COMBINATORIAL SCANNING SITE-DIRECTED MUTAGENESIS

Gene Cloning and Analysis: Current Innovations: Volume 4 in the Current Innovations in Molecular Biology series

Volumn , Issue , 2023, Pages 117-132

  Chatellier, Jean ^a   Vernet, Thierry ^b  

^a CNRS   (France)

^b INSTITUT DE BIOLOGIE STRUCTURALE   (France)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 0004423595     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1201/9781003421474-8     Document Type: Chapter

References (31)

1. Chatellier, J., Mazza, A., Brousseau, R. and Vernet, T. 1995. Codon-based combinatorial alanine scanning mutagenesis: Design, implementation and PCR screening. Anal. Biochem. 229: 282-290.
2. Reidhaar-Olson, J.F. and Sauer, R.T. 1988. Combinatorial cassette mutagenesis as a probe of the informational content of protein sequences. Science. 241: 53-57.
3. Gregoret, L.M. and Sauer, R. 1993. Additivity of mutant effects assessed by binomial mutagenesis. Proc. Natl. Acad. Sci. USA 90: 4246-4250.
4. Sandberg, W.S. and Terwilliger, T.C. 1993. Engineering multiple properties of a protein by combinatorial mutagenesis. Proc. Natl. Acad. Sci. USA. 90: 8367-8371.
5. Smith, M. (1985). In vitro mutagenesis. Annu. Rev. Genet. 19: 423-462.
6. Shortle, D. and Sondek, J. 1995. The emerging role of insertions and deletions in protein engineering. Curr. Opin. Biotechnol. 6: 387-393.
7. Matthews, J.M. and Fersht, A.R. 1995. Exploring the energy surface of protein folding by structure-reactivity relationships and engineered proteins: Observation of Hammond behavior for the gross structure of the transition state and anti-Hammond behavior for the structural elements for unfolding/folding of barnase. Biochemistry. 34: 6805-6814.
8. Lee, G.F., Dutton, D.P. and Hazelbauer, G.L. 1995. Identification of functionnally important helical faces in transmembrane segments by scanning mutagenesis. Proc. Natl. Acad. Sci. USA. 92: 5416-5420.
9. Clarke, J., Hounslow, A.M. and Fersht, A.R. 1995. Disulfide mutants of barnase. II. Changes in structure and local stability identified by hydrogen exchange. J. Mol. Biol. 253: 505-513.
10. Pakula, A.A. and Simon, M.I. 1992. Determination of transmembrane protein structure by disulfide cross-linking: The Escherichia coli Tar receptor. Proc. Natl. Acad. Sci. USA. 89: 4144-4148.
11. Cunningham, B.C. and Wells, J.A. 1989. High resolution epitope mapping of hGH-receptor interactions by alanine-scanning mutagenesis. Science. 244:1081-1085.
12. Wells, J.A. 1990. Additivity of mutational effects in proteins. Biochemistry. 29: 8509-8517.
13. Fersht, A.R., Shi, J.-R, Knill-Jones, J., Lowe, D.M., Wilkinson, A.J., Blow, D.M., Brick, P., Carter, P., Waye, M.M. Y. and Winter, G. 1985. Hydrogen bonding and biological specificity analysed by protein engineering. Nature. 314: 235-238.
14. Horwitz, B.H. and DiMaio, D. 1990. Saturation mutagenesis using mixed oligonucleotides and M13 templates containing uracil. Methods Enzymol. 185: 599-611.
15. Glaser, S.M., Yelton, D.E. and Huse, W.D. 1992. Antibody engineering by codon-based mutagenesis in a filamentous phage vector system. J. Immunol. 149: 3903-3913.
16. Cormack, B.P. and Struhl, K. 1993. Regional codon randomization: Defining a TATA-binding protein surface required for RNA polymerase III transcription. Nature. 262: 244-248.
17. Chatellier, J., Rauffer-Bruyère, N., Van Regenmortel, M.H.V., Altschuh, D. and Weiss, E. 1996. Comparative interaction kinetics of two recombinant Fabs and of the corresponding antibodies directed to the coat protein of tobacco mosaic virus. J. Mol. Rec. 9:39-51.
18. Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989. Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York.
19. Zhang, S., Zubay, G. and Goldman, E. 1991. Low-usage codons in Escherichia coli, yeast, fruit fly and primates. Gene. 105: 61-72.
20. Gait, M.J. (ed.) 1984. Oligonucleotide Synthesis. A Practical Approach. IRL Press, Oxford, England.
21. Sánchez-Pescador, R. and Urdea, M.S. 1984. Use of unpurified synthetic deoxynucleotide primers for rapid dideoxynucleotide chain termination sequencing. DNA. 3: 339-343.
22. Carter, P. 1991. Mutagenesis facilitated by the removal or the introduction of unique restriction sites. In: Directed Mutagenesis. A Practical Approach. McPherson, M.J., ed. IRL Press, Oxford, England, p. 1-25.
23. Yuckenberg, P.D., Witney, F, Geisselsoder, J. and McClary J. 1991. Site-directed in vitro mutagenesis using uracil-containing DNA and phagemid vectors. In: Directed Mutagenesis. A Practical Approach. McPherson, M.J., ed. IRL Press, Oxford, England, p. 27-48.
24. Kunkel, T.A. 1985. Rapid and efficient site-specific mutagenesis without phenotypic selection. Proc. Natl. Acad. Sci. USA. 82: 488-492.
25. Kunkel, T.A., Roberts, J. and Zakour, R. 1987. Rapid and efficient site-specific mutagenesis without phenotypic selection. Methods Enzymol. 154: 367-382.
26. Hanahan, D. 1983. Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166: 557-580.
27. Zoller, M.J. and Smith, M. 1982. Oligonucleotide-directed mutagenesis using M13-derived vectors: An efficient and general procedure for the production of point mutations in any fragment of DNA. Nucl. Acids Res. 10: 6487-6500.
28. Innis, M.A., Gelfand, D.H., Sninsky, JJ. and White, T.J. (eds). 1990. PCR Protocols. A Guide to Methods and Applications. Academic Press, Inc., San Diego, California.
29. Sanger, R, Nicklen, S. and Coulson, A.R. 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. USA. 74: 5463-5467.
30. Sondek, J. and Shortle, D. 1992. A general strategy for random insertion and substitution mutagenesis: Substoichiometric coupling of trinucleotide phosphoramidites. Proc. Natl. Acad. Sci. USA. 89: 3581-3585.
31. Virnekás, B., Ge, L., Plückthun, A., Schneider, K.C., Wellnhofer, G. and Moroney, S.E. 1994. Trinucleotide phosphoramidites: Ideal reagents for the synthesis of mixed oligonucleotides for random mutagenesis. Nucl. Acids Res. 22: 5600-5607.