A simple method to assess the relative amount of collagen deposition in wounded fetal mouse limbs

Wound Repair and Regeneration

Volumn 4, Issue 4, 1996, Pages 489-495

  Houghton, Pamela E ^a,b   Keeper, Kerry A ^a   Diegelmann, Robertf ^c   Krummel, Thomas M ^a  

^a PENN STATE COLLEGE OF MEDICINE   (United States)

^b UNIVERSITY OF WESTERN ONTARIO   (Canada)

^c VIRGINIA COMMONWEALTH UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0007378772     PISSN: 10671927     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1524-475X.1996.40414.x     Document Type: Article

References (13)

1. Mast BA, Diegelmann RF, Krummel TM, Cohen IK Scarless wound healing in the mammalian fetus. Surg Gynecol Obs 1992;174:441-51.
2. Whitby DJ, Ferguson MW. The extracelluar matrix of lip wounds in fetal neonatal and adult mice. Development 1991;112:651-68.
3. Bleacher JC, Adolph VR, Dillon PW, Krummel TM. Isolated fetal mouse limbs: gestational effects on tissue repair in an unperfused system. J Pediatr Surg 1993;28:1312-6.
4. Houghton PE, Reefer KA, Krummel TM. The role of transforming growth factor-β in the conversion from "scarless" healing to healing with scar formation. Wound Rep Reg 1995;3:229-36.
5. Peterkofsky B, Diegelmann RF. Use of a mixture of proteinase-free collagenases for the specific assay of radioactive collagen in the presence of other proteins. Biochem 1971;10:988-94.
6. Favaloro EJ, Grispo L, Exner T, Koutts J. Development of a simple collagen based ELISA assay aids in the diagnosis of, and permits sensitive discrimination between Type I and Type II, von Willebrand's disease. Blood Coagul Fibrinolysis 1991;2:285-91.
7. Clore JN, Cohen IK, Diegelmann RF. Quantitation of collagen types I and II during wound healing in rat skin, Proc Soc Biol Med 1979;161:337-40.
8. Bardsley WG, Sattar A, Armstrong JR, Shah M, Brosnan P, Ferguson MWJ. Quantitative analysis of wound healing. Wound Rep Reg 1995;3:426-41.
9. Pierce GF, Brown D, Mustoe TA. Quantitative analysis of inflammatory cell influx, procollagen type I synthesis, and collagen cross linking in incisional wounds: influence of PDGF-BB and TGF-B1 therapy. J Lab Clin Med 1991;117:373-81.
10. Pierce GF, Tarpley JE, Yanagihara D, Mustoe TA, Fox GM, Thomasen A. Platelet-derived growth factor (BB-homodimer), transforming growth factor-B1, and basic fibroblast growth factor in dermal wound healing. Am J Pathol 1992;140:1375-86.
11. Gorodetsky R, McBride WH, Withers HR, Miller GG. Effect of fibroblast implants on wound healing of irradiated skin: assay of wound strength and quantitative immunohistology of collagen. Radiat Res 1991;125:181-5.
12. Hershman KM, Fleming WW, Taylor DA. A quantitative method for assessing protein abundance using enhanced chemiluminescence. Biotechniques 1993;15:790, 792, 794 passim.
13. Lopez de Leon A, Rojkind M. A simple micromethod for collagen and total protein determination in formalin-fixed paraffin-embedded sections. J Histo Cytochem 1985;33:737-43.