Effect of the entomopathogenic fungus Beauveria bassiana on the humoral immune response of Galleria mellonella larvae (Lepidoptera: Pyralidae)

European Journal of Entomology

Volumn 94, Issue 4, 1997, Pages 461-472

  Vilcinskas, Andreas ^a   Maťha, Vladimir ^b  

^a FREIE UNIVERSITÄT BERLIN   (Germany)

^b TEVA PHARMACEUTICALS   (Israel)

Author keywords

Beauveria bassiana; Entomopathogenic fungus; Galleria mellonella; Humoral immunity; Pyralidae; Suppression

Indexed keywords

BASSIANA; BEAUVERIA; CORDYCEPS BASSIANA; FUNGI; GALLERIA MELLONELLA; HYMENOPTERA; LEPIDOPTERA; NEMATODA; PYRALIDAE;

EID: 0007555611     PISSN: 12105759     EISSN: 18028829     Source Type: Journal    
DOI: None     Document Type: Article

References (29)

1. BOMAN H.G., NILSSON-FAYE I., PAUL K. & RASMUSON J.T. 1974: Insect immunity I. Characteristics of an inducible cell-free antibacterial reaction in hemolymph of Samia cynthia pupae. Infect, Immunol. 10: 136-145.
2. BREY P.T., LEE W.-J., YAMAKAWA M., KOIZUMI Y., PERROT S., FRANCOIS M. & ASHIDA M. 1993: Role of the integument in insect immunity: epicuticular abrasion and induction of cecropin synthesis in cuticular epithelial cells. Proc. Natn. Acad. Sci. USA 194: 359-364.
3. COCIANCICH S., BULET P., HÉTRU C. & HOFFMANN J.A. 1994: The inducible antibacterial peptides of insects. Parasitol. Today 10: 132-139.
4. COLLINS M.S. & PAPPAGIANIS D. 1974: Inhibition by lysozyme of the growth of spherule phase of Coccidoides immitis in vitro. Infect. Immunol. 10: 616-623.
5. DE VERNO P.J., CHADWICK S.S., ASTON W.P. & DUNPHY G.B. 1984: The in vitro generation of an antibacterial activity from the fat body and hemolymph of non-immunized larvae of Galleria mellonella. Dev. Comp. Immunol. 8: 537-546.
6. EDLUND T., SIDEN I. & BOMAN H.G. 1976: Evidence for two immune inhibitors from Bacillus thuringiensis interfering with the humoral defense system of Saturniid pupae. Infect, Immunol. 14: 934-941.
7. FAYE I. & WYATT G.R. 1980: The synthesis of antibacterial proteins in isolated fat body from Cecropia silkmoth pupae. Experientia 36: 1325-1326.
8. FEHLBAUM P., BULET P., MICHALIT L., LAGUEUX M. & BROEKAERT W.F. 1995: Septic injury of Drosophila induces the synthesis of a potent antifungal peptide with sequence homology to plant antifungal peptides. J. Biol. Chem. 269: 33159-33163.
9. GILLESPIE A.T. & CLAYDON N.C. 1989: The use of entomogenous fungi for pest control and the role of toxins in pathogenesis. Pestic. Sci. 27: 203-215.
10. GÖTZ P. 1991: Invertebrate immune response to fungal cell wall components. In Latgé L.-P. & Boucias D. (eds): Fungal Cell and Immune Response. NATO ASI Series, Series H: Cell Biology, Vol. 53. Springer, Berlin, pp. 317-330.
11. GÖTZ P. & TRENCZEK T. 1991 : Antibacterial proteins in insects other than Lepidoptera and Diptera and in some other invertebrates. In Gupta A.P. (ed.): Immunology of Insects and Other Arthropods. CRC Press, Boca Raton, USA, pp. 323-346.
12. GÖTZ P., BOMAN A. & BOMAN H.G. 1981: Interactions between insect immunity and an insect-pathogenic nematode with symbiontic bacteria. Proc. R. Soc. London 212: 333-350.
