Antibodies to N-terminal peptides of low Mr subunits of wheat glutenin. II. Detection of subunits encoded by different loci

Journal of Cereal Science

Volumn 30, Issue 3, 1999, Pages 283-301

  Sissons, M J ^a   Hac, L ^a   Skerritt, J H ^a  

^a CSIRO   (Australia)

Author keywords

Allelic variation; Antibodies; Biotypes; Chromosomal control; Glutenin subunits; Immunoblotting; N terminal sequence

Indexed keywords

SECALE CEREALE; TRITICUM AESTIVUM;

EID: 0007064132     PISSN: 07335210     EISSN: None     Source Type: Journal    
DOI: 10.1006/jcrs.1999.0276     Document Type: Article

References (22)

1. r subunits of wheat glutenin. I. Characterisation of the antibody response. Journal of Cereal Science 30 (1999) 267-281.
2. Gupta, R.B. and Shepherd, K.W. Two-step one-dimensional SDS-PAGE analysis of LMW subunits of glutenin. I. Variation and genetic control of the subunits in hexaploid wheats. Theoretical and Applied Genetics 80 (1990) 65-74.
3. Sissons, M.J., Bekes, F. and Skerritt, J.H. Isolation and functionality of low molecular weight glutenin subunits. Cereal Chemistry 75 (1998) 30-36.
4. Shewry, P.R., Halford, N.G. and Tatham, A.S. The high molecular weight subunits of wheat glutenin. Journal of Cereal Science 15 (1992) 105-120.
5. Gupta, R.B. and Shepherd, K.W. Production of multiple wheat-rye 1RS translocation stocks and genetic analysis of LMW subunits of glutenin and gliadin in wheats using these stocks. Theoretical and Applied Genetics 85 (1993) 719-728.
6. Melas, V., Morel, M.H., Autran, J.C. and Feillet, P. Simple and rapid method for purifying low molecular weight subunits of glutenin from wheat. Cereal Chemistry 71 (1994) 234-241.
7. Huebner, F.R. and Bietz, J.A. Improved chromatographic separation of ethanol soluble wheat proteins. Cereal Chemistry 70 (1993) 506-511.
8. Partridge, M.A.K., Sissons, M.J. and Skerritt, J.H. Development of allele specific probes for loci encoding gamma and omega gliadins and LMW-GS. In 'Proceedings of the 8th Wheat Breeding Society of Australia' (R. Richards, C.W. Wrigley, H.M. Rawson, G.J. Rebetzke, J.L. Davidson and R.I.S. Brettell, eds), Canberra (1996) pp P59-P63.
9. Skerritt, J.H. and Underwood, P.A. Specificity characteristics of monoclonal antibodies to wheat grain storage proteins. Biochimica et Biophysica Acta 874 (1986) 245-254.
10. Bradford, M.M. A rapid and sensitive method for the quantification of microgram quantities of protein utilising the principle of protein-dye binding. Analytical Biochemistry 72 (1976) 248-254.
11. Andrews, J. and Skerritt, J.H. Wheat dough extensibility screening using a two-site enzymelinked immunosorbent assay (ELISA) with antibodies to low molecular weight glutenin subunits. Cereal Chemistry 73 (1996) 650-657.
12. Kaufmann, S.H. and Shaper, J.H. Erasable Western blots. In 'Method in Molecular Biology, Vol. 10: Immunochemical protocols' (M. Manson, ed.) Humana Press Inc. New York, U.S.A. (1992) 235-246.
13. Skerritt, J.H., Martinuzzi, O. and Metakovsky, E.V. Chromosomal control of wheat gliadin protein epitopes: analysis with specific monoclonal antibodies. Theoretical and Applied Genetics 82 (1991) 44-53.
14. Autran, J.C., Lew, E.J.-L., Nimmo, C.C. and Kasarda, D.D. N-terminal amino acid sequences of prolamins from wheat and related species. Nature 282 (1984) 527-529.
15. D'Ovidio, R., Simeone, M., Masci, S., Porceddu, E. and Kasarda, D.D. Nucleotide sequence of a γ-type glutenin gene from a durum wheat: Correlation with a γ-type glutenin subunit from the same biotype. Cereal Chemistry 72 (1995) 443-449.
16. Masci, S., Lafiandra, D., D'Ovidio, R. and Kasarda, D.D. Identification of cysteine residues in a LMW-glutenin subunit that is associated with good quality characteristics. In 'Gluten Proteins 1996, Proceedings of the 6th International Gluten Workshop' (C .W. Wrigley, ed.) Royal Australian Chemical Institute, Melbourne, Australia. (1996) pp 90-93.
17. Okita, T.W., Cheesebrough, V. and Reeves, C.D. Evolution and heterogeneity of the α/β-type and γ-type gliadin DNA sequences. Journal of Biological Chemistry 260 (1985) 8203-8213.
18. Lew, EJ.L., Kuzmicky, D.D. and Kasarda, D.D. Characterisation of low molecular weight glutenin subunits by reversed-phase high-performance liquid chromatography, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and N-terminal amino acid sequencing. Cereal Chemistry 69 (1992) 508-515.
19. r subunits of wheat glutenin and their use in the detection of allelic variants at Glu-3 loci. Journal of Cereal Science 22 (1995) 222-235.
20. Skerritt, J.H. and Robson, L.G. Wheat low molecular weight glutenin subunits-structural relationships to other gluten proteins analyzed using specific antibodies. Cereal Chemistry 67 (1990) 250-257.
21. Singh, N.K., Shepherd, K.W. and Cornish, G.B. A simplified SDS-PAGE procedure for separating LMW subunits of glutenin. Journal of Cereal Science 14 (1991) 203-208.
22. Masci, S., Lew, E.J.-L., Lafiandra, D., Porceddu, E. and Kasarda, D.D. Characterization of low molecular weight glutenin subunits in durum wheat by reversed-phase high performance liquid chromatography and N-terminal sequencing. Cereal Chemistry 72 (1995) 100-104.