Isolation of porcine pancreatic islets for xenotransplantation studies: Effects of low collagenase digestion temperatures

Xenotransplantation

Volumn 4, Issue 1, 1997, Pages 56-61

  Sun, Yilu ^a   Zhou, Daobiao ^a   Vacek, Ivan ^a   Sun, Anthony M ^a  

^a UNIVERSITY OF TORONTO   (Canada)

Author keywords

Collagenase digestion; Pig pancreatic islets

Indexed keywords

EID: 0008331370     PISSN: 0908665X     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1399-3089.1997.tb00165.x     Document Type: Article

References (18)

1. RICORDI C, FINKE EH, LACY PE. Method for the mass isolation of islets from the adult pig pancreas. Diabetes 1986:35:649.
2. MARCHETTII PA, GIANNARELLI R, et al. Isolation of the islets of Langerhans from the adult pig pancreas. Transpl Proc 1988:20:707.
3. CROWTHER NJ, GOTFREDSEN CF, MOODY AJ, et al. Porcine islet isolation, cellular composition and secondary response. Horm Metabol Res 1989:21:590.
4. RICORDI C et al. Isolation of the elusive pig islet. Surgery 1990:107:688.
5. MARCHETTI P, FINKE EH, GERASMID-VAZEAU A, et al. Automated large-scale isolation, in vitro function and xenotransplantation of porcine islets of Langerhans. Transplantation 1991:52:209.
6. CALAFIORE R, CALCINVARO F, BASTA G, et al. A method for the massive separation of purified, adult porcine islets of Langerhans. Metabolism 1990:39:175.
7. BASTA G, FALORNI A, OSTRICIOLI L, BRUNETTI P, CALAFIORE R. Method for mass retrieval, morphologic, and functional characterization of adult porcine islets of Langerhans: A potential nonhuman pancreatic tissue resource for xenotransplantation in insulin-dependent diabetes mellitus. J Invest Med 1995:43:555.
8. SUN YL, MA X, ZHOU D, VACEK I, SUN AM. Porcine pancreatic islets: Isolation, microencapsulation and xenotransplantation. Artif Organs 1992:17:727.
9. ZHOU D, YANG B, SUN Y, VACEK I, SUN AM. Effects of collagenase concentration on the purity and viability of isolated porcine pancreatic islets for xenotransplantation. Xenotransplantation 1996:3:11.
10. DONO K, GOTOH M, FUKUZAKI T, OHZATO H, KANAI T, MONDEN M, MORI T. Low-temperature collagenase digestion: An improved islet isolation method from cold preserved pancreas. Transpl Proc 1992:24:1511.
11. FAN MY, LUM X, FU X, LEVESQUE L, TAI IT, SUN AM: Reversal of diabetes in BB rats by transplantation of encapsulated rat islets. Diabetes 1990:39:519.
12. HOMMEL M, SUN AM, GOOSEN MFA. Droplet generation. Canadian Patent 458605, 1984.
13. BUHLER R, DENG R, MAGE R, BUBLOZ C, ROHNER A, MOREL P. Pig islet isolation: New aspects. Transpl Proc 1994:26:628.
14. VILLANI G, MARCHETTI P, GINNARELLI R, CRUSCHELLI L, ANDREOZZI M, COSIMI S, NAVALEZI R. Comparison of different digestion techniques and density gradient purification procedures to prepare viable pancreatic islets from market age pigs. Transpl Proc 1994:26:618.
15. HEISER A, ULRICHS K, MULLER-RUCHHOLTZ W. Influence of porcine strain, age, and pH of the isolation medium on porcine pancreatic islet isolation success. Transpl Proc 1994:26:618.
16. TAI J, TSANG A, TZE WJ. Effects of serum and medium supplements, pH, and temperature on the cultured porcine islets. Transpl Proc 1994:26:818.
17. KIRCHHOF N, HERING BJ, GLEISS V, FREDERLIN K, BRETZEL RG. Evidence for breed-depedent differences in porcine islets of Langerhans. Transpl Proc 1994:26:616.
18. VAN DEIJNEN JH, HULSTAERT CE, WOLTES GH, VAN SCHILFGAARDE R. Significance of the peri-insular extracellular matrix for islet isolation from the pancreas of rat, dog, pig and man. Cell Tissue Res 1992:267:139.