Urea and heat unfolding of cold-adapted Atlantic Cod (Gadus morhud) trypsin and bovine trypsin

Journal of the Science of Food and Agriculture

Volumn 70, Issue 1, 1996, Pages 1-10

  Amiza, Mat Amin ^a   Apenten, Richard K Owusu ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

Author keywords

Bovine trypsin; Fish trypsin; Heat stability; Thermal unfolding; Urea unfolding

Indexed keywords

EID: 0008977913     PISSN: 00225142     EISSN: None     Source Type: Journal    
DOI: 10.1002/(sici)1097-0010(199601)70:1<1::aid-jsfa458>3.3.co;2-6     Document Type: Article

References (10)

1. Amelunxen R E, Murdock A L 1978 In Microbial Life in Extreme Environments, ed Kushner D J Academic Press, New York, USA, pp 217-278.
2. Amiza M A, Owusu Apenten R K 1994 Thermal inactivation for alkaline proteinases from North Sea cod (Gadus morhua) and bovine α-chymotrypsin. J Sci Food Agric 66 389-391.
3. Asgeirsson B, Bjarnason J B 1989 Structural and kinetic properties of chymotrypsin from Atlantic cod (Gadus morhua). Comparsion with bovine chymotrypsin. Comp Biochem Physiol 99B 327-335.
4. Asgeirsson B, Fox J W, Bjarnason J B 1989 Purification and characterisation of trypsin from the poikilotherm Gadus morhua. Eur J Biochem 180 85-94.
5. Aune K C, Tanford C 1969 Thermodynamics of the denaturation of lysozyme by guanidine hydrochloride. II. Dependence on denaturant concentration at 25°C. Biochemistry 8 4586-4590.
6. Becktel W J, Schellman JA 1987 Protein stability curves. Biopolymers 26 1859-1877.
7. Bjarnason J B, Asgeirsson B 1993 Psychrophilic proteolytic enzymes from Atlantic cod: their characteristics and applications. Genetic Engr Biotechnol 13 31-39.
8. Blumenfeld L A 1981 Problems in Biological Physics. Springer-Verlag, Berlin, Germany, pp 49-91.
9. Brand L, Witholt B 1967 Fluorescence measurements. Meth Enzymol 11 776-856.
10. Brandt J F 1964 The thermodynamics of protein denaturation 1. The denaturation of chymotrypsinogen. J Am Chem Soc 864291-4301.