Acta botanica sinica characteristics and crystallization of nitrogenase MoFe protein from a nif Z deleted strain of Azotobacter vinelandii

Acta Botanica Sinica

Volumn 41, Issue 1, 1999, Pages 71-74

  Wang, Dao Yong ^a   Wang, Yao Ping ^b   Huang, Ju Fu ^a   Wang, Zhi Ping ^c   Zhong, Ze Pu ^a   Li, Jia Ge ^a  

^a INSTITUTE OF BOTANY   (China)

^b INSTITUTE OF BIOPHYSICS   (China)

^c ZHEJIANG UNIVERSITY   (China)

Author keywords

nif Z MoFe protein; Azotobacter vinelandii mutant; Crystallization; Structure and function

Indexed keywords

EID: 0009848225     PISSN: 05777496     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (11)

1. Ludden P W. Nif gene products and their roles in nitrogen fixation. In: Palacios R, Mora J, Newton W E eds. New Horizons in Nitrogen Fixation. Netherlands: Kluwer Academic Publishers, 1993. 101-104
2. Kim J, Rees D C. Structural models for the metal centers in the nitrogenase molydenum-iron protein. Science, 1992,257:1677 -1682
3. Shal V K, Allen J R, Spangle N J, Ludden P W. In vitro synthesis of the iron-molybdenum cofacter of nitrogenase. Purification and characterization of Nif B cofacter, the product of Nif B protein. J Biol Chem, 1994,269:1154-1158
4. Zhong Z-P, Hu C-Z, Wang J-W, Huang J-F, Luo A-L, Li J-G. Purification and properties of nitrogenase MoFe protein from a Nif Z deletion strain of Azotobacter vinelandii. Acta Bot Sin, 1996,38:605-611 (in Chinese)
5. Burgess B K,Jocobs D B, Stiefel E I. Large scale purification of high activity Azotobacter vinelandii nitrogenase. Biochem Biophys Acta, 1980,614:196-209
6. Gornall A G, Bardawill C J, Dauid M M. Determination of serum proteins by means of the biuret reaction. J Biol Chem, 1949,177:751-766
7. 2 reduction by vanadium nitrogenase. Biochem J, 1988,256:429-432
8. Blanchard C Z, Hales B J. Isolation of two forms of the nitrogenase VFe protein from Azotobacter vinelandii. Biochemistry, 1996,35:472-478
9. Stephens P J, Mchenna C E, Smith B E, Nguyen H T, Mchenna M C, Thomson A J, Devlin F, Jones J B. Circular dichroism and magnetic circular dichroism of nitrogenase. Pro Natl Acad Sci, 1979,76:2585-2589
10. Drenth J, Helliwell J R, Iittke W. The crystal growth of biological materials. In: Walter H V ed. Fluid Science and Material Science in Space. Beijing: Chinese Science and Technology Press, 1991. 358-377
11. Huang J-F, Wang D-Y, Wang Z-P, Gu S-R, Li J-G. Effect of PEG and salt on crystallization of bacterioferritin and nitrogenase MoFe protein from Azotobacter vinelandii. Acta Bot Sin, 1998,40: 880-882 (in Chinese)