 32:169-204
21. Hersey RL, Swift E (1976) Nitrate reductase activity of Amphidinium carteri and Cachonina niei (Dinophyceae) in batch culture: diel periodicity and effects of light intensity and ammonia. J Phycol 12:36-44
22. Hochman A, Nissany A, Wynne D, Kaplan B, Berman T (1986) Nitrate reductase: an improved assay method for phytoplankton. J Plankton Res 8:385-392
23. Kieber RJ, Seaton PJ (1995) Determination of subnanomolar concentrations of nitrite in natural waters. Analyt Chem 67:3261-3264
24. Martinez R, Packard TT, Blasco D (1987) Light effects and diel variations of nitrate reductase activity in phytoplankton from the northwest Africa upwelling region. Deep Sea Res 34:741-753
25. McCarthy JJ, Carpenter EJ (1983) Nitrogen cycling in nearsurface waters of the open ocean. In: Carpenter EJ, Capone DG (eds) Nitrogen in the marine environment. Academic Press, New York, p 487-512
26. Packard TT (1973) The light dependence of nitrate reductase in marine phytoplankton. Limnol Oceanogr 18:466-469
27. Packard TT, Blasco D (1974) Nitrate reductase activity in upwelling regions. 2. Ammonia and light dependence. Tethys 6:269-280
28. Ramalho CB, Hastings JW, Colepicolo P (1995) Circadian oscillation of nitrate reductase activity in Gonyaulax polyedra is due to changes in cellular protein levels. Plant Physiol 107:225-231
29. Ryther JH, Dunstan WM (1971) Nitrogen, phosphorus, and eutrophication in the coastal marine environment. Science 171:1008-1013
30. Scholl RL, Harper JE, Hageman RH (1974) Improvements of the nitrite color development in assays of nitrate reductase by phenazine methosulfate and zinc acetate. Plant Physiol 53:825-828
31. Slawyk G, Collos Y (1976) An automated assay for the determination of nitrate reductase in marine phytoplankton. Mar Biol 34:23-26
32. Smayda TJ (1978) From phytoplankters to biomass. In: Sourma A (ed) Monographs on oceanographic methodology, Vol 6, Phytoplankton manual. UNESCO, Paris, p 273-279
33. Venrick EL (1969) The distribution and ecology of oceanic diatoms in the North Pacific. PhD dissertation, University of California, San Diego
34. Villareal TA, Altabet MA, Culver-Rymsza K (1993) Nitrogen transport by vertically migrating diatoms mats in the North Pacific Ocean. Nature 363:709-712
35. Villareal TA, Carpenter EJ (1989) Nitrogen fixation, suspension characteristics, and chemical composition of Rhizosolenia mats in the central North Pacific Gyre. Biol Oceanogr 6:327-345
36. Villareal TA, Lipschultz F (1995) Internal nitrate concentrations in single cells of large phytoplankton from the Sargasso Sea. J Phycol 31:689-696
37. 3 fluxes in the central North Pacific gyre. J Plankton Res 18:1103-1121
38. Welschmeyer NA (1994) Fluorometric analysis of chlorophyll a in the presence of chlorophyll b and pheopigments. Limnol Oceanogr 39:1985-1992