Evaluation of lacZ and gusA markers to study rhizobial colonization in rice roots

Indian Journal of Microbiology

Volumn 40, Issue 1, 2000, Pages 15-20

  Saxena, S ^a   Ladha, J K ^a   Gyaneshwar, P ^a   Reinhold Hurek, B ^b   Hernandez, R J ^a   Biswas, J C ^a  

^a INTERNATIONAL RICE RESEARCH INSTITUTE   (Philippines)

^b M P Inst Terrestrische Mikrobiol   (Germany)

Author keywords

Colonization; gusA; lacZ; Molecular markers; Rhizobium; Rice

Indexed keywords

EID: 0007982525     PISSN: 00468991     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. Christiansen-Weniger C (1996) Endophytic establishment of Azorhizobium caulinodans through auxin-induced root tumors of rice. Biol Fertil Soils 21:293-302.
2. Reddy PM, Ladha JK, So R, Hernandez R, Dazzo FB, Angeles OR, Ramos MC & de Bruijn FJ (1997) Rhizobial communication with rice roots: induction of phenotypic changes, mode of invasion and extent of colonization. Plant Soil 194:81-98.
3. Webster G, Cough C, Vasse J, Batchelor CA, O'Callaghan KJ, Kothari SL, Davey MR, Denarie J & Cocking EC (1997) Interaction of rhizobia with rice and wheat. Plant Soil 194:115-122.
4. Bilal R, Rasul G, Arshad M & Malik KA (1993) Attachment, colonization and purification of Azospirillum brasilense and Enterobaceter sp. on root surfaces of grasses. World J Microbiol Biotechnol 9:63-69.
5. Arsene F, Katupitiya S, Kennedy IR & Elmerich C (1994) Use of lacZ fusions to study the expression of nif genes of Azospirillum brasilense in association with plants. Mol Plant Microbe Interact 7:748-757.
6. Katupitiya, S, Millet J, Vesk M, Viccars L, Zeman A, Lidong Z, Elmerich C & Kennedy IR (1995) A mutant of Azospirillum brasilense SP7 impaired in flocculation with a modified colonization pattern and superior nitrogen fixation in association with wheat. Appl Environ Microbiol 61:1987-1995.
7. 2 fixation in 2,4-D treated wheat roots associated with Azospirillum lipoferum: studies of colonization using reporter genes. Soil Biol Biochem 27:447-452.
8. Hurek T, Reinhold-Hurek B, Van Montagu M & E. Kellenberger (1994) Root colonization and systemic spreading of Azoarcus sp. Strain BH72 in grasses. J Bacteriol 176:1913-1923.
9. Gage DJ, Bobo T & S. Long SR (1996) Use of green fluorescent protein to visualize the early events of symbiosis between Rhizobium meliloti and alfalfa (Medicago sativa). J Bacteriol 178:7159-7166.
10. Wilson KJ (1995) Molecular techniques for the study of rhizobial ecology in the field. Soil Biol Biochem 27:501-514.
11. Silhavy TJ & Beckwith JR (1985) Use of LacZ fusions for the study of biological problems. Microbiol Rev 49:398-418.
12. Jefferson RA (1987) Assaying chimeric genes in plants: the GUS gene fusion system. Plant Mol Biol Rep 5:387-405.
13. Chalfie M, Tu Y, Eukirchen G, Ward WW, & Prasher DC (1994) Green fluorescxent protein as a marker for gene expression. Science 263:802-805.
14. Huber D (1983) The role of cell wall hydrolases in fruit softening. Hort Rev 5:169-219.
15. Seymour GB & Gross KC (1996) Cell wall disassembly and fruit softening. Postharvest News Info 7:45N-52N.
16. Boivin C, Camet S, Malpica CA, Truchet G & Rosenberg C (1990) Rhizobim meliloti gene encoding catabolism of trigonellin are induced under symbiotic conditions. Plant Cell 2:1157-1170.
17. Terri TH, Lehvaslaiho H, Franck M, Uotila J, Hsino P, Palva ET, van Montagu M & Herrera-Estrella L (1989) Gene fusions to lacZ reveal new expression patterns of chimeric gene in transgenic plants. EMBO J 8:343-350.
18. Wilson KJ, Sessitsch A, Corbo JC, Giller KE, Akkermans ADL & Jefferson RA (1995) β-Glucuronidase (GUS) transposons for ecological and genetic studies of rhizobia and other Gram negative bacteria. Microbiology 141:1691-1705.
19. Van de Broek A, Michiels J, de Fari SM, Milcaps A & Vanderleyden J (1992) Transcription of the Azospirillum brasilense nifH gene is positively regulated by Nif A and Ntr A and is negatively controlled by the cellular nitrogen status. Mol Gen Genet 232:279-283.
20. Van de Broek A, Michiels J, Van Cool A & Vanderleyden J (1993) Spatial and temporal colonization patterns of Azospirillum brasilense on wheat root surface and expression of bacterial nifH gene during association. Mol Plant-Microbe Interact 6:592-600.
21. Christiansen-Weninger C & Venderleyden J (1994) Ammonia excreting Azospirillum sp. become intercellularly established in maize (Zea mays) para nodules. Biol Fertil Soils 17:1-8.
22. Ladha JK, deBruijn FJ & Malik KA (1997) Introduction: Assessing opportunities for nitrogen fixation in rice -A frontier project. Plant Soil 194:1-10.
23. Leong SA, Williams PH & Ditta GS (1985) Analysis of 5' regulatory region of the gene for δ-amino levulinic acid synthase of Rhizobium meliloti. Nucl Acid Res 13: 5965-5976.
24. Somasegaran P & Hoben HJ (1994) Handbook for Rhizobia. Springer-Verlag, New York, USA, pp. 450.
25. Fahraeus G (1957) The infection of clover root hairs by nodule bacteria studied by a simple glass slide techniques. J Gen Microbiol 16:374-381.
26. Stomp A (1987) Histochemical localization of β-glucuronidase. In: Gus Protocols: Using Gus Gene as Reporter Gene Expression (Gallagher SR ed), Academic Press, Inc. New York, pp. 103-113.
27. Reinhold-Hurek B & Hurek T (1998) Interactions of graminaceous plants with Azoarcus spp. and other diazotrophs: identification, localization and perspective to study their function. Crit Rev Plant Sci 176:29-54.
28. James EK & Olivares FL (1998) Infection and colonization of sugarcane and other graminaceous plants by endophytic diazotrophs. Crit Rev Plant Sci 17:77-119.
29. Reddy PM, Aggarwal RK, Ramos MC, Ladha JK, Brar DS & Kouchi H (1999) Widespread occurrence of the homologues of the early nodulin (ENOD) genes in Oryza species and related grasses. Biochem Biophys Res Commun 258:148-154.