Bioenergetic and structural consequences of allotopic expression of subunit 8 of yeast mitochondrial ATP synthase. The hydrophobic character of residues 23 and 24 is essential for maximal activity and structural stability of the enzyme complex

European Journal of Biochemistry

Volumn 261, Issue 2, 1999, Pages 444-451

  Roucou, Xavier ^b   Artika, I Made ^b   Devenish, Rodney J ^b   Nagley, Phillip ^a  

^a MONASH UNIVERSITY   (Australia)

^b NONE

Author keywords

Allotopic expression; ATP synthase; Mitochondria; Oxidative phosphorylation; Yeast

Indexed keywords

ASPARTIC ACID; LEUCINE; MITOCHONDRIAL ENZYME; OLIGOMYCIN; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATASE;

AMINO ACID SUBSTITUTION; ARTICLE; BIOENERGY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ASSAY; ENZYME POLYMORPHISM; ENZYME REPRESSION; ENZYME STABILITY; ENZYME STRUCTURE; ENZYME SUBUNIT; FUNGUS GROWTH; HYDROPHOBICITY; NONHUMAN; OXIDATIVE PHOSPHORYLATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTON TRANSPORT; SACCHAROMYCES CEREVISIAE; STRUCTURE ACTIVITY RELATION;

ADENOSINE TRIPHOSPHATE; ENZYME STABILITY; FLUORESCENCE; GENE EXPRESSION REGULATION, ENZYMOLOGIC; GENE EXPRESSION REGULATION, FUNGAL; KINETICS; MITOCHONDRIA; OLIGOMYCINS; OXIDATIVE PHOSPHORYLATION; PHENOTYPE; PROTON-TRANSLOCATING ATPASES; RHODAMINE 123; SACCHAROMYCES CEREVISIAE;

FUNGI; SACCHAROMYCES CEREVISIAE;

EID: 0005776467     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1046/j.1432-1327.1999.00289.x     Document Type: Article

References (40)

