A simplified, competitive RT-PCR method for measuring rat IFN-γ mRNA expression

Journal of Immunological Methods

Volumn 195, Issue 1-2, 1996, Pages 139-148

  Sun, Bing ^a   Wells, Jana ^b   Goldmuntz, Ellen ^b   Silver, Phyllis ^a   Remmers, Elaine F ^b   Wilder, Ronald L ^b   Caspi, Rachel R ^a  

^a NATIONAL EYE INSTITUTE   (United States)

^b NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

Author keywords

DNA mimic; ELISA; Housekeeping gene; IFN mRNA; Northern blot analysis; Rat; Reverse transcriptase polymerase chain reaction

Indexed keywords

GAMMA INTERFERON; MESSENGER RNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; RAT; T LYMPHOCYTE;

ANIMALIA;

EID: 0008166218     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/0022-1759(96)00099-3     Document Type: Article

References (12)

1. Arnheim, N. and Erlich, H. (1992) Polymerase chain reaction strategy, Annu. Rev. Biochem. 61, 131.
2. Babu, J.S., Kanangat, S. and Rouse, B.T. (1993) Limitations and modifications of quantitative polymerase chain reaction. Application to measurement of multiple mRNAs present in small amounts of sample RNA. J. Immunol. Methods 165, 207.
3. Chomczynski, P. (1993) A reagent for the single-step simultaneous isolation of RNA, DNA and proteins from cell and tissue samples. Biotechniques 15, 532.
4. During, K. (1991) Ultrasensitive chemiluminescent and colorigenic detection of DNA, RNA, and proteins in plant molecular biology. Anal. Biochem. 196, 433.
5. Gause, W.C. and Adamovicz, J. (1994) The use of the PCR to quantitate gene expression. PCR Methods Appl. 3, S123.
6. Gilliland, G., Perrin, S., Blanchard, K. and Bunn, H.F. (1990) Analysis of cytokine mRNA and DNA: detection and quantitation by competitive polymerase chain reaction. Proc. Natl. Acad. Sci. USA 87, 2725.
7. Kotewicz, M.L., Sampson, C.M., D'Alessio, J.M. and Gerard, G.F. (1988) Isolation of cloned Moloney murine leukemia virus reverse transcriptase lacking ribonuclease H activity. Nucleic Acids Res. 16, 265.
8. O'Garra, A. and Vieira, P. (1992) Polymerase chain reaction for detection of cytokine gene expression. Curr. Opin. Immunol. 4, 211.
9. Sanui, H., Redmond, T.M., Kotake, S., Wiggert, B., Hu, L.H., Margalit, H., Berzofsky, J.A., Chader, G.J. and Gery, I. (1989) Identification of an immunodominant and highly immunopathogenic determinant in the retinal interphotoreceptor retinoid-binding protein (IRBP). J. Exp. Med. 169, 1947.
10. Savion, S., Oddo, S., Grover, S. and Caspi, R.R. (1994) Uveitogenic T lymphocytes in the rat: pathogenicity vs. lymphokine production, adhesion molecules and surface antigen expression. J. Neuroimmunol, 55, 35.
11. Siebert, P.D. and Larrick, J.W. (1993) PCR MIMICS: competitive DNA fragments for use as internal standards in quantitative PCR. Biotechniques 14, 244.
12. Wang, A.M., Doyle, M.V. and Mark, D.F. (1989) Quantitation of mRNA by the polymerase chain reaction. Proc. Natl. Acad. Sci. USA 86, 9717.