Renaturation, purification and characterization of recombinant Fab-fragments produced in Escherichia coli

Nature Biotechnology

Volumn 9, Issue 2, 1991, Pages 157-162

  Buchner, Johannes ^a   Rudolph, Rainer ^b  

^a UNIVERSITY OF REGENSBURG   (Germany)

^b F HOFFMANN LA ROCHE LTD   (Switzerland)

Author keywords

[No Author keywords available]

Indexed keywords

IMMUNOGLOBULIN F(AB) FRAGMENT; RECOMBINANT PROTEIN;

ARTICLE; ESCHERICHIA COLI; HUMAN; HUMAN TISSUE; NONHUMAN; PRIORITY JOURNAL;

ANIMAL; CHROMATOGRAPHY, GEL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; IMMUNOGLOBULINS, FAB; INCLUSION BODIES; PROTEIN CONFORMATION; RECOMBINANT PROTEINS; SUPPORT, NON-U.S. GOV'T;

ESCHERICHIA COLI; MURINAE;

EID: 0006454079     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/nbt0291-157     Document Type: Article

References (14)

1. Marston, F. A. O. 1986. The purification of eukaryotic polypeptides synthesized in Escherichia coli. Biochem. J. 240:1-12.
2. Boss, M. A., Renten, J. H., Woo, C. R., and Emtage, J. S. 1984. Assembly for functional antibodies from immunoglobulin heavy and light chains synthesised in E. coli. Nucleic Acids Res. 12:3791-3806.
3. Cabilly, S., Riggs, A. D., Pande, H., Shively, J. E., Holmes, W. E., Rey, M., Perry, L. J., Wetzel, R., and Heyneker, H. L. 1984. Generation of antibody activity from immunoglobulin chains produced in Esherichia coli. Proc. Natl. Acad. Sci. U.S.A. 81:3273-3277.
4. Plückthun, A., Glockshuber, R., Pfitzinger I., Skerra, A., and Stadlmüller, J. 1987. Engineering of antibodies with a known three-dimensional structure. Cold Spring Harbor Symp. Quant. Biol. 52:105-112.
5. Bird, R. E., Hardman, K. D., Jacobson, J. W., Johnson, S., Kaufman, B. M., Lee, S.-M., Lee, T., Pope, S. H., Riordan, G., and Whitlow, M. 1988. Single-chain antigen-binding proteins. Science 242:423-426.
6. Field, H., Yarranton, G. T., and Rees, A. R. 1988. A functional recombinant immunoglobulin variable domain from polypeptides produced in Escherichia coli, p. 29-34. In: Ginsberg, H., Brown, F., Lerner, R. A., and Chanock, R. M. (Eds.). Vaccines 88, Cold Spring Harbor, NY.
7. Huston, J. S., Levinson, D., Mudget-Hunter, M., Tai, M.-S., Novotny, J., Margolies, M. N., Ridge, R. J., Bruccoleri, R. E., Haber, E., Crea, R., and Oppermann, H. 1988. Protein engineering of antibody binding sites: Recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 85:5879-5883.
8. Condra, J. H., Sardana, V. V., Tomassini, J. E., Schlabach, A. J., Davies, M.-E., Lineberger, D. W., Graham, D. J., Gotlib, L., and Colonno, R. J. 1990. Bacterial expression of antibody fragments that block human rhinovirus infection in cultured cells. J. Biol. Chem. 265:2292-2295.
9. Buckel, P., Hübner-Parajsz, C., Mattes, R., Lenz, H., Haug, H., and Beaucamp, K. 1987. Cloning and nucleotide sequence of heavy- and light-chain cDNAs from a creatine-kinase-specific monoclonal antibody. Gene 51:13-19.
10. Jaenicke, R. 1987. Folding and association of proteins. Prog. Biophys. Mol. Biol. 49:117-237.
11. Pelham, H. R. B. 1989. Control of protein exit from the endoplasmatic reticulum. Ann. Rev. Cell Biol. 5:1-23.
12. Jaenicke, R. and Rudolph, R. 1989. Folding proteins, p. 191-223. In: Protein Structure, a Practical Approach. Creighton, T. E. (Ed.). IRL Press, Oxford.
13. Rudolph, R. 1990. Renaturation of recombinant, disulfide-bonded proteins from "inclusion bodies", p. 149-171. In: Modern Methods in Protein and Nucleic Acid Analysis. Tschesche, H. (Ed.) Walter de Gruyter, Berlin, New York.
14. Buchner, J., Jaenicke, R., and Rudolph, R. 1990. In preparation.