Sense and antisense coat protein gene constructs confer high levels of resistance to tomato ringspot nepovirus in transgenic Nicotiana species

Phytopathology

Volumn 86, Issue 4, 1996, Pages 417-424

  Yepes, Luz Marcela ^a   Fuchs, Marc ^a   Slightom, Jerry L ^b   Gonsalves, Dennis ^a  

^a Department of Obstetrics Gynecology   (United States)

^b PFIZER INC   (United States)

Author keywords

Engineered protection; Pathogen derived resistance

Indexed keywords

LYCOPERSICON ESCULENTUM; NEPOVIRUS; NICOTIANA; NICOTIANA BENTHAMIANA; NICOTIANA TABACUM; PRUNUS PERSICA; TABACUM; TOMATO RINGSPOT VIRUS;

EID: 0006525849     PISSN: 0031949X     EISSN: None     Source Type: Journal    
DOI: 10.1094/Phyto-86-417     Document Type: Article

References (49)

1. An, G., Ebert, P. R., Mitra, A., and Ha, S. B. 1988. Binary vectors. Pages A3:1-19 in: Plant Molecular Biology Manual. S. B. Gelvin and R. A. Schilperoort, eds. Kluwer Academic Publishers, Dordrecht, Netherlands.
2. Bacher, J. W., Warkentin, D., Ramsdell, D., and Hancock, J. F. 1994. Sequence analysis of the 3′ termini of RNA 1 and RNA 2 of blueberry leaf mottle virus. J. Gen. Virol. 75:2133-2138.
3. Bardonnet, N., Hans, F., Serghini, M. A., and Pinck, L. 1994. Protection against virus infection in tobacco plants expressing the coat protein of grapevine fanleaf nepovirus. Plant Cell Rep. 13:357-360.
4. Bertioli, D. J., Cooper, J. I., Edwards, M. L., and Hawes, W. S. 1992. Arabis mosaic nepovirus coat protein in transgenic tobacco lessens disease severity and virus replication. Ann. Appl. Biol. 120:47-54.
5. Bertioli, D. J., Harris, R. D., Edwards, M. L., Cooper, J. I., and Hawes, W. S. 1991. Transgenic plants and insect and cells expressing the coat protein of arabis mosaic virus produce empty virus-like particles. J. Gen. Virol. 72:1801-1809.
6. Bitterlin, M. W., and Gonsalves, D. 1988. Serological grouping of tomato ringspot virus isolates: Implications for diagnosis and cross-protection. Phytopathology 78:278-285.
7. Blok, V. C., Wardell, J., Jolly, C. A., Manoukian, A., Robinson, D. J., Edwards, M. L., and Mayo, M. A. 1992. The nucleotide sequence of RNA 2 of raspberry ringspot nepovirus. J. Gen. Virol. 73:2189-2194.
8. Blok, V. C., Wardell, J., Jolly, C. A., Manoukian, A., Robinson, D. J., Edwards, M. L., and Mayo, M. A. 1994. Sequence analysis of the 3′ termini of RNA 1 and RNA 2 of blueberry mottle virus. Virus Res. 33:145-156.
9. Brault, V., Candresse, T., Le Gall, O., Delbos, R. P., Lanneau, M., and Dunez, J. 1993. Genetically engineered resistance against grapevine chrome mosaic nepovirus. Plant Mol. Biol. 21:89-97.
10. Brault, V., Hibrand, L., Candresse, T., Le Gall, O., and Dunez, J. 1989. Nucleotide sequence and genetic organization of Hungarian grapevine chrome mosaic nepovirus RNA 2. Nucleic Acids Res. 17:7809-7819.
11. Brown, D. J. F., Halbrendt, J. M., Jones, A. T., Vrain, T. C., and Robbins, R. T. 1994. Transmission of three North American nepoviruses by populations of four distinct species of the Xiphinema americanum group. Phytopathology 84:646-649.
12. Buckley, B., Silva, S., and Singh, S. 1993. Nucleotide sequence and in vitro expression of the capsid protein gene of tobacco ringspot virus. Virus Res. 30:335-349.
