Isolation of protoplasts from viable egg cells of common buckwheat (Fagopyrum esculentum Moench.)

Canadian Journal of Plant Science

Volumn 79, Issue 4, 1999, Pages 593-595

  Woo, Sun Hee ^a   Adachi, Taiji ^b   Jong, Seung Keun ^c   Campbell, Clayton G ^d  

^a AGRICULTURE AND AGRI FOOD CANADA   (Canada)

^b UNIVERSITY OF MIYAZAKI   (Japan)

^c Chungbuk National University   (South Korea)

^d Kade Research Ltd   (Canada)

Author keywords

Common buckwheat; Egg cell solation; Embryo sac; Fagopyrum esculentum Moench.; Protoplast

Indexed keywords

EGG CELL; ENZYMIC TREATMENT; IN VITRO FERTILIZATION; OVULE CULTURE; PROTOPLAST; PROTOPLAST ISOLATION;

FAGOPYRUM ESCULENTUM;

EID: 0008047838     PISSN: 00084220     EISSN: None     Source Type: Journal    
DOI: 10.4141/P98-118     Document Type: Article

References (11)

1. Faure, J. E., Mogensen, H. L., Kranz, E., Digonnet, C. and Dumas, C. 1992. Ultrastructural characterization and three-dimentional reconstructional of isolated maize (Zea may L.) egg cell protoplasts. Protoplasma 171: 97-103.
2. Frearson, E. M., Power, J. B. and Cocking, E. C. 1973. The isolation, culture, and regeneration of petunia leaf protoplasts. Devel. Biol. 33:130-137.
3. Guan, L. M. and Adachi, T. 1992. Reproductive deterioration in buckwheat (Fagopyrum esculentum) under summer conditions. Plant Breed. 109:304-312.
4. Heslop-Harrison, J. and Heslop-Harrison, Y. 1970. Evaluation of pollen viability by enzymatically induced fluorescence: intracellular hydrolysis of fluorescein diacetate. Stain Technol. 45:115-120.
5. Kovacs, M., Barnabas, B. and Kranz, E. 1994. The isolation of viable egg cells of wheat (Triticum aestivum L.). Sex Plant Reprod. 7:311-312.
6. Kranz, E. and Lörz, H. 1993. In vitro fertilization with isolated, single gametes results in zygotic embryogenesis and fertile maize plants. Plant Cell 5: 739-746.
7. Kranz, E., Bautor, J. and Lörz, H. 1991. In vitro fertilization of single, isolated gametes of maize mediated by electrofusion. Sex Plant Reprod. 4: 12-16.
8. Kranz, E., von Wiegen, P. and Lörz, H. 1995. Early cytological events after induction of cell division in egg cells and zygote development following in vitro fertilization with angiosperm gametes. Plant J. 8(1): 9-23.
9. Marshall, H. G. and Pomeranz, Y. 1983. Buckwheat: Description, breeding, production and utilization. Adv. Cereal Sci. Technol. 5: 157-210.
10. Woo, S. H., Tsai, Q. S. and Adachi, T. 1995. Possibility of interspecific hybridization by embryo rescue in the genus Fagopyrum. Curr. Adv. Buckwheat Res. 6: 225-237.
11. You, R. and Jensen, W. A. 1985. Ultrastructural observations of the mature megagametophyte and the fertilization in wheat. Can. J. Bot. 63: 163-178.