1. 1. Amzel, L.M. & Pedersen, P.L. (1983) Proton ATPases: structure and mechanism. Annu. Rev. Biochem. 52, 801-824.
2. 1-ATPase from bovine heart mitochondria. Nature 370, 621-628.
3. 1-ATPase. Nature 386, 299-302.
4. 1-ATPase. J. Biol. Chem. 273, 19375-19377.
5. 1-ATPase: a rotary motor made of a single molecule. Cell 93, 21-24.
6. 6. Senior, A.E. (1988) ATP synthesis by oxidative phosphorylation. Physiol. Rev. 68, 177-231.
7. +-ATPase): results by combined biochemical and molecular biological approaches. Annu. Rev. Biochem. 58, 111-136.
8. 0-ATPase from bovine heart mitochondria. Biochemistry 30, 5369-5378.
9. 1-ATPase. Biochemistry 33, 7971-7978.
10. 10. Arselin, G., Vaillier, J., Graves, P.-V. & Velours, J. (1996) ATP synthase of yeast mitochondria; isolation of the subunit h and disruption of the ATP14 gene. J. Biol. Chem. 271, 20284-20290.
11. 11. Spannagel, C., Vaillier, J., Arselin, G., Graves, P.-V. & Velours, J. (1997) The subunit f of mitochondrial yeast ATP synthase. Characterization of the protein and disruption of the structural gene ATP17. Eur. J. Biochem. 247, 1111-1117.
12. 0 sector of mitochondrial ATP synthase. Trends Genet. 4, 46-52.
13. +-ATPase in mutants of Saccharomyces cerevisiae with defective subunit 8 of the enzyme complex. Biochim. Biophys. Acta 975, 222-230.
14. 0 sector of ATP synthase from Saccharomyces cerevisiae. Stimulation of ATP synthase by subunit-8-reactive antibodies and inhibition by subunit-9-reactive antibodies. Eur. J. Biochem. 223, 521-528.
15. 15. Gearing, D.P. & Nagley, P. (1986) Yeast mitochondrial ATPase subunit 8, normally a mitochondrial gene product, expressed in vitro and imported back into the organelle. EMBO J. 5, 3651-3655.
16. 16. Nagley, P., Farrell, L.B., Gearing, D.P., Nero, D., Meltzer, S. & Devenish, R.J. (1988) Assembly of functional proton-translocating ATPase complex in yeast mitochondria with cytopiasmically synthesized subunit 8, a polypeptide normally encoded within the organelle. Proc. Natl Acad. Sci. USA 85, 2091-2095.
17. 17. Nagley, P. & Devenish, R.J. (1989) Leading organellar proteins along new pathways: the relocation of mitochondrial and chloroplast genes to the nucleus. Trends Biochem. Sci. 14, 31-35.
18. 0 sector. Eur. J. Biochem. 199, 203-209.
19. 19. Papakonstantinou, T., Galanis, M., Nagley, P. & Devenish, R.J. (1993) Each of three positively-charged amino acids in the C-terminal region of yeast mitochondrial ATP synthase subunit 8 is required for assembly. Biochim., Biophys. Acta 1144, 22-32.
20. 20. Devenish, R.J., Papakonstantinou, T., Galanis, M., Law, R.H.P., Linnane, A.W. & Nagley, P. (1992) Structure/function analysis of yeast mitochondrial ATP synthase subunit 8. Ann. NY Acad. Sci. 671, 403-414.
21. 21. Velours, J., Esparza, M., Hoppe, J., Sebald, W. & Guerin, B. (1984) Amino acid sequence of a new mitochondrially synthesized proteolipid of the ATP synthase of Saccharomyces cerevisiae. EMBO J. 3, 207-212.
22. 22. Nagley, P., Devenish, R.J., Law, R.H.P., Maxwell, R.J., Nero, D. & Linnane, A.W. (1990) Subunit 8 of yeast mitochondrial ATP synthase: biochemical genetics and membrane assembly. In Bioenergetics, Molecular Biology, Biochemistry and Pathology (Kim, C.H. & Ozawa, T., eds), pp. 305-325. Plenum Press, New York.
23. 23. Papakonstantinou, T., Law, R.H.P., Manon, S., Devenish, R.J. & Nagley, P. (1995) Relationship of subunit 8 of yeast ATP synthase and the inner mitochondrial membrane. Subunit 8 variants containing multiple lysine residues in the central hydrophobic domain retain function. Eur. J. Biochem. 227, 745-752.
24. 24. Papakonstantinou, T., Law, R.H.P., Nesbitt, W.S., Nagley, P. & Devenish, R.J. (1996) Molecular genetic analysis of the central hydrophobic domain of subunit 8 of yeast mitochondrial ATP synthase. Curr. Genet. 30, 12-18.
25. 25. Galanis, M., Law, R.H.P., O'Keeffe, L.M., Devenish, R.J. & Nagley, P. (1990) Aberrant mitochondrial processing of chimaeric import precursors containing subunits 8 and 9 of yeast mitochondrial ATP synthase. Biochem. Int. 22, 1059-1066.
26. 26. Galanis, M., Devenish, R.J. & Nagley, P. (1991) Duplication of leader sequence for protein targeting to mitochondria leads to increased import efficiency. FEBS Lett. 282, 425-430.
27. 27. Gray, R.E., Law, R.H.P., Devenish, R.J. & Nagley, P. (1996) Allotopic expression of mitochondrial ATP synthase genes in the nucleus of Saccharomyces cerevisiae. Methods Enzymol. 264, 369-389.
28. 28. Proudlock, J.W., Haslam, J. & Linnane, A.W. (1971) Biogenesis of mitochondria. 19. The effects of unsaturated fatty acid depletion on the lipid composition and energy metabolism of a fatty acid desaturase mutant of Saccharomyces cerevisiae. J. Bioenerg. 2, 327-349.
29. 29. Law, R.H.P., Manon, S., Devenish, R.J. & Nagley, P. (1995) ATP synthase from Saccharomyces cerevisiae. Methods Enzymol. 260, 133-163.
30. - Mutations in the oli2 region of mitochondrial DNA affecting the 20 000 dalton subunit of the mitochondrial ATPase in Saccharomyces cerevisiae. FEBS Lett. 108, 501-504.
31. 31. Bateson, M., Devenish, R.J., Nagley, P. & Prescott, M. (1996) Entrapment by immobilised metal ion affinity of assembled yeast mitochondrial ATP synthase containing individual subunits tagged with hexahistidine. Analyt. Biochem. 238, 14-18.
32. 32. Emaus, R.K., Grunwald, R. & Lemasters, J.J. (1986) Rhodamine 123 as a probe of transmembrane potential in isolated rat liver mitochondria: spectral and metabolic properties. Biochim. Biophys. Acta 850, 436-448.
33. 1) from yeast mitochondria. Purification, amino-acid composition and partial N-terminal sequence. Eur. J. Biochem. 164, 579-584.
34. +-ATPase. Biochim. Biophys. Acta 765, 258-267.
35. 0 sector of yeast mitochondrial ATP synthase, in the assembly of the whole complex. Eur. J. Biochem. 185, 163-171.
36. 36. Tzagoloff, A. & Dieckmann, C.L. (1990) PET genes of Saccharomyces cerevisiae. Microbiol. Rev. 54, 211-225.
37. 37. Norais, N., Prome, D. & Velours, J. (1991) ATP synthase of yeast mitochondria: characterization of subunit d and sequence analysis of the structural gene ATP7. J. Biol. Chem. 266, 16541-16549.
38. 38. Tzagoloff, A. (1970) Assembly of the mitochondrial membrane system. 3. Function and synthesis of the oligomycin sensitivity-conferring protein of yeast mitochondria. J. Biol. Chem. 245, 1545-1551.
39. 39. Belogrudov, G.I., Tomich, J.M. & Hatefi, Y. (1995) ATP synthase complex: proximities of subunits in bovine submitochondrial particles. J. Biol. Chem. 270, 2053-2060.
40. 40. Velours, J. & Guerin, B. (1986) Localisation of the hydrophilic C terminal part of the ATP synthase subunit 8 of Saccharomyces cerevisiae. Biochem. Biophys. Res. Commun. 138, 78-86.