13. Demangeat, G., Greif, C., Hemmer, O., and Fritsch, C. 1990. Analysis of the in vitro cleavage products of the tomato black ring virus RNA 1-encoded 250 K polyprotein. J. Gen. Virol. 72:247-252.
14. Dougherty, W. G., Lindbo, J. A., Smith, H. A., Parks, T. D., Swaney, S., and Proebsting, W. M. 1994. RNA-mediated virus resistance in transgenic plants: Exploitation of a cellular pathway possibly involved in RNA degradation. Mol. Plant-Microbe Interact. 7:544-552.
15. Everett, K. R., Milne, K. S., and Forster, R. L. S. 1994. Nucleotide sequence of the coat protein genes of strawberry latent ringspot virus: Lack of homology to the nepoviruses and comoviruses. J. Gen. Virol. 75:1821-1825.
16. Feinberg, A. P., and Vogelstein, B. 1984. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 137:266-267.
17. Fitchen, J. H., and Beachy, R. N. 1993. Genetically engineered protection against viruses in transgenic plants. Annu. Rev. Microbiol. 47:739-763.
18. Goldbach, R. 1987. Genome similarities between plant and animal RNA viruses. Microbiol. Sci. 4:197-202.
19. Gonsalves, D., and Slightom, J. L. 1993. Coat protein-mediated protection: Analysis of transgenic plants for resistance in a variety of crops. Semin. Virol. 4:397-405.
20. Grumet, R. 1994. Development of virus resistant plants via genetic engineering. Plant Breed. Rev. 12:47-49.
21. Gubler, U., and Hoffman, B. J. 1983. A simple and very efficient method for generating cDNA libraries. Gene 25:263-269.
22. Hans, F., and Sanfaçon, H. 1995. Tomato ringspot nepovirus protease: Characterization and cleavage site specificity. J. Gen. Virol. 76:917-927.
23. Harrison, B. D., and Murrant, A. F. 1977. Nepovirus group. No. 185 in: Descriptions of Plant Viruses. Commonw. Mycol. Inst./Assoc. Appl. Biol., Kew, England.
24. Hellen, C. U. T., Kräusslich, H., and Wimmer, E. 1989. Proteolytic processing of polyproteins in the replication of RNA viruses. Biochemistry 28:9881-9890.
25. Hepburn, A. G., White, J., Pearson, L., Maunders, M. J., Clarke, L. E., Prescott, A. G., and Blundy, K. S. 1985. The use of pNJ5000 as an intermediate vector for the genetic manipulation of Agrobacterium Ti plasmids. J. Gen. Microbiol. 131:2961-2969.
26. Hood, E. E., Helmer, G. L., Fraley, R. T., and Chilton, M. D. 1986. The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of the T-DNA. J. Bacteriol. 168:1291-1301.
27. Horsch, R. B., Fry, J., Hoffmann, N., Neidermeyer, J., Rogers, S. G., and Fraley, R. T. 1993. Leaf disc transformation. Pages A5:1-9 in: Plant Molecular Biology Manual. S. B. Gelvin, R. A. Schilperoort, and D. P. S. Verma, eds. Kluwer Academic Publishers, Dordrecht, Netherlands.
28. Jefferson, R. A. 1987. Assaying chimeric genes in plants: The GUS gene fusion system. Plant Mol. Biol. Rep. 5:387-405.
29. Kreiah, S., Strunk, G., and Cooper, J. I. 1994. Sequence analysis and location of capsid proteins within RNA 2 of strawberry latent ringspot virus. J. Gen. Virol. 75:2527-2532.
30. Lindbo, J. A., and Dougherty, W. G. 1992. Pathogen-derived resistance to a potyvirus: Immune and resistant phenotypes in transgenic tobacco expressing altered forms of a potyvirus coat protein nucleotide sequence. Mol. Plant-Microbe Interact. 5:144-153.
31. Lindbo, J. A., Silva-Rosales, L., Proebsting, W. M., and Dougherty, W. G. 1993. Induction of a highly specific antiviral state in transgenic plants: Implication for regulation of gene expression and virus resistance. Plant Cell 5:1749-1759.
