The crystal structure of NADPH:ferredoxin reductase from Azotobacter vinelandii

Protein Science

Volumn 7, Issue 12, 1998, Pages 2541-2549

  Sridhar Prasad, G ^a   Kresge, N ^a   Muhlberg, A B ^a   Shaw, A ^a,c   Jung, Y S ^b   Burgess, B K ^b   Stout, C D ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c GENENCOR INTERNATIONAL INC   (United States)

Author keywords

Crystal structure; FAD; Ferredoxin reductase; NADPH

Indexed keywords

FERREDOXIN NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE REDUCTASE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE;

ARTICLE; AZOTOBACTER VINELANDII; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME STRUCTURE; NONHUMAN; OXIDATIVE STRESS; PRIORITY JOURNAL;

ANABAENA; AZOTOBACTER VINELANDII; BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; SPINACIA OLERACEA;

EID: 0009435931     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560071207     Document Type: Article

References (32)

1. + reductase: Activation of E. coli anaerobic ribonucleotide reduction, cloning of the gene (fpr), and overexpression of the protein. J Bacteriol 175:1590-1595.
2. Brünger AT. 1992. Free R value: A novel statistical quantity for assessing the accuracy of crystal structures. Nature 355:472-475.
3. Brünger AT, Karplus M, Petsko GA. 1989. Crystallographic refinement by simulated annealing: Application to crambin. Acta Crystallogr A45:50-61.
4. Bruns CM, Karplus AP. 1995. Refined crystal structure of spinach ferredoxin reductase at 1.7 Å resolution: Oxidized, reduced and 2′-phospho-5′-AMP bound states. J Mol Biol 247:125-145.
5. Correll CC, Batie CJ, Ballou DP, Ludwig ML. 1992. Phthalate dioxygenase reductase: A modular structure for electron transfer from pyridine nucleotides to [2Fe-2S]. Science 258:1604-1610.
6. Correll CC, Ludwig ML, Bruns CM, Karplus PA. 1993. Structural prototypes for an extended family of flavoprotein reductases: Comparison of phthalate dioxygenase reductase with ferredoxin reductase and ferredoxin. Protein Sci 2:2112-2133.
7. Cowtan K. 1994. The CCP4 suite: Programs for protein crystallography. Acta Crystallogr D50:760-763.
8. Diller TC, Shaw A, Isas JM, Burgess BK, Stout CD. 1994. Diffraction quality crystals of protein X from Azotobacter vinelandii. J Mol Biol 247:620-621.
9. Howard AJ, Nielsen C, Xuong NH. 1985. Software for a diffractometer with multiwire area detector. Methods Enzymol 114A:452-472
10. + reductase for site-specific ferredoxin mutants. Biochemistry 36:11100-11117
11. Iismaa SE, Vasquez AE, Jensen GM, Stephens PJ, Butt JN, Armstrong FA, Burgess BK. 1991. Site directed mutagenesis of Azotobacter vinelandii ferredoxin I: Changes in [4Fe-4S] cluster reduction potential and reactivity. J Biol Chem 266:21563-21571.
12. Ingelman M, Bianchi V, Eklund H. 1997. The three-dimensional structure of flavodoxin reductase from Eschericha coli at 1.7 Å resolution. J Mol Biol 268:147-157.
13. - strains of Azotobacler vinelandii. J Biol Chem 269:19404-19409.
14. Isas JM, Yannone SM, Burgess BK. 1995. Azotobacter vinelandii NADPR: Ferredoxin reductase cloning, sequencing and overexpression. J Biol Chem 270:21258-21263.
15. + reductase: Prototype for structurally novel flavoenzyme family. Science 251:60-66.
16. Kraulis PJ. 1991. Molscript: A program to produce both detailed and schematic plots of protein structures. J Appl Cryst 24:946-950.
17. Leslie AGW. 1994. The CCP4 suite: Programs for protein crystallography. Acta Crytallogr D50:760-763.
18. Liochev SI, Hausladen A, Beyer WF, Fridovich I. 1994. NAPH:ferredoxin oxidoreductase acts as a paraquat diaphorase and is a member of the soxRS regulon. Proc Natl Acad Sci 91:1328-1331.
19. Lu G, Campbell WH, Schneider G, Lindqvist Y. 1994. Crystal structure of the FAD-containing fragment of corn nitrate reductase at 2.5 Å resolution: Relationship to other flavoprotein reductases. Structure 2:809-821.
20. Lu G, Lindqvist Y, Schneider G, Dwivedi U, Campbell W. 1995. Structural studies on corn nitrate reductase: Refined structure of the cytochrome b reductase fragment at 2.5 Å, its ADP complex and an active-site mutant and modeling of the cytochrome b domain. J Mol Biol 248:931-948.
21. McRee DE. 1992. A visual protein crystallographic software system for XII/ Xview. J Mol Graphics 10:44-47.
22. Merritt EA, Murphy MEP. 1994. Raster 3D Version 2.0. A program for photorealistic molecular graphics. Acta Cryst D50:869-873.
23. Morgan TV, Lundell DJ, Burgess BK. 1988. Azotobacter vinelandii ferrodoxin I: Cloning, sequencing, and mutant analysis. J Biol Chem 263:1370-1375.
24. Murzin AG. 1998. Probable circular permutation in the flavin-binding domain. Nat Struct Biol 5:101.
25. Nishida H, Inaka K, Yamanaka M, Kaida S, Kobayashi K, Miki K. 1995. Crystal structure of NADH-cytochrome b5 reductase from pig liver at 2.4 Å resolution. Biochemistry 34:2763-2767.
26. Nishida H, Miki K. 1996. Electrostatic properties deduced from refined structures of NADH-cytochrome b5 reductase and the other flavin-dependent reductases: Pyridine nucleotide-binding and interaction with an electron-transfer partner. Proteins Struct Fund Genet 26:32-41.
27. Read RJ. 1986. Improved Fourier coefficients for maps using phases from partial structures with errors. Acta Cryst A42:140-149.
28. + binding at 2.25 Å resolution. J Mol Biol 263: 20-39.
29. Stout CD. 1989. Refinement of the 7 Fe ferredoxin from Azotobacter vinelandii at 1.9 Å resolution. J Mol Biol 205:545-555.
30. Yannone SM, Burgess BK. 1997. Identification of a palindromic sequence that is responsible for the up-regulation of NADPH-ferredoxin reductase in a ferredoxin I deletion strain of Azotobacter vinelandii. J Biol Chem 272:14454-14458.
31. Yannone ST, Burgess BK. 1998. The seven iron FdI from Azotobacter vinelandii regulates the expression of NADPH: Ferredoxin reductase via an oxidative stress response. J Biol Inorg Chem. Forthcoming.
32. Wang M, Roberts DL, Paschke R, Shea TM, Masters BSS, Kim JJP. 1997. Three dimensional structure of NADPH-cytochrome P450 reductase: Prototype for FMN and FAD containing enzymes. Proc Natl Acad Sci 94:8411-8416.