Continuous production of fructose-syrups from inulin by immobilized inulinase from recombinant Saccharomyces cerevisiae

Biotechnology and Bioprocess Engineering

Volumn 2, Issue 2, 1997, Pages 90-93

  Kim, Byung Woo ^a   Kim, Han Woo ^a   Nam, Soo Wan ^a  

^a Dong Eui University   (South Korea)

Author keywords

Fructose syrup; Immobilization; Packed bed reactor; Recombinant inulinase

Indexed keywords

EID: 0007798944     PISSN: 12268372     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02932331     Document Type: Article

References (8)

1. Snyder, H. E. and J. Phaff (1962) The pattern of action of inulinase from Saccharomyces fragilis on inulin. J. Biol. Chem. 237: 2438-2441.
2. Bajpai, P. K. and P. Bajpai (1991) Cultivation and utilization of Jerusalem artichoke for ethanol, single cell protein, and high-fructose syrup production. Enzyme Microb. Technol. 13: 359-362.
3. Kim, C. H. and S. K. Rhee (1989) Fructose production from Jerusalem artichoke by inulinase immobilized on chitin. Biotechnol. Lett. 11: 201-206.
4. Laloux, O., J. P. Cassart, J. Delcour, J. van Beeumen, and J. Vandenhaute (1991) Cloning and sequencing of the inulinase gene of Kluyveromyces marxianus var. marxianus ATCC 12424. FEBS Lett. 289: 64-68.
5. Nam, S. W., K. Yoda, and M. Yamasaki (1993) Secretion and localization of invertase and inulinase in recombinant Saccharomyces cerevisiae. Biotehnol. Lett. 15: 1049-1054.
6. Hill, H. D. and J. G. Straka (1988) Protein determination using bicinhoninic acid in the presence of sulfhydryl reagents. Anal. Biochem. 170: 203-208.
7. Nakamura, T., Y. Ogata, A. Shitara, A. Nakamura, and K. Ohta (1995) Continuous production of fructose syrups from inulin by immobilized inulinase from Aspergilus niger mutant 817. J. Ferment. Bioeng. 80: 164-169.
8. Rouwenhorst, R. J., M. Hensing, J. Verbakel, W. A. Scheffers, and J. P. Van Dijken (1990) Structure and properties of the extracellular inulinase of Kluyveromyces marxianus CBS 6556. Appl. Environ. Microbiol. 56: 3337-3345.