Efficient solid-phase synthesis of a large peptide by a single coupling protocol with a single HPLC purification step

Letters in Peptide Science

Volumn 4, Issue 3, 1997, Pages 141-146

  Nokihara, Kiyoshi ^a   Nagawa, Yoshinobu ^b   Hong, Seon Pyo ^b   Nakanishi, Hiroshi ^b  

^a Shimadzu Scientific Research Inc   (Japan)

^b NATL INST BIOSCI HUM TECHNOL   (Japan)

Author keywords

Edman sequencing; Enzyme mapping; Fmoc strategy; HATU; MALDI TOF; Solid phase synthesis

Indexed keywords

EID: 0005821184     PISSN: 09295666     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02443526     Document Type: Article

References (20)

1. For a review see: Nokihara, K., J. Syn. Org. Chem., 52 (1994) 347.
2. Small proteins consisting of 88 and 126 amino acids have been assembled and physicochemically, immunochemically and biologically characterized for comparison with natural materials. a. Nokihara, K. and Semba, T., J. Am. Chem. Soc., 110 (1988) 7847. b. Nokihara, K., Peptides, 11 (1990) 185.
3. Small proteins consisting of 88 and 126 amino acids have been assembled and physicochemically, immunochemically and biologically characterized for comparison with natural materials. a. Nokihara, K. and Semba, T., J. Am. Chem. Soc., 110 (1988) 7847. b. Nokihara, K., Peptides, 11 (1990) 185.
4. a. Nokihara, K., Yamamoto, R., Hazama, M., Wakizawa, O. and Nakamura, S., In Epton, R. (Ed.) Innovation and Perspectives in Solid Phase Synthesis 1992, Intercept, Andover, U.K., 1992, pp. 445-448.
5. b. US patent No. 5 288 464, 1994.
6. c. US patent No. 5344613, 1994.
7. d. US patent No. 5 395 594, 1995.
8. e. Takahashi, K., Knight, M. and Nokihara, K., Biomed. Prod., 10 (1995) 38.
9. a. Ando, E. and Nokihara, K., Shimadzu Rev., 53 (1996) 207.
10. b. For a review see: Wray, V., Brankenfeldt, W., Nokihara, K. and Naruse, S., In Ramage, R. (Ed.) Peptides 1996, Mayflower Scientific, Kingswinford, U.K., in press.
11. Ohme-Takagi, M. and Shinshi, H., J. Plant Cell., 7 (1995) 173.
12. Carpino, L.A., J. Am. Chem. Soc., 115 (1993) 4397.
13. Carpino, L.A., El-Faham, A., Minor, C.A. and Albericio, F., J. Chem. Soc., Chem. Commun., (1994) 201.
14. Coste, J., Le-Nguyen, D. and Castro, B., Tetrahedron Lett., 31 (1990) 205.
15. Rapp, W. and Nokihara, K., In Giralt, E. and Andreu, D. (Eds.) Peptides 1990 (Proceedings of the 21st European Peptide Symposium), ESCOM, Leiden, The Netherlands, 1991, pp. 194-195.
16. Carpino, L.A. and El-Faham, A., J. Org. Chem., 60 (1995) 3561.
17. a. Schnölzer, M. and Kent, S.B.H., Science, 256 (1992) 221.
18. b. Dawson, P.E., Muir, T.W., Clark-Lewis, I. and Kent, S.B.H., Science, 266 (1994) 776.
19. c. Rose, K., J. Am. Chem. Soc., 116 (1994) 30.
20. d. Shao, J. and Tam. J.P., J. Am. Chem. Soc., 117 (1995) 3894.