Practical procedures to measure yeast viability and vitality prior to pitching

Journal of the American Society of Brewing Chemists

Volumn 56, Issue 1, 1998, Pages 1-6

  Mochaba, F ^a   O'Connor Cox, E S C ^a   Axcell, B C ^a  

^a S African Breweries Beer Division   (South Africa)

Author keywords

Magnesium release test; PH; Pitching procedure; Protease test; Vitality; Yeast viability

Indexed keywords

EID: 0009146932     PISSN: 03610470     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (18)

1. American Society of Brewing Chemists. Methods of Analysis, 8th Ed. The Society, St. Paul, MN, 1992.
2. Austin, G. D., Watson, R. W. J., and D'Amore, T. Studies of on-line viable yeast biomass with a capacitance biomass monitor. Biotechnol. Bioeng. 43:337-341, 1994.
3. Axcell, B., Kruger, L., and Allan, G. Some investigative studies with yeast foods. Proc. Inst. Brew. (Asia & Pacific Sect.) 20:164, 171, 1988.
4. Axcell, B. C., and O'Connor-Cox, E. S. C. The concept of yeast vitality: an alternative approach. Proc. Inst. Brew. (Asia & Pacific Sect.) 24:64-71, 1996.
5. Boulton, C. A., Maryan, P. S., Loveridge, D., and Kell, D. B. The application of a novel biomass sensor to the control of yeast pitching rate. Proc. Eur. Brew. Conv. 22:653-661, 1989.
6. Boulton, C. A., and Quain, D. E. Yeast, oxygen and the control of brewery fermentations. Proc. Eur. Brew. Conv. 21:401-408, 1987.
7. Cantrell, I. C., and Anderson, R. S. Yeast performance in production fermentations. Proc. Eur. Brew. Conv. 19:481-488, 1983.
8. Carvell, J. Automatic yeast pitching control using a yeast sensor. The Brewer 80:57-59, 1994.
9. Chapman, A. G., and Atkinson, D. E. Adenine nucleotide concentrations and turnover rates. Their correlation with biological activity in bacteria and yeast. Adv. Microbiol. Phys. 15:253-306, 1977.
10. Dufour, J. P., and Goffeau, A. Active transport in yeast cells during fermentation: What is the driving force of cellular active transport in yeast and how is this driving force created? Proc. Eur. Brew. Conv. 21:369-376, 1987.
11. European Brewery Convention. Analytica. Brauerei-und-Getranke-Rundschau, Zurich, Switzerland, 4th Ed., 1987.
12. Hutter, K.-J. Biomonitoring of yeasts in production - A concept for on-line monitoring of yeast during propagation, fermentation and maturation. Brauwelt Int. 15:116-123, 1997.
13. Hysert, D. W., and Morrison, N. M. Studies on ATP, ADP, and AMP concentrations in yeast and beer. J. Am. Soc. Brew. Chem. 35:160-167, 1977.
14. Imai, T., Nakajima, I., and Ohno, T. Development of a new method for evaluation of yeast vitality by measuring intracellular pH. J. Am. Soc. Brew. Chem. 52:5-8, 1994.
15. Institute of Brewing. Recommended Methods of Analysis, Microbiology 9, Methylene blue staining method. The Institute, London, 1982.
16. Jones, R. P. Measures of yeast death and deactivation and their meaning. Part I. Proc. Biochem. 22:118-128, 1987.
17. Kara, B. V., Simpson, W. J., and Hammond, J. R. M. Prediction of the fermentation performance of brewing yeast with the acidification power test. J. Inst. Brew. 94:153-158, 1988.
18. King, L. M., Schisler, D. O., and Ruocco, J. J. Epifluorescent method for detection of non-viable yeast. J. Am. Soc. Brew. Chem. 39:52-55, 1981.