13. HOFFMANN D., HULTMARK D. & BOMAN H. 1981: Insect immunity: Galleria mellonella and other Lepidoptera have cecropia-P9-like factors active against gram negative bacteria, Insect Biochem. 11: 537-548.
14. HOFFMANN J.A. 1995: Innate immunity of insects. Curr. Opin. Immunol. 7: 4-10.
15. IUIMA R., KURATA S. & NATORI S. 1993: Purification, characterization and cDNA cloning of an antifungal protein from the hemolymph of Sarcophaga peregrina (Flesh Fly) Larvae. J. Biol. Chem. 268: 12055-12061.
16. JAYNES J. 1989: Peptides to the rescue. New Sci. 11: 42-44.
17. KHACHATOURIANS G.G. 1996: Biochemistry and molecular biology of entomopathogenic fungi. In Howard D.H. & Miller J.D. (eds): The Mycota VI, Human and Animal Relationships. Springer, Berlin-Heidelberg, pp. 331-363.
18. LAEMMLI U.K. 1970: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature (London) 227: 680-685.
19. LEMAITRK B., KROMER-METZGER E., MICHAUT L., NICOLAS E., MEISTER M., GEORGEL P., REICHART J.-M. & HOFFMAN J.A. 1995: A recessive mutation, immune deficiency (imd), defines two distinct control pathways in the Drosophila host defense. Proc. Natn. Acad. Sci. USA 92: 9465-9469.
20. MAŤHA V., GRUBHOFFER L. WEYDA F. & HERMANNOVÁ L. 1990: Detection of ß-1,3-glucan-specific lectin on the surface of plasmatocytes, immunocompetent cells of great wax moth, Galleria mellonella L. Cytobioa 64: 35-42.
21. MOHRIG W. & MESSNER B. 1968: Immunreaktionen bei Insekten. I. Lysozym als grundlegender antibakterieller Faktor im humoralen Abwehrsystem der Insekten. Biol. Zentbl. 4: 439-469.
22. RATCMFFE N.A. 1993: Cellular defense responses of insects: unresolved problems. In Beckage N.E., Thompson S.N. & Federici D.A. (eds): Parasites and Pathogens of Insects. Vol. 1: Parasites. Academic Press, London, pp. 267-304.
23. TANADA Y. & KAYA H.K. 1993: Insect Pathology. Academic Press, London, pp. 319-385.
24. VEY A. & GÖTZ P. 1986: Antifungal cellular defence mechanisms in insects. In Gupta A.P. (ed.): Hemocytic and Humoral Immunity in Arthropods. John Wiley, New York, pp. 89-115.
25. VILCINSKAS A. 1994: Biochemische und immunologische Untersuchungen zur humoralen Abwehr von Pilzinfektionen bei Insekten am Beispiel der großen Wachsmotte Galleria mellonella (Lepidoptera). PhD thesis, Free University of Berlin, Berlin, 230 pp.
26. VILCINSKAS A. & MAŤHA V. 1997: Antimycotic activity of lysozyme and its contribution to antifungal humoral defence reactions in Galleria mellonella. Anim. Biol. 6: 13-23.
27. VILCINSKAS A., MAŤHA V. & GÖTZ P. 1997a: Inhibition of phagocytic activity of plasmatocytes from Galleria mellonella by entomogenous fungi and their secondary metabolites. J. Insect Physiol. 43: 475-483.
28. VILCINSKAS A., MAŤHA V. & GÖTZ P. 1997b: Effects of the entomopathogenic fungus Metarhizium anisopliae and its secondary metabolites on morphology and cytoskeleton of plasmatocytes isolated from the greater wax moth, Galleria mellonella. J. Insect Physiol. (in press).
29. VINSON S.B. 1993: Suppression of the insect immune system by parasitic Hymenoptera. In Pathak J.P.N. (ed.): Insect Immunity. Kluwer Academic Publishers, Dordrecht, Boston, London, pp. 171-187.