Baro-immunodesorption: Development of new affinity supports suitable for semi-continuous processes. Application to the purification of recombinant Escherichia coli β-galactosidase, and comparison with a conventional purification method

High Pressure Research

Volumn 19, Issue 1-6, 2000, Pages 291-296

  Estevez Burugorri, L ^a   Lemay, P ^a  

^a UMR792 INGÉNIERIE DES SYSTÈMES BIOLOGIQUES ET DES PROCÉDÉS   (France)

Author keywords

Barodesorption; E. Coli galactosidase; High pressure; Immunoaffinity supports; Process; Purification

Indexed keywords

EID: 0008026594     PISSN: 08957959     EISSN: None     Source Type: Journal    
DOI: 10.1080/08957950008202567     Document Type: Article

References (11)

1. Bradford, M. (1976). Anal. Biochem., 72, 248.
2. Casadaban, M. J., Martinez-Arias, A., Shapira, S. K. and Chou, J., Methods in Enzymol., 100 [Academic Press Inc., New York, 1983], 293.
3. Degraeve, P. and Lemay, P. (1997). Enz. Microbial Technol., 20, 551.
4. Degraeve, P. and Lemay, P., High Pressure Research in the Bioscience and Biotechnology [Ed. Heremans, K., Leuven University Press, Leuven, 1997], p. 115.
5. Gavalda, E., Degraeve, P. and Lemay, P. (1996). Enz. Microbial Technol., 18, 10.
6. Green, D. J., Litt, G. J. and Laugharn, J. A. (1998). Clinical Chemistry, 44, 341.
7. Howlett, J. R., Ismail, A. A., Amstrong, D. W. and Wong, P. T. T. (1992). Biochim. Biophys. Acta, 1159, 227.
8. Miron, T. and Wilchek, M. (1981). J. Chromatog., 215, 55.
9. Monsan, P., Ph.D. Thesis [Univ. ToulouseIII, Toulouse, 1977], p. 46.
10. Sanchez-Puelles, J. M., Sanz, J. M., Garcia, J. L. and Garcia, E. (1992). Eur. J. Biochem., 203, 153.
11. Ternynk, T. and Avrameas, S., Techniques Immunoenzymatiques [Ed. INSERM, Paris, 1987], p. 18.