32. Lutcke, H. A., Chow, K. C., Mickel, F. S., Moss, K. A., Kern, H. F., and Scheele, G. A. 1987. Selection of the AUG initiation codon differs in plants and animals. EMBO (Eur. Mol. Biol. Organ.) J. 6:43-48.
33. Meyer, M., Hemmer, O., Mayo, M. A., and Fritsch, C. 1986. The nucleotide sequence of tomato black ring virus RNA-2. J. Gen. Virol. 67:1257-1271.
34. Murray, M. G., and Thompson, W. F. 1980. Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res. 8:4321-4325.
35. Pinck, L., Fuchs, M., Pinck, M., Ravelonandro, M., and Walter, B. 1988. A satellite RNA in grapevine fanleaf virus strain F13. J. Gen. Virol. 69:233-239.
36. Quemada, H. D., Gonsalves, D., and Slightom, J. L. 1991. Expression of coat protein gene from cucumber mosaic virus strain C in tobacco: Protection against infection by CMV strains transmitted mechanically or by aphids. Phytopathology 81:794-802.
37. Rott, M. E., Gilchrist, A., Lee, L., and Rochon, D. M. 1995. Nucleotide sequence of tomato ringspot virus RNA 1. J. Gen. Virol. 76:465-473.
38. Rott, M. E., Tremaine, J. H., and Rochon, D. M. 1991. Nucleotide sequence of tomato ringspot virus RNA-2. J. Gen. Virol. 72:1505-1514.
39. Rott, M. E., Tremaine, J. H., and Rochon, D. M. 1991. Comparison of the 5′ and 3′ end termini of tomato ringspot virus RNA 1 and RNA 2: Evidence for RNA recombination. Virology 185:468-472.
40. Sanfaçon, H. 1995. Nepoviruses. Pages 129-141 in: Pathogenesis and Host Specificity in Plant Diseases. Vol. III. Viruses & Viroids. R. P. Singh, U. S. Singh, and K. Kohmoto, eds. Elsevier Science Ltd., Oxford.
41. Sanford, J. C., and Johnston, S. A. 1985. The concept of parasite-derived resistance-deriving resistance genes from the parasite's own genome. J. Theor. Biol. 113:395-405.
42. Sanger, F., Nicklen, S., and Coulson, A. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. U.S.A. 74:5463-5467.
43. Schneider, I. R., White, R. M., and Civerolo, E. L. 1974. Two nucleic acid-containing components of tomato ringspot virus. Virology 57:139-146.
44. Scott, N. W., Cooper, J. I., and Edwards, M. L. 1993. The identification, cloning, and sequence analysis of the coat protein region of a birch isolate (I2) of cherry leaf roll nepovirus. Arch. Virol. 131:209-215.
45. Scott, N. W., Cooper, J. I., Liu, Y. Y., and Helen, C. U. T. 1992. A 1.5 kb sequence homology in 3′-terminal regions of RNA-1 and RNA-2 of a birch isolate of cherry leaf roll nepovirus is also present, in part, in a rhubarb isolate. J. Gen. Virol. 73:481-485.
46. Serghini, M. A., Fuchs, M., Pink, M., Reinbolt, J., Walter, B., and Pink, L. 1990. RNA 2 of grapevine fanleaf virus: Sequence analysis and coat protein cistron location. J. Gen. Virol. 71:1433-1441.
47. Slightom, J. L. 1991. Custom polymerase-chain-reaction engineering of a plant expression vector. Gene 100:251-255.
48. Smith, H. A., Swaney, S. L., Parks, T. D., Wernsman, E. A., and Dougherty, W. G. 1994. Transgenic plant virus resistance mediated by untranslatable sense RNAs: Expression, regulation, and fate of nonessential RNAs. Plant Cell 6:1441-1453.
49. Stace-Smith, R. 1984. Tomato ringspot virus. No. 290 in: Descriptions of Plant Viruses. Commonw. Mycol. Inst./Assoc. Appl. Biol., Kew, England.