Purification and characterization of the recombinant Bacillus pasteurii urease overexpressed in Escherichia coli

Journal of Microbiology and Biotechnology

Volumn 9, Issue 3, 1999, Pages 255-259

  Shin, In Seon ^a   Lee, Mann Hyung ^a  

^a Catholic University of Daegu   (South Korea)

Author keywords

Bacillus pasteurii; Nickel; Sporosarcina ureae; Urease

Indexed keywords

UREASE;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GENE; ENZYME PURIFICATION; ESCHERICHIA COLI; SOIL MICROFLORA; SPOROSARCINA PASTEURII;

BACILLUS PASTEURII; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; PROKARYOTA; SPOROSARCINA PASTEURII; SPOROSARCINA UREAE;

EID: 0009589463     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (16)

1. Andrews, R. K., R. L. Blakeley, and B. Zerner. 1988. Urease-a Ni(II) metalloenzyme, pp. 141-165. In J. R. Lancaster, Jr. (ed.), The Bioinorganic Chemistry of Nickel, VCH Pub., Inc., New York, U.S.A.
2. Ben-Bassat, A., A. K. Bauer, S.-Y. Chang, K. Myambo, A. Moosman, and S. Chang. 1987. Processing of the initiation methionine from proteins: Properties of the Escherichia coli methionine aminopeptidase and its gene structure. J. Bacteriol. 169: 751-757.
3. Christians S. and H. Kaltwasser. 1986. Nickel contents of urease from Bacillus pasteurii. Arch. Microbiol. 145: 51-55.
4. Gualerzi, C. O. and C. L. Pon. 1990. Initiation of mRNA translation in prokaryotes. Biochemistry 29: 5881-5889.
5. Laemmli, U. K. 1970. Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature (London) 227: 680-685.
6. Larson, A. D. and R. E. Kallio. 1954. Purification and properties of bacterial urease. J. Bacteriol. 68: 67-73.
7. Lee, E.-T. and S.-D. Kim. 1992. Purification and enzymatic characteristics of the Bacillus pasteurii urease expressed in Escherichia coli. Kor. J. Appl. Microbiol. Biotechnol. 20: 519-526.
8. Lee, J. H., S. D. Kim, and M. H. Lee. 1996. Purification of the three-subunit, recombinant Bacillus pasteurii urease expressed in Escherichia coli. J. Microbiol. Biotechnol. 6: 26-29.
9. Lee, M. H., S. B. Mulrooney, M. J. Renner, Y. Markowicz, and R. P. Hausinger. 1992. Klebsiella aerogenes urease gene cluster: sequence of ureD and demonstration that four accessory genes (ureD, ureE, ureF, and ureG) are involved in nickel metallocenter biosynthesis. J. Bacteriol. 174: 4324-4330.
10. Lowry, O. H., N. J. Rosebrough, A. J. Farr, and R. J. Randall. 1951. Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-275.
11. McCoy, D. D., A. Cetin, and R. P. Hausinger. 1992. Characterization of urease from Sporosarcina ureae. Arch. Mirobiol. 157: 411-416.
12. Mobley, H. L. T., M. D. Island, and R. P. Hausinger. 1995. Molecular biology of microbial ureases. Microbiol. Rev. 59: 451-480.
13. Mobley, H. L. T. and R. P. Hausinger. 1989. Microbial ureases: Significance, regulation, and molecular characterization. Microbiol. Rev. 53: 85-108.
14. Pechman K. J., B. J. Lewis, and C. R. Woese. 1976. Phylogenetic status of Sporosarcina ureae. Int. J. Systemat. Bacteriol. 26: 305-310.
15. Weatherburn, M. W. 1967. Phenol-hypochlorite reaction for determination of ammonia. Anal. Chem. 39: 971-974.
16. You, J. H., B. H. Song, J. H. Kim, M. H. Lee, and S. D. Kim. 1995. Genetic organization and nucleotide sequencing of the ure gene cluster in Bacillus pasteurii. Mol. Cells 5: 359-369.